Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidative Effect of Angelica acutiloba Kitagawa Ethanol Extract

일당귀 에탄올 추출물의 항산화 효과

  • Published : 2009.01.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the antioxidant effect of 80% ethanol extracts from Angelica acutiloba Kitagawa (A. acutiloba Kitagawa) in vitro. The extract was further fractionated subsequently by n-hexane, chloroform, ethylacetate, n-butanol and water. Antioxidative activities of different fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical generation, Rancimat test, thiobarbituric acid (TBA) value, nitrite scavenging activity, inhibition of lipid peroxidation and peroxide value (POV) in linoleic acid in comparison with the commercial antioxidant butylated hydroxy toluene (BHT). Antioxidant activities of n-hexane fraction of Angelico acutiloba Kitagawa ethanol extract were the highest among fractions and were a little less than that of BHT. Nitrite scavenging activity showed the most remarkable effect at pH 12. These results suggest that ethanol extracts of A. acutiloba Kitagawa can be used in natural antioxidant source.

본 연구는 일당귀의 생리활성 및 기능성을 검토하기 위하여 in vitro에서 일당귀 에탄올 추출물과 용매별 분획물에 대한 DPPH radical 소거능, 항산화 지수, 아질산염 소거능, linoleic acid에 대한 항산화 효과, 지질과산화 억제 효과를 측정하여 일당귀의 항산화력을 측정하였다. 일당귀 에탄올 추출물을 n-hexane, chloroform, ethylacerate, n-butanol, water로 계통 분획하여 DPPH radical에 대한 자유기 소거능을 측정한 결과 n-hexane 분획이 $39{\mu}g/ml$로 가장 강한 항산화 활성을 나타내었다. 또한 Rancimat로 측정한 항산화 지수도 분획 중 n-hexane 분획이 1.73으로 가장 높게 나타났으며, 아질산염 소거능에서도 모든 pH에서 n-hexane 분획이 가장 높게 나타났으며, pH 변화에 따른 각 분획의 아질산염 소거 작용은 pH 1.2에서 가장 우수하였다. 지질과산화물 생성 억제 효과는 n-hexane 분획이 87.7%로 가장 높았으나, 양성대조군으로 사용한 BHT의 억제율보다는 낮았다. Linoleic acid에 대한 항산화 효과를 과산화물가로 측정한 결과도 n-hexane의 항산화력이 가장 우수하였고 ethylaceta, chloroform, n-butanol, water 순이었다. 이상의 결과 일당귀 추출물은 항산화 효과를 나타내는 물질을 함유하고 있는 것으로 판단되며, 이 원인물질에 대한 연구가 더 진행되어야 할 것이며, 일당귀 추출물의 in vitro 에서 항산화효능의 우수함을 통해 일당귀의 천연항산화제로서 개발 및 활용성을 기대해 본다.

