생명과학회지 (Journal of Life Science)

  • 제19권12호
  • /
  • Pages.1808-1814
  • /
  • 2009
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

생약복합조성물(HemoHIM)의 사람 비만세포주 활성 억제 효과

Inhibitory Effects of a Herbal Composition (HemoHIM) on the Activation of Human Mast Cell Line (HMC-1)

  • 김종진 (순천대학교 생명산업과학대학) ;
  • 조성기 (한국원자력연구원 정읍 방사선과학연구소 방사선생명공학연구부) ;
  • 정우희 (한국원자력연구원 정읍 방사선과학연구소 방사선생명공학연구부) ;
  • 박혜란 (한국원자력연구원 정읍 방사선과학연구소 방사선생명공학연구부) ;
  • 이성태 (순천대학교 생명산업과학대학)
  • Kim, Jong-Jin (Dept. of Biology, College of Life Science and natural Resources, Sunchon National University) ;
  • Jo, Sung-Kee (Radiation Research Division for Bio-Technology, Advanced Radiation Technology Institute, Jeongeup campus of Korea Atomic Energy Research Institute) ;
  • Jung, U-Hee (Radiation Research Division for Bio-Technology, Advanced Radiation Technology Institute, Jeongeup campus of Korea Atomic Energy Research Institute) ;
  • Park, Hae-Ran (Radiation Research Division for Bio-Technology, Advanced Radiation Technology Institute, Jeongeup campus of Korea Atomic Energy Research Institute) ;
  • Yee, Sung-Tae (Dept. of Biology, College of Life Science and natural Resources, Sunchon National University)
  • 발행 : 2009.12.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

방사선에 대한 방호와 면역기능 조절을 목적으로 새로운 생약복합조성물인 HemoHIM을 개발하였다. 식품 원료로 사용 가능한 생약재 3종 당귀, 천궁, 백작약의 에탄올 분획을 열수추출물에 첨가하여 HemoHIM을 제조하였다. HemoHIM의 항알레르기 효과를 검증하기 위하여 사람 비만세포주 HMC-1을 사용해 compound 48/80으로 유도되는 히스타민 분비량과 PMA/A23187로 유도되는 염증성 사이토카인의 분비량을 측정하였다. 히스타민의 양은 형광분석법으로, 염증성 사이토카인 IL-6, IL-8, TNF-$\alpha$, GM-CSF의 양은 효소결합 면역측정법으로 측정하였다. 저농도의 HemoHIM에 의해 히스타민 분비량이 억제되었고, 모든 농도에서 IL-6, TNF-$\alpha$, GM-CSF의 분비량은 억제되었지만 IL-8은 고농도에서만 억제되었다. 사이토카인의 mRNA 발현량은 HemoHIM의 농도 의존적으로 억제되었다. 그리고 c-kit와 Fc$\varepsilon$RI의 mRNA 발현량도 모든 농도에서 억제되었지만, tryptase의 mRNA 발현량은 저 농도에서만 억제되었다. 이상의 결과로 HemoHIM이 비만세포의 활성을 억제하는 효과가 있다는 것을 알 수 있었으며, 상대적으로 독성이 적은 항알레르기 제재로 개발할 가능성을 제시한 것으로 생각된다.

In our previous study, a new herbal preparation (HemoHIM) was developed as a functional food for the radioprotection and immunomodulatory agents. In order elucidate the mechanism involved, we examined the effect of HemoHIM on the compound 48/80-induced histamine release, and on the phorbol 12-myristate 13-acetate (PMA)/calcium ionophore (A23187)-induced inflammatory cytokine secretion in HMC-1. The cell culture supernatants were harvested, and the cytokines (IL-4, IL-6, IL-8, TNF-$\alpha$, GM-CSF) in the supernatants were measured by enzyme-linked immunosorbent assay. The total RNA of the cells was extracted, and the cytokines or c-kit/tryptase/Fc$\varepsilon$RI's messenger RNA expressions were examined using reverse transcriptase polymerase chain reaction. Under low concentrations, HemoHIM inhibited histamine release in HMC-1 stimulated compound 48/80. Furthermore HemoHIM inhibited PMA/A23187-induced inflammatory cytokines' secreation or mRNA expression in a dose-dependent manner. But IL-8 secretion was not inhibited by low concentrayion of HemoHIM, respectively. The mRNA expression of c-kit and Fc$\varepsilon$RI were also inhibited in a dose-dependent manner. Tryptase mRNA expression was only inhibited by low concentration of HemoHIM. These results indicated that HemoHIM might be an useful agent for protection against allergy as well as immune modulation, especially since it is a relatively nontoxic natural product.

