생명과학회지 (Journal of Life Science)

  • 제19권10호
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  • Pages.1463-1467
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  • 2009
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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노화촉진마우스의 텔로미어 함량 분석

Amount of Telomeric DNA on Lymphocytes in Senescence Mouse by Quantitative Fluorescence in situ Hybridization

  • 이미랑 (부산대학교 동물생명과학과) ;
  • 도경탁 (부산대학교 동물생명과학과) ;
  • 한정주 (부산대학교 동물생명과학과) ;
  • 문소현 (부산대학교 동물생명과학과) ;
  • 강한석 (부산대학교 동물생명과학과) ;
  • 김선구 (부산대학교 동물생명과학과) ;
  • 신택순 (부산대학교 동물생명과학과) ;
  • 이홍구 (부산대학교 동물생명과학과) ;
  • 황대연 (부산대학교 바이오소재) ;
  • 김용균 (부산대학교 생명환경화학과) ;
  • 손시환 (진주산업대학교 동물생명과학과) ;
  • 최나은 (진주산업대학교 동물생명과학과) ;
  • 김병우 (경상대학교 농업생명과학연구원) ;
  • 조병욱 (부산대학교 동물생명과학과)
  • Lee, Mi-Rang (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Do, Kyoung-Tag (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Han, Jyung-Ju (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Moon, So-Hyun (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Kang, Han-Seok (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Kim, Seon-Ku (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Shin, Teak-Soon (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Lee, Hong-Goo (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Hwang, Dae-Yon (Department of biomaterial science, College of Life Science, Pusan National University) ;
  • Kim, Yong-Gyun (Department of Life science & Environmental Biotechnology, College o fLife Science, Pusan National University) ;
  • Sohn, Sea-Hwan (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Choi, Na-Eun (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Kim, Byeong-Woo (Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Cho, Byung-Wook (Department of Animal Science, College of Life Science, Pusan National University)
  • 발행 : 2009.10.30
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초록

Telomeres, comprised of tandem repeats of TTAGGG sequences, are special nucleoprotein structures that protect and stabilize chromosome ends. These structures form the crux of the telomere concept of aging, senescence and genomic instability. The classic terminal restriction fragment (TRF) analysis to quantify the amount of telomeric DNA is disadvantageous in species containing ultra long telomeres like in mice (100Kb). In this study, we used a more sensitive quantitative fluorescence in situ hybridization (Q FISH) technique to quantify telomeric DNA, and used it as a biological aging marker in mice. 12 litters each of Senescence-Resistant (SAMR1) and -Prone (SAMP1) known as senescence accelerated mouse strains were purchased from Central Lab, Animal Inc. We quantified the amount of telomeric DNA using telomere specific DNA probes on the two strains of male mice at 8 weeks, 18 weeks and 26 weeks of age. The amount of telomeric DNA correlated with aging and age associated changes in body and organ weight between SAMR1 and SAMP1 strains of mice. These data suggest the usefulness of the amount of telomeric DNA as a biological aging marker in human aging studies.

키워드

참고문헌

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