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A Three-Dimensional Particle Focusing Channel Using the Positive Dielectrophoresis (pDEP) Guided by a Dielectric Structure Between Two Planar Electrodes

두 평면 전극 사이의 절연체 구조물에 의해 유도되는 양의 유전영동을 이용한 삼차원 입자 정렬기

  • 추현정 (한국과학기술원 바이오및뇌공학과, 디지털나노구동연구단) ;
  • 도일 (한국과학기술원 바이오및뇌공학과, 디지털나노구동연구단) ;
  • 조영호 (한국과학기술원 바이오및뇌공학과 및 기계공학과, 디지털나노구동연구단)
  • Published : 2009.03.01

Abstract

We present a three-dimensional (3D) particle focusing channel using the positive dielectrophoresis (pDEP) guided by a dielectric structure between two planar electrodes. The dielectric structure between two planar electrodes induces the maximum electric field at the center of the microchannel, and particles are focused to the center of the microchannel by pDEP as they flow from the single sample injection port. Compared to the previous 3D particle focusing methods, the present device achieves the simple and effective particle focusing function without any additional fluidic ports and top electrodes. In the experimental study, approximately 90 % focusing efficiency were achieved within the focusing length of 2mm, on both x-z plane (top-view) and y-z plane (side-view) for $2{\mu}m$-diameter polystyrene (PS) bead at the applied voltage over 15 Vp-p (square wave) and at the flow rate below 0.01 ${\mu}l$/min. The present 3D particle focusing channel results in a simple particle focusing method suitable for use in integrated microbiochemical analysis system.

Keywords

References

  1. Meinhart, C., Wereley, S. and Gray, S., 2000, “Volume Illumination for Two-Dimensional Particle Image Velocimetry,” Meas.Sci.Technol. Vol.11, pp. 809-814 https://doi.org/10.1088/0957-0233/11/6/326
  2. Groisman, A., 2006, “High-Throughput and High-Resolution Flow Cytometry in Molded Microfluidic Devices,” Anal. Chem., Vol.78, pp. 5653-5663 https://doi.org/10.1021/ac060340o
  3. Morgan, H., Holmes, D., and Green, N., 2003, “3D Focusing of Nanoparticles in Microfluidic Channels,” IEE Proc.-Nanobiotech., Vol.150, No.2, pp. 76-81 https://doi.org/10.1049/ip-nbt:20031090
  4. Cheung, K., Gawad, S. and Renaud, P., 2004, “Microfluidic Impedance Spectroscopy Flow Cytometry: Particle Size,” Proc. 17th MEMS 2004, Maastricht, The Netherlands, pp. 343-346 https://doi.org/10.1109/MEMS.2004.1290592
  5. Wang, Z., El-Ali, Z., Engelund, M., Gotsaed, T., Perch-Nielsen, I., Mogensen, K., Snakenborg, D., Kutter, J. and Wolff, A., 2004,“Measurements of Scattered Light on a Microchip Flow Cytometer with Integrated Polymer Based Optical Elements,” Lab chip., Vol.4, pp. 372-377 https://doi.org/10.1039/b400663a
  6. Sundararajan, N., Pio, M., Lee, L., and Berlin, A., 2004, “Three-Dimensional Hydrodynamic Focusing in Polydimethylsiloxane (PDMS) Microchannels,” J. Microelectromech. Syst., Vol.13, No.4, pp. 599-566 https://doi.org/10.1109/JMEMS.2004.832196
  7. Yu, C., Vykoukal, J., Vykoukal, D., Schwartz, J., Shi, L. and Gascoyne, P., 2005, “A Three-Dimensional Dielectrophoretic Particle Focusing Channel for Microcytometry Applications,” J. Microelectromech. Syst., Vol.14, No.3, pp. 480-487 https://doi.org/10.1109/JMEMS.2005.844839
  8. Holmes, D., Morgan, H. and Green, N., 2006, “High Throughput Particle Analysis: Combining Dielectrophoretic Particle Focusing with Confocal Optical Detection,” Biosensor and Bioelectronics, Vol.21, pp. 1621-1630 https://doi.org/10.1016/j.bios.2005.10.017
  9. Hughes, M., 2000, “AC Electrokinetics Applications for Nanotechnology,” Nanotech., Vol.11, pp.124-132 https://doi.org/10.1088/0957-4484/11/2/314

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