키워드

참고문헌

  1. Arend, W. P. and J. M. Dayer. 1995. Inhibition of the production and effects of interleukin-1 and tumor necrosis factor alpha in rheumatoid arthritis. Arthritis Rheum. 38, 151-160 https://doi.org/10.1002/art.1780380202
  2. Barnes, P. J. and I. Adcock. 1993. Anti-inflammatory actions of steroids: molecular mechanisms. Trends Phannacol. Sci. 14, 436-441 https://doi.org/10.1016/0165-6147(93)90184-L
  3. Butler, D. M., R. N. Maini, M. Feldmann, and F. M. Brennan. 1995. Modulation of proinflammatory cytokine release in rheumatoid synovial membrane cell cultures. Comparison of monoclonal anti TNF-alpha antibody with the interleukin-1 receptor antagonist. Eur. Cytokine Netw. 6, 225-230
  4. Caufield, J. P., R. A. Lois, A. Hein, and K. F. Austen. 1980. Secretion in dissociated human plumanary mast cells. Evidence for solubilization of granule contents before discharge. J. Cell BioI. 85, 29-37
  5. Erchler, W. B. and E. T. Keller. 2000. Age-associated increased interleukin-6 gene expression, late-life diseases, and frailty. Annu. Rev. Med. 51, 245-270 https://doi.org/10.1146/annurev.med.51.1.245
  6. Harada, A., N. Mukaida, and K. Matsushima. 1996. Interleukin 8 as a novel target for intervention theraphy in acute inflammatory disease. Mol. Med. Today 2, 482-489 https://doi.org/10.1016/1357-4310(96)10042-3
  7. Jo, S. K., Y. B. Yu, H. Oh, S. R. Kim, and S. H. Kim. 2000. The effects of Shi-Quan-Dai-Bu-Tang and its ingredients on the survival of jejunal crypt cells and hematopoietic cells in irradiated mice. J. Korean Soc. Food Sci. Nutr. 29, 93-98
  8. Jo, S. K., H. R. Park, U. H. Jung, H. Oh, S. H. Kim, and S. E. Lee. 2005. Protective effect of a herbal preparation (HemoHIM) on the self-renewal tissues and immune system against $\gamma$-Irradiation. J. Korean Soc. Food Sci. Nutr. 34, 805-813 https://doi.org/10.3746/jkfn.2005.34.6.805
  9. Kang, K. J., B. D. Jun, O. H. Chai, and M. S. Lee. 1993. Inhibitory effects of Mori cortex on compound 48/80-induced histamine release and calcium uptake of rat peritoneal mast cells. Korean J. Immunol. 15, 91-99
  10. Kang, O. K., H. S. Chae, J. H. Choi, H. J. Choi, P. S. Park, S. H. Cho, G. H. Lee, H. Y. So, Y. K. Choo, O. H. Kweon, and D. Y. Kwon. 2006. Effect of the Schisandra fructus water extract on cytokine release from human mast cell line. J. Med. Food 9, 480-486 https://doi.org/10.1089/jmf.2006.9.480
  11. Kay, A. B. 2006. The role of T lymphocyte in asthma. Chem. Immunol. Allergy 91, 59-75
  12. Kim, S. H., S. E. Lee, H. Oh, S. R. Kim, S. T. Yee, Y. B. Yu, M. W. Byun, and S. K. Jo. 2002. The radioprotective effects of Bu- Zhong-Yi-Qi-Tang: A prescription of traditional Chinese medicine. Am. J. Chin. Med. 30, 127-137 https://doi.org/10.1142/S0192415X02000144
  13. Kim, S. H., S. E. Lee, H. Oh, J. A. Yang, C. Y. Chung, J. S. Jang, Y. B. Yu, and S. K. Jo. 1999. The radioprotective effect of Kuei-Pi- Tang as a prescription of traditional Chinese medicine in mice. J. Korean Soc. Food Sci. Nutr. 28, 698-704
  14. Kim, S. H., M. R. An, S. Y. Nah, J. H. Lee, J. H. Kim, S. K. Jo, S. J. Jang, and D. H. Shin. 2001. The effects of herbs on the radiation-induced apoptosis in intestinal crypt cells. J. Korean Assoc. Radiat. Prot. 26, 27-33
  15. Kim, S. H., H. Oh, S. R. Kim, S. K. Jo, M. W. Byun, K. S. Kim, J. H. Lee, and D. H. Shin. 2001. The radioprotective effects of radices herbs. Korea J. Vet. Res. 41, 105-111
  16. Kim, S. H., H. Oh, E. S. Lee, S. K Jo, and M. W. Byun. 1998. Effect of Si-Wu-Tang and Si-Jun-Zi-Tang on the survival of jejunal crypt cells and hematopoietic cells in irradiated mice. Korean J. Food Sci. Technol. 30, 888-894
  17. Kim, S. H., D. K. Kim, B. S. Chae, and T. Y. Shin. 2003. Inhibitory effect of Isodon japonicus Hara on mast cell-mediated immediate-type allergic reaction. Kor. J. Pharmacogn. 34, 132-137
  18. Lee, S. W., Y. M. Ye, J. H. Choi, S. H. Kim, C. H. Suh, and D. H. Nahm. et al. 2004. Measurement of serum tryptase in the diagnosis of systemetic allergic reaction. Korean J. Med. 67, 185-189
