DOI QR코드

DOI QR Code

Suppressive Actions of Astragali Radix (AR) Ethanol Extract and Isolated Astragaloside I on HCl/ethanol-Induced Gastric Lesions

  • Published : 2009.01.31

Abstract

Roots of Astragalus membranaceus (A. membranaceus) (Astragali Radix, AR) has been used as a herbal medicine for gastrointestinal disorders in China, Korea, Japan, and other Asia countries. In this study we investigated the effects of the AR 70% ethanol extract and compounds isolated from AR on gastritis in rats and growth of human gastric cancer cells. AR 70% ethanol extract showed the potent acid-neutralizing capacities and partly cytotoxicity of Helicobacter pylori and human AGS gastric cancers cell. Astragaloside I and daucosterol, which were isolated from AR, significantly inhibited HCl/ethanol-induced gastric lesions. In addition, daucosterol increased the mucus content to almost the same as the positive control. The results of this study suggest that astragaloside I and daucosterol may be good candidates for the development of new drugs or neutraceuticals which can be used for the treatment or prevention of gastritis.

Keywords

References

  1. Bae, E. A., Han M. J. and Kim, D. H. (1999). In vitro anti-Helicobacter pylori activity of some flavonoids and their metabolites. Planta. Med. 65, 442-443 https://doi.org/10.1055/s-2006-960805
  2. Cheng, C. Y., Yao, C. H., Liu, B. S., Liu, C. J., Chen, G. W. and Chen, Y. S. (2006). The role of astragaloside in regeneration of the peripheral nerve system. J. Biomed. Mater. Res. 76, 463-469 https://doi.org/10.1002/jbm.a.30249
  3. Cho, W. C. S. and Leung, K. N. (2007a). In vitro and in vivo anti-tumor effects of Astragalus membranaceus. Cancer. Lett. 252, 43-54 https://doi.org/10.1016/j.canlet.2006.12.001
  4. Cho, W. C. S. and Leung, K. N. (2007b). In vitro and in vivo immunomodulating and immunorestorative effects of Astragalus membranaceus. J. Ethnopharmacol. 113, 132-141 https://doi.org/10.1016/j.jep.2007.05.020
  5. Choi, S. I., Heo, T. R., Min, B. H., Cui, J. H., Choi, B. H. and Park, S. R. (2007). Alleviation of osteoarthritis by calycosin-7-O-beta-D-glucopyranoside (CG) isolated from Astragali radix (AR) in rabbit osteoarthritis (OA) model. Osteoarthr. Cartil. 15, 1086-1092 https://doi.org/10.1016/j.joca.2007.02.015
  6. Drake, I. M., Mapstone, N. P., Schorah, C. J., White, K. L., Chalmers, D. M., Dixon, M. F. and Axon, A. T. (1998). Reactive oxygen species activity and lipid peroxidation in Helicobacter pylori associated gastritis: relation to gastric mucosal ascorbic acid concentrations and effect of H. pylori eradication. Gut 42, 768-771 https://doi.org/10.1136/gut.42.6.768
  7. Evans, W. C. (2002).Trease and Evans Pharmacognosy, 15th ed. Elsevier Science Ltd., London
  8. Gerrits, M. M., van Vliet, A. H. M., Kuipers, E. J. and Kusters, J. G. (2006). Helicobacter pylori and antimicrobial resistance: molecular mechanisms and clinical implications. Lancet Infect. Dis. 6, 699-709 https://doi.org/10.1016/S1473-3099(06)70627-2
  9. Gui, S. Y., Wei, W., Wang, H., Wu, L., Sun, W. Y., Chen, W. B. and Wu, C. Y. (2006). Effects and mechanisms of crude astragalosides fraction on liver fibrosis in rats. J. Ethnopharmacol. 103, 154-159 https://doi.org/10.1016/j.jep.2005.07.025
  10. Gyires, K. (2005). Gastric mucosal protection: from prostaglandins to gene-therapy. Curr. Med. Chem. 12, 203-215 https://doi.org/10.2174/0929867053363478
