Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Screening and Purification of an Anti-Prostate Cancer Compound, Deoxypodophyllotoxin, from Anthriscus sylvestris Hoffm

전호(Anthriscus sylvestris Hoffm)로부터 전립선 암세포 저해물질인 deoxypodophyllotoxin 의 탐색 및 분리

  • Cho, Hyo-Jin (Department of Microbiology and Immunology, Pusan National University School of Medicine) ;
  • Yu, Sun-Nyoung (Department of Microbiology and Immunology, Pusan National University School of Medicine) ;
  • Kim, Kwang-Youn (Department of Microbiology and Immunology, Pusan National University School of Medicine) ;
  • Sohn, Jae-Hak (College of Medical and Life Sciences, Silla University) ;
  • Oh, Hyun-Cheol (College of Medical and Life Sciences, Silla University) ;
  • Ahn, Soon-Cheol (Department of Microbiology and Immunology, Pusan National University School of Medicine)
  • 조효진 (부산대학교 의학전문대학원) ;
  • 유선녕 (부산대학교 의학전문대학원) ;
  • 김광연 (부산대학교 의학전문대학원) ;
  • 손재학 (신라대학교 의생명과학대학) ;
  • 오현철 (신라대학교 의생명과학대학) ;
  • 안순철 (부산대학교 의학전문대학원)
  • Published : 2009.01.30
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Abstract

The prostate cancer is the critical health problem, increasing of its related death in worldwide. Unfortunately present surgery and chemotherapeutic choices seem to be impossible in curing or controlling prostate cancer, because metastasis occasionally advances even after these potentially curative therapies. Therefore, there is immediate need to alternative chemoprevention and chemotherapeutic agents. Over one hundred species of dried medicinal herbs were tested for proliferation inhibitory effects on prostate cancer cell line, PC-3. One of them, Anthriscus sylvestris was selected because of potent anti-proliferation effect. The dried root of A. sylvestris was extracted with 100% methanol for 2-3 days and its extract was fractionated by using ethyl acetate. And ethyl acetate layer was subjected to column chromatographies on silica gel, reverse phase-18 (RP-18) and Sephadex LH-20, in turn. Finally, the pure compound was obtained by crystallization in methanol at $4^{\circ}C$ for overnight and identified as deoxypodophyllotoxin by NMR spedorscopic and physico-chemical analyses. In addition, it was confirmed that deoxypodophyllotoxin clearly inhibits the proliferation of PC-3 cells in a dose and time-dependent manner.

전립선암은 현대 남성들에게 걸리기 쉬운 질병으로 나이가 들수록 발병의 위험이 증가하는 질환으로 현재 우리나라에서도 점점 증가하는 추세이다. 전립선암 치료법들은 치료영역이 제한적이고 재발할 가능성이 높아 근본적인 치료법으로는 사용되지는 못하므로 새로운 전립선암 치료 방법이 필요하다. 이에 본 연구에서는 100 여 가지의 한약재 methanol 추출물을 이용하여 MTT 방법으로 전립선암 세포주인 PC-3 세포에 대한 항증식 효과를 탐색하였으며 그 결과, A. sylvestris가 가장 강한 항증식 활성을 보였다. A. sylvestris의 methanol 추출물로부터 저해물질을 분리하기 위하여 100% methanol에서 2-3일 추출하고 난 뒤, ethyl acetate로 추출하고 silica gel, reverse phase-18, Sephadex LH-20 등의 컬럼 크로마토그래피를 이용하여 분리하였다. 최종적으로 활성분획을 HPLC로 분리하고 $4^{\circ}C$에서 methanal 용액에서 입방체 형태의 결정을 얻었으며 NMR 분광법과 이화학적 특성을 분석한 결과, deoxypodophyllotoxin 으로 동정되었다. 순수 분리된 deoxypodophyllotoxin은 전립선암의 세포주 PC-3 세포에서 처리 농도와 처리 시간 의존적인 항증식 효과를 보였다.

Keywords

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