DOI QR코드

DOI QR Code

Red and Blue Photons Can Enhance the Production of Astaxanthin from Haematococcus pluviatis

  • Kim, Z-Hun (Institute of Industrial Biotechnolgy, Department of Biological Engineering, Inha University) ;
  • Lee, Ho-Sang (Institute of Industrial Biotechnolgy,Department of Biological Engineering, Inha University) ;
  • Lee, Choul-Gyun (Institute of Industrial Biotechnolgy,Department of Biological Engineering, Inha University)
  • Published : 2009.06.01

Abstract

The unicellular green alga, Haematococcus pluvialis, accumulates the highest level of astaxanthin among knownastaxanthi.n-producing organisms. Light is the most important factor to induce astaxanthin by H. pluvialis. BIue andred LEDs, whose ${\lambda}_{max}$'s are 470 and 665 nm, respectively, were used for internally illuminated light sources.Fluorescent lamps were also used for both internal and external illumination sources. The astaxanthin levels in thesevarious lighting systems were analyzed and compared each other. The cultures under internally illuminated LEDsaccumulaled 20% more astaxanthin than those under fluorescent lamp. Furthermore, LEDs generated much lessheat than the fluorescent lamps, which gives one more reason for the LEDs being a suitable internal Light source forastaxanthin induction. The results reported here would lead novel designs of photobioreactors with improvementsof illumination methods for high level of astaxanthm production. The maximum astaxanthin concentrations as wellas the astaxanthin yield per supplied photon were increased by at least 20% when blue or red LEDs were supplied.

Keywords

References

  1. Burlew J.S. 1953. Algal Culture from Laboratory to Pilot Plant. Washington, DC, USA, Carnegie Institutiun of Washington Publication. 3-23 pp.
  2. Choi Y.E., Yun Y.S. and Park J.M. 2002. Evaluation of factors promoting astaxanthin production by a unicellular greenalga, Haematococcus pluvialis, with fractional factorial design. Biotechnol. Prog. 18:1170-1175. https://doi.org/10.1021/bp025549b
  3. Geider R.J. and Osborne B.A. 1987. Light absorption by amarine diatom: experimental observation and theoreticalcal culations of the package effect in a small Thalassiosira species. Mar. Biol. 96:299-308. https://doi.org/10.1007/BF00427030
  4. Guerin M., Huntley M.E. and Olaizola M. 2003. Haematococcus astaxanthin: applications for human health and nutrition,Trends Biotechnol. 21: 210-216. https://doi.org/10.1016/S0167-7799(03)00078-7
  5. Kim Z.-H., Kim S.-H., Lee H.-S. and Lee C.-G. 2006. Enhanced production of astaxanthin by flashig light using Haematococcus pluvialis. Enzyme Micob. Technol. 39:414-419. https://doi.org/10.1016/j.enzmictec.2005.11.041
  6. Kobayashi M., Kakizono T., Nishio N. and Nagai S. 1992. Effects of light intensity, light quality, and illuminationcycle on astaxanthin formation in a green alga, Haematococcus pluvialis. J. Ferment. Bioeng. 74:61-63. https://doi.org/10.1016/0922-338X(92)90271-U
  7. Lababpour A., Hada K., Shimahara K., Katsuda T. and Katoh S. 2004. Effects of nutrient supply methods and illuminationwith blue light emitting diodes (LEDs) on astaxanthin production by Haematococcus pluvialis. J, Biosci. Bioeng. 98: 452-456. https://doi.org/10.1016/S1389-1723(05)00311-7
  8. Lee C.-G. 1999. Calculation of light penetration depth in photo-bioreactors. Biotechnol. Bioprocess, Eng. 4:78-81. https://doi.org/10.1007/BF02931920
  9. Lee C.-C. and Palsson B.O. 1994. High-density algal photobiore-actors using light-emitting diodes. Biotechnol. Bioeng. 44:1161-1167. https://doi.org/10.1002/bit.260441002
  10. Lorenz R.T. and Cysewski G.R. 2000. Commercial potential for Haematococcus microalgae as a natural source of astaxan-thin. Trends Biotechnol. 18; 160-167. https://doi.org/10.1016/S0167-7799(00)01433-5
  11. Myers J., Phillips Jr. J.N. and Graham J.-R. 1951. On the massculture of algae. Plant Physiol. 26: 539-548. https://doi.org/10.1104/pp.26.3.539
  12. Orosa M., Franqueira D., Cid A. and Abalde J. 2001. Carotenoid accumulation in Haematococcus pluvialis, in mixotrophic growth. Biotechnol. Lett. 23:373-378. https://doi.org/10.1023/A:1005624005229
  13. Park E.-K. and Lee C.-G. 2001. Astaxanthin production by Haematococcus pluvialis under various light intensities and wavelengths. J. Microbiol. Biotechnol. 11:1024-1030.
  14. Park K.-H. and Lee C.-G. 2000. Optimization of algal photo-bioreactors using flashing lights. Biotechnol. Bioprocess Eng. 5:186-190. https://doi.org/10.1007/BF02936592
  15. Steinbrenner J. and Linden H. 2003. Light inductiun of carotenoid biosynthesis genes in the green alga Haematococcus pluvialis: regulation by photosynthetic redox control. Plant Mol. Biol. 52:343-356. https://doi.org/10.1023/A:1023948929665

Cited by

  1. Specific light uptake rates can enhance astaxanthin productivity in Haematococcus lacustris vol.39, pp.5, 2016, https://doi.org/10.1007/s00449-016-1561-5
  2. Effect of Light Quality on Growth and Fatty Acid Production in Chlorella vugaris Using Light Emitting Diodes vol.8, pp.1, 2016, https://doi.org/10.15433/ksmb.2016.8.1.024
  3. Enhancing biomass and fatty acid productivity of Tetraselmis sp. in bubble column photobioreactors by modifying light quality using light filters vol.22, pp.4, 2017, https://doi.org/10.1007/s12257-017-0200-6
  4. Light wavelength distribution effects on the growth rate of Scenedesmus quadricauda vol.126, 2017, https://doi.org/10.1016/j.bej.2016.09.006