Keywords

References

  1. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1200 https://doi.org/10.1038/1811199a0
  2. Cha, B. C., E. H. Lee and M. A. Noh. 2005. Antioxidant activity of Smilacis chinae radix. Korean J. Pharmacogn. 36, 195-200
  3. Choi, J. H. and S. K. Oh. 1985. Studies on the anti-aging action of Korean Ginseng. Korean J. Food Sci. Technol. 17, 506-515
  4. Comporti, M. 1987. Glutathione depletion agents and lipid peroxidation. Chem. Phys. Lipids 45, 143-169 https://doi.org/10.1016/0009-3084(87)90064-8
  5. Dakaki, K. 1982. Whahanyakmoolhak. pp. 293-297. Namsadang. Tokyo. Japan
  6. Folkow, B. 1982. Physiological aspects of primary hypertension. Physiol. Rev. 62, 347
  7. Han, C. K. 1983. Effects of Angelicae radix on anemic rabbit and content of decursin. Ph.D. Dissertation, Kyunghee University, Seoul
  8. Han, D. S. 1988. Pharmacognosy. pp. 201-203, Myung Publishing Co., Seoul
  9. Ham, M. S., S. S. Kim, J. S. Hong, J. H. Lee, E. K. Chung, Y. S. Park and H. Y. Lee. 1996. Screening and comparison of active substances of Angelica gigas Nakai produced in Kangwon and Angelica acutiloba Kitagawa produced in Japan. Kor. J. Appl. Microbiol. Biotechnol. 24, 624-629
  10. Hertog, M. G., E. J. Feskens, P. C. Hollman, M. B. Katan and D. Kromhout. 1993. Dietary antioxidant flavonoids and risk of coronary heart disease. The Zutphan elderly study. Lancet 342, 1007-1011 https://doi.org/10.1016/0140-6736(93)92876-U
  11. Joo, K. J. and J. J. Kim. 2002. Oxidative stability and flavor compounds of sesame oils blended with vegetable oils. Korean J. Food Sci. Technol. 34, 499-502
  12. Jung, G. T., I. O. Ju, J. S. Choi and J. S. Hong. 2000. The antioxidative, antimicrobial and nitrite scavenging effects of Schizandra chinensis RUPRECHT (Omija) seed. Korean J. Food Sci. Technol. 32, 928-935
  13. Kang, S. A., J. A. Han., K. H. Jang and R. W. Choue. 2004. DPPH radical scavenger activity and antioxidant effects of Cham-dang-gui(angelica gigas). Korean J. Soc Food Sci Nutr. 33, 1112-1118 https://doi.org/10.3746/jkfn.2004.33.7.1112
  14. Kang, Y. H., Y. K. Park, S. R. Oh and K. D. Moon. 1995. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J. Food Sci. Technol. 27, 978-984
  15. Kato, H., I. E. Lee, N. V. Cheyen, S. B. Kim and F. Hayase. 1987. Inhibitory of nitrosamine formation by nondialyzable melanoidins. Agric. Biol. Chem. 5, 1333-1335
  16. Kim, E. Y., I. H. Baik, J. H. Kim, S. R. Kim and M. R. Rhyu. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J. Food Sci. Technol. 36, 333-338
  17. Kim, H. J., J. T. Bae, J. W. Lee, M. H. Hwangbo, H. G. Im and I. S. Lee. 2005. Antioxidant activity and inhibitive effects on human leukemia cells of edible mushrooms extracts. Korean J. Food Preserv. 12, 80-85
  18. Kim, H. K., Y. E. Kim., J. R. Do., Y. C. Lee and B. Y. Lee. 1995. Antioxidative activity and physiological activity of some Korean medicinal plants. Korean J. Food Sci. Technol. 27, 80-85
  19. Kim, H. S. and S. W. Joung. 2006. Effective components and nitrite scavenging ability of root and leaves a Angelica gigas Nakai. Korean J. Food Cookery Sci. 22, 957-965
  20. Kim, J. M., B. I. Seo and B. H. Byun. 2004. Comparative study of efficacy of Angelicae gigantis radix and Angelicae acutilobae radix extract on analgesic effects, anti-inflammatory effects and arthritis. J. Appl. Oriental Med. 4, 1-13 https://doi.org/10.3742/OPEM.2004.4.1.001
  21. Kim, S. Y., J. H. Kim, S. K. Ki, M. J. Oh and M. Y. Jung. 1994. Antioxidant activities of selected oriental herb extracts. J. Am. Oil. Chem. Soc. 71, 633-640 https://doi.org/10.1007/BF02540592
  22. Lee, J. H. and J. S. Choi. 1993. Influence of some flavonoids on n-nitrosproline formation in vitro and in vivo. J. Korean Soc. Food Nutr. 22, 266-272
  23. Lee, S. O., H. J. Lee, M. H. Yu, H. G. Im and I. S. Lee. 2005. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ulung island. Korean J. Food Sci. Technol. 37, 233-243
  24. Lee, S. O., M. J. Kim, D. K. Kim and H. J. Choi. 2005. Antioxidative activities of temperature-stepwise water extracts from Inonotus obliquus. Korean J. Soc. Food Sci. Nutr. 34, 139-147 https://doi.org/10.3746/jkfn.2005.34.2.139
  25. Lim, J. A., Y. S. Na and S. H. Baek. 2004. Antioxidative activity and nitrite scavenging ability of ethanol extract from Phyllostachys bambusoides. Korean J. Food Sci. Technol 36, 306-310
  26. Min, S. H. and B. R. Lee. 2007. Antioxidant activity of medicinal plant extracts cultivated in Jecheon. Korean J. Food Culture 22, 336-341
  27. Namba, T. 1993. Angelicae Radix. pp. 58-61, In the encyclopedia of wakan-yaku (traditional sino-Japanese medicines) with color pictures, Vol. I, Hoikusha
  28. Ottolenghi, A. 1959. Interaction of ascorbic acid and mitochondrial lipids. Arch. Biochem. Biophy. 79, 355-461 https://doi.org/10.1016/0003-9861(59)90414-X
  29. Paker, L. and A. N. Glazer. 1990. Oxygen radicals in biological systems. pp. 186-343, In Methods in Enzymology. Academic Press, London
  30. Park, K. W., S. R. Choi., H. R. Hong., J. Y. Kim., M. Y. Shon and K. I. Seo. 2007. Biological activities of methanol extract of Angelica gigas Nakai. Korean J. Food Preserv. 14, 655-661
  31. Song, J. C., N. K. Park, H. S. Hur, M. H. Bang and N. I. Baek. 2000. Examination and isolation of natural antioxidants from Korean medicinal plants. Korean J. Medicinal Crop. Sci. 8, 94-101
  32. Walker, E. A., B. Pignatelli and M. Friensen. 1982. The role of phenols in catalysis of nitrosamine formation. J. Sci. Food Agric. 33, 81 https://doi.org/10.1002/jsfa.2740330115
  33. Woo, W. S. 1997. Natural products chemistry. pp. 14-15, Seoul National University Press
  34. Yamada, H., K. Komiyama., H. Kiyohara., J. C. Cyong., Y. Hirakawa and Y. Otsuka. 1990. Structural characterization and anti-tumor activity of a peptic polysaccharide from the roots of Angelica acutiloba. Planta Medica. 56, 182-186 https://doi.org/10.1055/s-2006-960921
  35. Yoon, C. H. 1994. A toxonomic study on the genus Angelica L. in Korea and the adjacent regions. Ph.D. Dissertation, Korea University, Seoul
  36. Yun, K. W. and S. K. Choi. 2004. Antimicrobial activity in 2 Angelica species extracts. Korean J. Plant Res. 17, 278-282

피인용 문헌

  1. Anti-Photoaging Effects of Angelica acutiloba Root Ethanol Extract in Human Dermal Fibroblasts vol.33, pp.2, 2017, https://doi.org/10.5487/TR.2017.33.2.125