Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Amount of Telomeric DNA on Lymphocytes in Senescence Mouse by Quantitative Fluorescence in situ Hybridization

노화촉진마우스의 텔로미어 함량 분석

  • Lee, Mi-Rang (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Do, Kyoung-Tag (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Han, Jyung-Ju (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Moon, So-Hyun (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Kang, Han-Seok (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Kim, Seon-Ku (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Shin, Teak-Soon (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Lee, Hong-Goo (Department of Animal Science, College of Life Science, Pusan National University) ;
  • Hwang, Dae-Yon (Department of biomaterial science, College of Life Science, Pusan National University) ;
  • Kim, Yong-Gyun (Department of Life science & Environmental Biotechnology, College o fLife Science, Pusan National University) ;
  • Sohn, Sea-Hwan (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Choi, Na-Eun (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Kim, Byeong-Woo (Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Cho, Byung-Wook (Department of Animal Science, College of Life Science, Pusan National University)
  • 이미랑 (부산대학교 동물생명과학과) ;
  • 도경탁 (부산대학교 동물생명과학과) ;
  • 한정주 (부산대학교 동물생명과학과) ;
  • 문소현 (부산대학교 동물생명과학과) ;
  • 강한석 (부산대학교 동물생명과학과) ;
  • 김선구 (부산대학교 동물생명과학과) ;
  • 신택순 (부산대학교 동물생명과학과) ;
  • 이홍구 (부산대학교 동물생명과학과) ;
  • 황대연 (부산대학교 바이오소재) ;
  • 김용균 (부산대학교 생명환경화학과) ;
  • 손시환 (진주산업대학교 동물생명과학과) ;
  • 최나은 (진주산업대학교 동물생명과학과) ;
  • 김병우 (경상대학교 농업생명과학연구원) ;
  • 조병욱 (부산대학교 동물생명과학과)
  • Published : 2009.10.30
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Abstract

Telomeres, comprised of tandem repeats of TTAGGG sequences, are special nucleoprotein structures that protect and stabilize chromosome ends. These structures form the crux of the telomere concept of aging, senescence and genomic instability. The classic terminal restriction fragment (TRF) analysis to quantify the amount of telomeric DNA is disadvantageous in species containing ultra long telomeres like in mice (100Kb). In this study, we used a more sensitive quantitative fluorescence in situ hybridization (Q FISH) technique to quantify telomeric DNA, and used it as a biological aging marker in mice. 12 litters each of Senescence-Resistant (SAMR1) and -Prone (SAMP1) known as senescence accelerated mouse strains were purchased from Central Lab, Animal Inc. We quantified the amount of telomeric DNA using telomere specific DNA probes on the two strains of male mice at 8 weeks, 18 weeks and 26 weeks of age. The amount of telomeric DNA correlated with aging and age associated changes in body and organ weight between SAMR1 and SAMP1 strains of mice. These data suggest the usefulness of the amount of telomeric DNA as a biological aging marker in human aging studies.

Keywords

References

  1. Blackburn, E. H. 1991. structure and function of telomeres. Nature 350, 569-573 https://doi.org/10.1038/350569a0
  2. Blackburn, E. H. 2001. Switching and signaling at the telomere. Cell 106, 661-73 https://doi.org/10.1016/S0092-8674(01)00492-5
  3. Gan, Y., Engelke, K. J., C. A. Brown, and J. L. S. Au. 2001. Telomere amount and length assay. Pharmaceutical Res. 18, 1655-1659 https://doi.org/10.1023/A:1013306109801
  4. Greider, C. W. and E. H. Blackburn. 1985. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 43, 405-413 https://doi.org/10.1016/0092-8674(85)90170-9
  5. Greider, C. W. 1991. Telomeres. Curro Opin. Cell BioI. 3, 444-451 https://doi.org/10.1016/0955-0674(91)90072-7
  6. Greider, C. W. 1999. Telomeres do D-loop-T-loop. Cell 97, 419-22 https://doi.org/10.1016/S0092-8674(00)80750-3
  7. Harley, C. B., A. B. Futcher, and C. W. Greiter. 1990. Telomere shorten during ageing of human fibroblasts. Nature 345, 458-460 https://doi.org/10.1038/345458a0
  8. Harley, C. B. 1991. Telomere loss: mitotic clock or genetic time bomb? Rev. Mutat Res 256, 271-282 https://doi.org/10.1016/0921-8734(91)90018-7
  9. Hosokawa, M., Kasai, R, Higuch,i K, Takeshita, S., Shimizu, K, Hamamoto, H, Honma, A, Irino, M., K Toda, and A Matsumura. 1984. Grading score system : a method for evolution of the degree of senescence in senescence accelerate mouse (SAM). Mech Aging Dev. 26
  10. Hultdin, M., E. Gronlund, K. Norrback, and E. ErikssonLindstrom. Just T, Roos G, 1998. Telomere analysis by fluorescence in situ hybridization and flow cytometry. Nucleic Acids Res. 26, 3651-3656 https://doi.org/10.1093/nar/26.16.3651
  11. Kipling, D. and H. J. Cooke. 1990. Hypervariable ultra-long telomeres in mice. Nature 347, 400-402 https://doi.org/10.1038/347400a0
  12. Olovnikow, A. 1973. A theory of marginotomy. J. Theor. BioI. 41, 181-181 https://doi.org/10.1016/0022-5193(73)90198-7
  13. Pathak, S., A. S. Multani, C. L. Furlong, and S. H. Sohn. 2002. Telomere dynamics, aneuploidy, stem cells, and cancer. Int. J. Oncology 20, 637-641
  14. Slijepcevic, P. 2001. Telomere length measurement by Q-FISH. Methods Cell sci. 23, 17-22 https://doi.org/10.1023/A:1013177128297
  15. SohnI S. H., H. J. Jung, and D. S. Choi. 2008. Amount of telomeric DNA on Pig lymphocytes by Quantitative fluorescence in situ hybridization. J. Anim. Sci. & Technol. (Kor.) 50, 465-474
  16. Starling, J. A., J. Maule, N. D. Hastie, and R. C. Allshire. 1990. Extensive telomere repeat arrays in mouse are hypervariable. Nucleic Acids Res. 18, 6881-6888 https://doi.org/10.1093/nar/18.23.6881
  17. Takeda, T., M. Hosokawa, S. Takeshita, M. Irine, K. Higuchi, T. Matsushita, Y. Tomita, K Yasuhira, H. Hamamoto, K. Shimizu, M. Ishii, and T. Yamamuro. 1981. A new murine model of accelerated senescence. Mech Ageing Dev. 17, 183-194 https://doi.org/10.1016/0047-6374(81)90084-1
  18. Weng, N. P. and R. J. Hodes. 2000. The role of telomerase expression and telomere length maintenance in human and mouse. J. Clinial Immunology. 20, 257-267 https://doi.org/10.1023/A:1017223602293
  19. Wilmut, A. E., W. A. E. Schnieke, J. MeWhir, A. J. Kind, and K. H. S. Campbell. 1997. Viable offspring derived from fetal and adult mammalian cells, Nature 385, 810-813 https://doi.org/10.1038/385810a0
  20. Zijlmans, J. M., U. M. Martens, S. S. Poon, A K Roap, H. J. Tanke, R K Ward, and P. M. Lansdrop. 1997. Telomeres in the mouse have large inter-chromosomal variations in the number of T2AG3 repeats. Proc Natl. Acad. Sci. U.S.A. 94, 7423-7428 https://doi.org/10.1073/pnas.94.14.7423