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Characterization of flavonoids specific gene expression in the petals of Dianthus caryophyllus (carnation)

카네이션 (Dianthus caryophillus)의 색소 발현체계 분석

  • Hur, Suel-Hye (Division of Ecological & Environmental System, Kyungpook National University) ;
  • Ahn, Byung-Joon (Division of Biotechnological Resources, Dan Kook University) ;
  • Joung, Hyang-Young (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Hyung, Nam-In (Department of Plant Science and Technology, Sang Myung University) ;
  • Min, Byung-Whan (Division of Ecological & Environmental System, Kyungpook National University)
  • 허설혜 (경북대학교 생태환경대학 생태환경시스템학부) ;
  • 안병준 (단국대학교 생명자원과학대학 환경원예학과) ;
  • 정향영 (국립원예특작과학원 화훼과) ;
  • 형남인 (상명대학교 산업대학 식물산업공학과) ;
  • 민병환 (경북대학교 생태환경대학 생태환경시스템학부)
  • Published : 2009.12.31

Abstract

This study aimed to develop carnation cultivars with new coloring system. We used four genes of Petunia hybrida - chalcone synthase (CHS), flavanone 3-hydroxylase (FHT), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) - as probes, in order to isolate four genes from carnations (Dianthus Caryophyllus). The isolated genes were used as probes in order to select mutants out of collected carnations, using Northern blot analysis. The Northern blot analysis revealed 10 DFR mutants - Gumbyul, Eunbyul, Ballatyne, Crystal, Eugenia, Koreno, Imp. White Sim, West Crystal, White Alpine, and White Charotte. Six among the selected 10 cultivarswere excluded from the target cultivars, because Eugenia, Imp. White Sim, and White Alpine were proved to be double mutants of DFR and ANS, Koreno was considered to be a double mutant of DFR and CHS, and Gumbyul and Ballatyne were proved to be double mutants of DFR and CHI (Chalcone isomerase). Consequently, we selected five DFR mutants, including Virginie, which was already selected as a DFR mutant. Finally, we measured DFR activities in order to confirm the selection, and the results showed that all of the five cultivars - Eunbyul, Crystal, West Crystal, White Charotte, and Virginie - had got no DFR activity.

Keywords

References

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