The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Identification and Characterization of Paenibacillus polymyxa DY5 with Antifungal Activity against Crop Pathogenic Fungi

작물병원 진균에 대하여 항균 활성을 보이는 Paenibacillus polymyxa DY5의 동정 및 특성

  • Kim, Hyo-Yoon (Department of Biology, Andong University) ;
  • Weon, Hang-Yeon (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Kim, Wan-Gyu (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Yoo, Kwan-Hee (Department of Life Science, Sangji University)
  • 김효윤 (안동대학교 자연과학대학 생명과학과) ;
  • 원항연 (농촌진흥청 국립농업과학원 유전자원센타) ;
  • 김완규 (농촌진흥청 농업과학원 농업미생물과) ;
  • 유관희 (상지대학교 이공과대학 생명과학과)
  • Published : 2009.12.31
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Abstract

A Gram-positive, rod-shaped bacteria named DY5 was isolated from a peat sample collected from Daeam mountain in Korea. The culture filtrate of the bacterial isolate DY5 showed a broad spectrum of antifungal activity on various crop pathogenic fungi such as Trichoderma koningii, Fusarium oxysporum, Colletotrichum gloeosporioides, Sclerotinia sclerotiorum, Rhizoctonia solani AG-1(IA) For the identification of the DY5, morphological, biochemical, API 50 CHB test, analysis of fatty acid and molecular phylogenetic approaches were performed. The DY5 was found to be a member of the genus Paenibacillus on the basis of morphological and biochemical analysis. The 16S rRNA of DY5 showed high similarity(98%) with Paenibacillus polymyxa. On the basis of these results, the DY5 was identified as Paenibacillus polymyxa. Antifungal substance of the DY5 would be mild alkaline proteine molecule. The DY5 seems to have a great potential to be a biocontrol agent against various crop pathogens.

Keywords

References

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