Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Inhibitory Effects of Fucoidan on Melanin Synthesis and Tyrosinase Activity

Fucoidan의 멜라닌 합성과 tyrosinase 활성도 억제 효과

  • Jung, Sook-Hee (Department of Preventive Medicine and Institute of Natural Products for Health Promotion, Kosin University) ;
  • Ku, Mi-Jung (Department of Preventive Medicine and Institute of Natural Products for Health Promotion, Kosin University) ;
  • Moon, Hee-Jung (Department of Preventive Medicine and Institute of Natural Products for Health Promotion, Kosin University) ;
  • Yu, Byeng-Chul (Department of Preventive Medicine and Institute of Natural Products for Health Promotion, Kosin University) ;
  • Jeon, Man-Joong (Department of Preventive Medicine and Institute of Natural Products for Health Promotion, Kosin University) ;
  • Lee, Yong-Hwan (Department of Preventive Medicine and Institute of Natural Products for Health Promotion, Kosin University)
  • 정숙희 (고신대학교 의과대학 예방의학교실 및 건강증진천연물연구소) ;
  • 구미정 (고신대학교 의과대학 예방의학교실 및 건강증진천연물연구소) ;
  • 문희정 (고신대학교 의과대학 예방의학교실 및 건강증진천연물연구소) ;
  • 유병철 (고신대학교 의과대학 예방의학교실 및 건강증진천연물연구소) ;
  • 전만중 (고신대학교 의과대학 예방의학교실 및 건강증진천연물연구소) ;
  • 이용환 (고신대학교 의과대학 예방의학교실 및 건강증진천연물연구소)
  • Published : 2009.01.30
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Abstract

Melanogenesis is a physiological process that results in the synthesis of melanin pigments. Tyrosinase is a key enzyme for melanin biosynthesis, and hyperpigmentation disorders are associated with abnormal accumulation of melanin pigments, which can be improved by treatment with depigmenting agents. Among the possible melanin-reducing compounds, tyrosinase inhibitors are most promising for preventing and treating pigmentation disorder and are used as skin-whitening agents in the cosmetic industry. In the present study, the effects of fucoidan on melanogenesis and tyrosinase activity of B16F10 melanoma cells were investigated. Melanin synthesis and tyrosinase activity in B16F10 melanoma cells were decreased in a dose-dependent manner by fucoidan. Melanin production and tyrosinase activity in B16F10 melanoma cells stimulated by a-melanocyte stimulating hormone (a-MSH) were inhibited by fucoidan with a dose-dependent manner compared to control. Fucoidan inhibited tyrosinase activity of B16F10 melanoma cells with a dose-dependent manner as assessed by 3,4-dihydroxyphenylalanine (DOPA) staining. In conclusion, these findings indicate that fucoidan, which inhibit melanin synthesis and tyrosinase activity, is an effective skin-whitening agent.

미역, 다시마 등 갈조류의 추출물로서 생리활성성분으로 각광 받고 있는 fucoidan이 멜라닌 합성에 미치는 영양을 알아봄으로써 미백제로서의 개발 가능성 여부를 확인하지 위하여 B16F10 melnoma 세포를 이용하여 멜라닌 합성과 tyrosmase 활성도에 대한 실험의 결과 B16F10 melnoma 세포의 멜라닌 생성과 tyrosinase 활성도는 fucoidan의 농도가 증가함에 따라 농도 의존적으로 억제되었다. B16F10 melnoma 세포에 ${\alpha}$-MSH를 투여 후 멜라닌 생성과 tyrosinase의 활성도 역시 fucoidan의 농도가 증가할수록 멜라닌 생성과 tyrosmase 활성도가 억제되는 경향을 보였다. 또한 DOPA 염색의 결과 fucoidan은 농도 의존적으로 tyrosinase 활성도를 억제하였다. 이상의 결과 해조 추출 다당류인 furosinase은 B16F10 melanoma 세포의 멜라닌 합성과 tyrosinase 활성도 억제 작용을 나타내므로 미백 활성 물질로서의 가능성을 가지고 있는 것으로 생각된다.

Keywords

References

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