  19. Lee, S. E., H. Oh, J. A. Yang, S. K. Jo, M. W. Byun, S. T. Yee, and S. H. Kim. 1999. Radioprotective effects of two traditional Chinese medicine prescriptions: Si-Wu-Tang and Si-Jun-Zi-Tang. Am. J. Chin. Med. 27, 387-396 https://doi.org/10.1142/S0192415X99000446
  20. Lee, M. S., Y. G. Ryu, O. K. Chai, K. J. Kang and J. Y. Lee. 1999. Inhibitory effect of polysaccharide fraction from Cortex mori on compound 48/80-induced mast cell activation. Korean J. Immunol. 21, 35-45
  21. Li, G. Z., O. H. Chai, and C. H. Song. 2004. Inhibitory effect of Rubus coreanus on compound 48/80- or anti-DNP IgE-induced mast cell activation. Immune. Network 4, 100-107 https://doi.org/10.4110/in.2004.4.2.100
  22. Makowska, J. S., M. Cieslak, and M. L. Kowalski. 2009. Stem cell factor andits soluble receptor (c-kit) in serum of asthmatic patients-correlation with disease severity. BMC. Pulm. Med. 9, 27-33 https://doi.org/10.1186/1471-2466-9-27
  23. Manning, A. M., F. P. Bell, C. L. Rosenbloom, J. G. Chosay, C. A Simmons, J. L. Northrup, R. J. Shebuski, C. J. Dunn, and D. C. Anderson. 1995. NF-kappa B is activated during acute inflammation in vivo in association with elevated endothelial cell adhesion molecule gene expression and leukocyte recruitment. J. Inflamm. 45, 283-296
  24. Oh, H., H. R. Park, I. Y. Jeong, S. H. Kim, and S. K. Jo. 2002. Protective effects of Paeonia japonica against radiation-induced damage. J. Korean Assoc. Radiat. Prot. 27, 181-188
  25. Papageorgiou, P. S. 2002. Clincal aspects of food allergy. Biochem. Soc. Trans. 30, 901-906 https://doi.org/10.1042/BST0300901
  26. Park, H. R., E. J. Ju, S. K. Jo, U. Jung, S. H. Kim, and S. T. Yee. 2009. Enhanced antitumor efficacy of cisplatin in combination with HemoHIM in tumor-bearing mice. BMC Cancer 9, 1-10 https://doi.org/10.1186/1471-2407-9-1
  27. Park, H. R., S. H. Kim, S. T. Yee, M. W. Byun, and S. K. Jo. 2005. Effects of a herb mixture (HIM-I) on the protection of the hematopoietic-immune system and self-renewal tissues against radiation damage. J. Korean Soc. Food Sci. Nutr. 34, 605-612 https://doi.org/10.3746/jkfn.2005.34.5.605
  28. Pastore, S., E. Fanales-Belasio, C. Albanesi, LM. Chinni, A Giannetti, and G. Girolomoni. 1997. Granulocyte macrophage colony-stimulating factor is overproduced by keratinocytes in atopic dermatitis. Implications for sustained dendritic cell activation in the skin. J. Clin. Invest 99, 3009-3017 https://doi.org/10.1172/JCI119496
  29. Payne, V. and P. C. Kam. 2004. Mast cell tryptase. a review of its physiology and clinical significance. Anaesthesia 59, 695-703 https://doi.org/10.1111/j.1365-2044.2004.03757.x
  30. Platts-Mills, T. 2001. Hypersensitivity-Type I, pp. 323-343, In Roitt, I., J. Brostoff, and D. Male (6th eds.), Immunology, Gower medical publishing
  31. Scheller, S., S. Dworniczak, T. Pogorzelska, M. Rajca, and J. Shani. 2000. Effect of quercetin, caffeic acid and caffeic acid phenylethyl ester, solubilized in non-ionic surfactants, on histamine release in vivo and in vitro. Arzneimittelforschung. 50, 72-76
  32. Seo, U. K., J. I. Lee, J. H. Park, and Y. K. Park. 2008. The ethylacetate extract of north kangwhal (Ostericum koreanum) attenuates the inflammatory responses in PM A/ A23187-stimulated mast cells. Kor. J. Herbology 23, 81-89
  33. Shin, T. Y. and D. K. Kim. 2000. Inhibitory effect of mast cell-dependent anaphylaxis by Gleditsia sinesis. Arch. Pharm. Res. 23, 401-406 https://doi.org/10.1007/BF02975455
  34. Tasaka, K., M. Mio, K. Izushi, and I. Aoki. 1991. Role of the cytoskeletons on $Ca^{2+}$ release from the intracellular $Ca^{2+}$ store of rat peritoneal mast cell Skin. Pharmacol. 4, 43-52
  35. Yoshii, N., M. Mio, M. Akagi, and K Tassaka. 1991. Role of endoplasmic reticulum, an intracellular $Ca^{2+}$ store in histamine release from rat peritoneal mast cell. Immunapharmacol. 21, 13-24 https://doi.org/10.1016/0162-3109(91)90003-H

피인용 문헌

  1. Herbal preparation (HemoHIM) enhanced functional maturation of bone marrow-derived dendritic cells mediated toll-like receptor 4 vol.16, pp.1, 2016, https://doi.org/10.1186/s12906-016-1045-9