Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of TIBA, PCIB and phloroglucinol on somatic embryo maturation and germination in Japanese larch (Larix leptolepis)

낙엽송의 체세포배 발생 및 발아에 미치는 TIBA, PCIB 및 phloroglucinol의 효과

  • Kim, Yong-Wook (Division of Biotechnology, Korea Forest Research Institute) ;
  • Moon, Heung-Kyu (Division of Biotechnology, Korea Forest Research Institute)
  • 김용욱 (국립산림과학원 산림생명공학과) ;
  • 문흥규 (국립산림과학원 산림생명공학과)
  • Published : 2009.09.30
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Abstract

The effect of auxin transport inhibitor (TIBA and PCIB) or auxin synergist (phloroglucinol) on somatic embryo maturation and germination in Japanese larch (Larix leptolepis) was examined. The addition of 15.8 mg/L ABA+5.0 mg/L PCIB showed most promoted the maturation of cotyledon -staged somatic embryos (177.7/90 mg ESM). In contrast, with treatment of 5.0 mg/L PCIB or 5.0 mg/L TIBA, no somatic embryos were obtained. Considering from this result, PCIB or TIBA alone could not substitute for exogenously supplied ABA for maturation of somatic embryos. In the test of below concentration of 5.0 mg/L PCIB, the highest results were recorded in 15.8 mg/L ABA+2.0 mg/L PCIB (109.3/90 mg ESM) or 15.8 mg/L ABA+5.0 mg/L PCIB (103.7/90 mg ESM). However, 5.0 mg/L phloroglucinol (0/90 mg ESM) or no ABA addition (3/90 mg ESM) had little influence on somatic embryos maturation. In germination study, the highest frequency of plantlet regeneration obtained from the somatic embryos which had matured on 15.8 mg/L ABA+5.0 mg/L PCIB (67.9%). However, either 5.0 mg/L PCIB nor 5.0 mg/L TIBA resulted in obtained from plantlets.

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References

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