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Inheritance and expression of transgene in SOD2-Transgenic petunia descendants and their morphological traits

  • Lee, Su-Young (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Han, Bong-Hee (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Cho, A-Young (National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • Published : 2009.09.30

Abstract

This study was conducted to determine the inheritance and expression of transgene in descendants ($T_1\;to\;T_2$ generation) of SOD2-transgenic petunia by PCR and RT-PCR analysis. The trangene was segregated as Mendelian inheritance pattern (3:1 or 1:0) in most of $T_1\;and\;T_2$ generation lines. Transgenic homozygous lines were obtained in T2 generation. It was identified that the transgene expressed stably in examined all plants of 6 $T_2$ lines. The representative morphological traits (plant height, flower diameter, and flower color) of $T_2$ plants were compared with those of non-transgenic plants.

Keywords

References

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  1. Increase of resistance to oxidative stress induced by methyl viologen in progeny from a cross between two transgenic Petunia lines with NDPK and SOD genes vol.38, pp.3, 2011, https://doi.org/10.5010/JPB.2011.38.3.215
  2. Resistance of SOD2-transgenic petunia line to oxidative stress vol.37, pp.4, 2010, https://doi.org/10.5010/JPB.2010.37.4.562