  11. Hirotani, M., Zhou, Y., Rui, H. and Furuya, T. (1994). Astragalosides from hairy root cultures of Astragalus membranaceus. Phytochemistry 36, 665-670 https://doi.org/10.1016/S0031-9422(00)89793-9
  12. Kahraman, A., Erkasap, N., Koken, T., Serteser, M., Aktepe, F. and Erkasap, S. (2003). The antioxidative and antihistaminic properties of quercetin in ethanol-induced gastric lesions. Toxicology 183, 133-142 https://doi.org/10.1016/S0300-483X(02)00514-0
  13. Kim, J. J., Kim, J. G. and Kwon, D. H. (2003). Mixed-infection of antibiotic susceptible and resistant Helicobacter pylori isolates in a single patient and underestimation of antimicrobial susceptibility testing. Helicobacter 8, 202-206 https://doi.org/10.1046/j.1523-5378.2003.00145.x
  14. Kim, J. S., Kim, Y. T. and Kim, C. S. (1996). A study on the constituents from the roots of Astragalus membranaceus. Kor. J. Pharmacogn. 27, 336-341
  15. Kitagawa, H., Takeda, F. and Kohei, H. (1986). A simple method for estimation of gastric mucus and effects of antiulcerogenic agents on the decrease in mucus during water-immersion stress in rats. Arzneimittel Forschung 36, 1240-1244
  16. La Casa, C., Villegas, I., Alarcon de la Lastra, C., Motilva, V. and Martín Calero, M. J. (2000). Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric lesions. J. Ethnopharmacol. 71, 45-53 https://doi.org/10.1016/S0378-8741(99)00174-9
  17. Lee, J. H., Lee, J. Y., Park, J. H., Jung, H. S., Kim, J. S., Kang, S. S., Kim Y. S. and Han Y. M. (2007). Immunoregulatory activity by daucosterol, a ${\beta}$-sitosterol glycoside, induces protective Th1 immune response against disseminated Candidiasis in mice. Vaccine 25, 3834-3840 https://doi.org/10.1016/j.vaccine.2007.01.108
  18. Lee, T. B. (1989). Illustrated Flora of Korea, pp. 491-492. Hyangmunsa, Seoul
  19. Lei, H., Wang, B., Li, W. P., Yang,Y., Zhou, A. W. and Chen, M. Z. (2003). Anti-aging effect of astragalosides and its mechanism of action. Acta Pharmacol. Sin. 24, 230-234
  20. Li, R. J., Qiu, S. D., Chen, H. X., Tian, H. and Wang, H. X. (2007). The Immunotherapeutic effects of Astragalus polysaccharide in type 1 diabetic mice. Biol. Pharm. Bull. 30, 470-476 https://doi.org/10.1248/bpb.30.470
  21. Luo, Y., Qin, Z., Hong, Z., Zhang, X., Ding, D., Fu, J. H., Zhang, W. D. and Chen, J. (2004). Astragaloside IV protects against ischemic brain injury in a murine model of transient focal ischemia. Neurosci. Lett. 363, 218-223 https://doi.org/10.1016/j.neulet.2004.03.036
  22. Matysiak-Budnik, T. and Megraud, F. (2006). Helicobacter pylori infection and gastric cancer. Eur. J. Cancer 42, 708-716 https://doi.org/10.1016/j.ejca.2006.01.020
  23. Mizui, T. and Dodeuchi, M. (1983). Effect of polyamines on acidified ethanol-induced gastric lesion in rats. Jpn. J. Pharmacol. 33, 939-945 https://doi.org/10.1254/jjp.33.939
  24. Navarrete, A., Arrieta, J., Terrones, L., Abou-Gazar, H. and Calis, I. (2005). Gastroprotective effect of Astragaloside IV: role of prostaglandins, sulfhydryls and nitric oxide. J. Pharm. Pharmacol. 57, 1059-1064 https://doi.org/10.1211/0022357056659
  25. Nostro, A., Cellini, L., Di Bartolomeo, S., Di Campli, E., Grande, R., Cannatelli, M. A., Marzio, L. and Alonzo, V. (2005). Antibacterial effect of plant extracts against Helicobacter pylori. Phytother. Res. 19, 198-202 https://doi.org/10.1002/ptr.1640
  26. Rajput, Z. I., Hu, S. H., Xiao, C. W. and Arijo, A. G. (2007). Adjuvant effects of saponins on animal immune responses. J. Zhejiang Univ. Sci. B 8, 153-161 https://doi.org/10.1631/jzus.2007.B0153
