Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Characterization of flavonoids specific gene expression in the petals of Dianthus caryophyllus (carnation)

카네이션 (Dianthus caryophillus)의 색소 발현체계 분석

  • Hur, Suel-Hye (Division of Ecological & Environmental System, Kyungpook National University) ;
  • Ahn, Byung-Joon (Division of Biotechnological Resources, Dan Kook University) ;
  • Joung, Hyang-Young (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Hyung, Nam-In (Department of Plant Science and Technology, Sang Myung University) ;
  • Min, Byung-Whan (Division of Ecological & Environmental System, Kyungpook National University)
  • 허설혜 (경북대학교 생태환경대학 생태환경시스템학부) ;
  • 안병준 (단국대학교 생명자원과학대학 환경원예학과) ;
  • 정향영 (국립원예특작과학원 화훼과) ;
  • 형남인 (상명대학교 산업대학 식물산업공학과) ;
  • 민병환 (경북대학교 생태환경대학 생태환경시스템학부)
  • Published : 2009.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study aimed to develop carnation cultivars with new coloring system. We used four genes of Petunia hybrida - chalcone synthase (CHS), flavanone 3-hydroxylase (FHT), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) - as probes, in order to isolate four genes from carnations (Dianthus Caryophyllus). The isolated genes were used as probes in order to select mutants out of collected carnations, using Northern blot analysis. The Northern blot analysis revealed 10 DFR mutants - Gumbyul, Eunbyul, Ballatyne, Crystal, Eugenia, Koreno, Imp. White Sim, West Crystal, White Alpine, and White Charotte. Six among the selected 10 cultivarswere excluded from the target cultivars, because Eugenia, Imp. White Sim, and White Alpine were proved to be double mutants of DFR and ANS, Koreno was considered to be a double mutant of DFR and CHS, and Gumbyul and Ballatyne were proved to be double mutants of DFR and CHI (Chalcone isomerase). Consequently, we selected five DFR mutants, including Virginie, which was already selected as a DFR mutant. Finally, we measured DFR activities in order to confirm the selection, and the results showed that all of the five cultivars - Eunbyul, Crystal, West Crystal, White Charotte, and Virginie - had got no DFR activity.

Keywords

References

  1. Brugliera F, Tull D, Holton TA, Karan M, Treloar N, Simpson K, Skurozynska J, and Nason JG (2000) Introduction of a cytochrome b5 enhances the activity of flavonoid 3'5'in transgenic carnation. Sixth International Congress of Plant Molecular Biology. University of Laval, Quebec, 6-8
  2. Davies K, Bloor S and Spiller G (1998) Production of yellow colour in flower: redirection of flavonoid biosynthesis in petunia. Plant J 13:259-266 https://doi.org/10.1046/j.1365-313X.1998.00029.x
  3. Geneve RL, Preece JE and Merkle SA (1997) Flower colour, In: Biotechnology of ornamental plants. GAB International 259-294
  4. Goto T and Kondo T (1991) Structure and molecular stacking of anthocyanins flower colour variation. International Edition in English 30:17-33 https://doi.org/10.1002/anie.199100171
  5. Harborne JB (1988) Flavonoid checklists. In: The Flavonoids. Ed. Harborne, J.B. Chapman and Hall, London, 539
  6. Heller W, Forkmann G, Britsch L and Grisebach H (1985) Enzymatic reduction of dihydroflavonols to flavan-3,4-cis-diols with flower extracts from matthiola incana and its role in anthocyanin biosynthesis. Planta 165:284-287 https://doi.org/10.1007/BF00395052
  7. Heller W and Forkmann G (1994) In: The Flavonoids. 499-536, Chapman and Hall, London
  8. Katsumoto Y, Fukuchi-Mizutani M, Fukui Y, Brugliera F, Holton TA, Karan M, Nakamura N, Yonekura-Sakakibara K, Togami J, Pigeaire A, Ashikari T, Kusumi T, Mason JG and Tanaka Y (2007) Engineering of the rose flavonoid biosynthesis pathway successfully generated blue-hued flowers accumulating delphinidin. Plant Cell Phisiol 48(11):1589-1600 https://doi.org/10.1093/pcp/pcm131
  9. Koes R, De Vetten N and Mol J. (2000) Cytochrome b5 from petunia. PCT-international Patent Application No. WO 00/09720
  10. Krol AR, van der Lenting PE, Veenstra J, van der Meer IM, Koes RE, Gerats AGM, . Mol JNM and Stuitje AR (1988)An antisense chalcone synthase gene in transgenic plants inhibits flower pigmentation. Nature 333:866-869 https://doi.org/10.1038/333866a0
  11. Lee HJ, Nahm SH, Ahn BJ, Joung HY and Min BW (2006) Cloning of cDNA Coding for Dihydroflavonol-4-reductase (DFR) and Characterization of dfr Expression in the Petals of Dianthus caryophyllus (carnation). J. Kor. Hort. Sci. 45:49-54
  12. Logemann J, Schell J and Willmitzer L (1987) Improved method for the isolation of RNA from plant tissue. Anal. Biochem. 163:16-20 https://doi.org/10.1016/0003-2697(87)90086-8
  13. Meyer P, Heidmann I, Forkmann G and Saedler H. (1987)A new petunia flower colour generated by transformation of a mutant with a maize gene. Nature 330:677-678 https://doi.org/10.1038/330677a0
  14. Niesbach-Kloesgen U, Barzen E, Bernhardt J, Rhode W, Schwarz-Sommer Zs, Reif HJ, Wienand U and Saedler H (1987) Chalcone synthase genes in plants: a tool to study evolutionary relationships. J. Mol. Evol. 26:212-225 https://doi.org/10.1007/BF02099854
  15. Pelt JL, Downes WA, Schoborg RV, Mclntosh CA (2003) Flavanone 3-hydroxylase expression in Citrus paradisi and Petunia hybrida seedlings. Phytochem. 64:435-444 https://doi.org/10.1016/S0031-9422(03)00341-8
  16. Sambrook J, Fritsch EF and Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY