Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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A Simple Method for Isolation of Polymannuronate and Polyguluronate from Alginate Hydrolyzed by Organic Acids

유기산에 의해 가수분해된 알긴산에서 폴리만뉴론산과 폴리글루론산을 분리하는 간단한 방법

  • Lee, Dong-Soo (Research Center of Biotechnology & Bioengineering, KBP Co., Ltd.) ;
  • Shin, Myung-Kyo (Division of Environment and Energy, Korean Institute of Industrial Technology) ;
  • Pyeun, Jae-Hyeung (Faculty of Food Science Gild Biotechnology, Pukyong National University) ;
  • Lee, Jin-Woo (Department of Biotechnology, Dong-A University)
  • 이동수 (한국바이오폴리머 생물공학연구소) ;
  • 신명교 (한국생산기술원 환경에너지본부) ;
  • 변재형 (부경대학교 식품생명공학부) ;
  • 이진우 (동아대학교 생명공학과)
  • Published : 2009.01.30
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Abstract

Alginate with a MW of 1,283 kDa was hydrolyzed with 0.4 M organic acids at $100^{\circ}C$ for 3 hr. Molecular weights of alginates hydrolyzed with organic acids ranged from 7.5 to 53.2 kDa. There was no significant difference in the molar ratio of mannuronate to guluronate in alginates hydrolyzed with organic acids. Acetic add was found to be the most effective organic acid for hydrolysis of alginate. The MW of alginate decreased with increasing concentration and reaction time with acetic acid as a hydrolyzing agent. The correlations between the MW of hydrolyzed alginate and concentration of acetic acid as well as reaction time with 0.4 M acetic acid were plotted and the relevant equations obtained in this study. Polymannuronate and polyguluronate were isolated by pH adjustment of alginate hydrolyzed with 0.4 M acetic add. The molar percentages of mannuronate in polymannuronates isolated from alginate hydrolyzed with 0.4 M acetic acid at $100^{\circ}C$ were increasing in proportional to the reaction time such as 75% for 1 hr, 90% for 3 hr, and 98% for 5 hr of reaction time.

분자량이 1,283 kDa인 알긴산을 0.4 M 유기산을 사용하여 $100^{\circ}C$에서 3시간 반응시켜 가수분해하였다. 유기산에 의하여 가수분해된 알긴산의 분자량은 7.5에서 53.2 kDa이었다. 유기산으로 가수분해된 알긴산을 구성하는 만뉴론 산과 글루론산의 비율은 가수분해하지 않은 알긴산과 비교하여 큰 차이가 없었다. 사용한 유기산 중에서 알긴산을 가수분해하는 가장 효과적인 유기산은 초산이었다. 사용한 초산의 농도 및 반응시간이 증가할수록 가수분해된 알긴산의 분자량은 감소하였다. 본 연구를 통하여 가수분해된 알긴산의 분자량과 초산의 농도 및 반응시간과의 상관관계를 나타내는 관계식을 구하였다. 초산으로 가수분해한 알긴산의 pH를 조절하여 폴리만뉴론산과 폴리글루론산을 분리하였다. 초산과 반응하는 시간이 증가할수록 폴리만뉴론산에 존재하는 만뉴론산의 비율은 증가하였다. 폴리만뉴론산에 존재하는 만뉴론산의 비율은 $100^{\circ}C$에서 0.4 M 초산과 1시간, 3시간 및 5시간 반응하였을 경우에 각각 75%, 90% 및 98%이었다.

Keywords

References

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