  27. Rios, J. L. and Waterman, P. G. (1997). A review of the pharmacology and toxicology of Astragalus. Phytother. Res. 11, 411-418 https://doi.org/10.1002/(SICI)1099-1573(199709)11:6<411::AID-PTR132>3.0.CO;2-6
  28. Seiki, M., Ukei, S., Tanaka, Y. and Soeda, M. (1990). Studies of anti-ulcer effects of a new compound, zinc L-carnosine (Z-103). Nippon Yakurigaku Zasshi 95, 257-269 https://doi.org/10.1254/fpj.95.5_257
  29. Shao, B. M., Xu, W., Dai, H., Tu, P., Li, Z. and Gao, X. M. (2004). A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb. Biochem. Biophys. Res. Commun. 320, 1103-1111 https://doi.org/10.1016/j.bbrc.2004.06.065
  30. Shay, H., Komarov, S. A., Fels, S. S. and Meranze, D. (1945). A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology 4, 43-61
  31. Tepe, B. and Sokmen, A. (2007). Screening of the antioxidative properties and total phenolic contents of three endemic Tanacetum subspecies from Turkish flora. Bioresour. Technol. 98, 3076-3079 https://doi.org/10.1016/j.biortech.2006.10.019
  32. Tin, M. M. Y., Cho, C. H., Chan, K., James, A. E. and Ko, J. K. S. (2007). Astragalus saponins induce growth inhibition and apoptosis in human colon cancer cells and tumor xenograft. Carcinogenesis 28, 1347-1355 https://doi.org/10.1093/carcin/bgl238
  33. Tohda, C., Tamura, T., Matsuyama, S. and Komatsu, K. (2006). Promotion of axonal maturation and prevention of memory loss in mice by extracts of Astragalus mongholicus. Br. J. Pharmacol. 149, 532-541 https://doi.org/10.1038/sj.bjp.0706865
  34. van de Loosdrecht, A. A., Nennie, E., Ossenkoppele, G. J., Beelen, R. H. and Langenhuijsen, M. M. (1991). Cell mediated cytotoxicity against U 937 cells by human monocytes and macrophages in a modified colorimetric MTT assay. A methodological study. J. Immunol. Methods 141, 15-22 https://doi.org/10.1016/0022-1759(91)90205-T
  35. Zhang, W. D., Chen, H., Zhang, C., Liu, R. H., Li, H. L. and Chen, H. Z. (2006a). Astragaloside IV from Astragalus membranaceus shows cardioprotection during myocardial ischemia in vivo and in vitro. Planta Med. 72, 4-8 https://doi.org/10.1055/s-2005-873126
  36. Zhang, W. D., Zhang, C., Liu, R. H., Li, H. L., Zhang, J. T., Mao, C., Moran, S. and Chen, C. L. (2006b). Preclinical pharmacokinetics and tissue distribution of a natural cardioprotective agent astragaloside IV in rats and dogs. Life Sci. 79, 808-815 https://doi.org/10.1016/j.lfs.2006.02.032
  37. Zhang, W. J., Hufnag, P., Binder, B. R. and Wojta, J. (2003). Antiinflammatory activity of astragaloside IV is mediated by inhibition of NF-kappaB activation and adhesion molecule expression. Thromb. Haemost. 90, 904-914

Cited by

  1. Recent progress in phytochemistry, pharmacology and biotechnology of Astragalus saponins vol.13, pp.2, 2014, https://doi.org/10.1007/s11101-014-9347-3
  2. Kazakh Ziziphora Species as Sources of Bioactive Substances vol.21, pp.7, 2016, https://doi.org/10.3390/molecules21070826
  3. Secondary Metabolites of the GenusAstragalus:Structure and Biological-Activity Update vol.10, pp.6, 2013, https://doi.org/10.1002/cbdv.201100444
  4. Inhibitory effect of Sanguisorba officinalis ethanol extract on NO and PGE2 production is mediated by suppression of NF-κB and AP-1 activation signaling cascade vol.134, pp.1, 2011, https://doi.org/10.1016/j.jep.2010.08.060
  5. Anti-gastric Actions of Eugenol and Cinnamic Acid Isolated from Cinnamomi Ramulus vol.131, pp.7, 2011, https://doi.org/10.1248/yakushi.131.1103