황금(Scutellaria baicalensis)의 에틸 아세테이트 추출물의 항산화 및 항균 활성

Antioxidant and Antibacterial Activities of Ethyl Acetate Extract from Scutellaria baicalensis

  • Kim, Yong-Hyun (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Paek, Jong-Yoon (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Kwon, Hyun-Jung (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Lee, Jae-Woo (Dept. of Food Nutrition, Gimcheon College) ;
  • Yoon, Ok-Hyun (Dept. of Food Nutrition, Gimcheon College) ;
  • Han, Man-Deuk (Dept. of Biology, College of Natural Science, Soonchunhyang University)
  • 발행 : 2009.09.30

초록

This study aimed to determine the antioxidant and antibacterial effects of solvent extracts from Scutellaria baicalensis. The S. baicalensis was successively extracted with 5 kinds of solvents. The obtained samples were as follows; hexan fraction (IPK-1), chloroform fraction(IPK-2), ethyl acetate fraction(IPK-3), butanol fraction(IPK-4), and water fraction(IPK-5). These extracts were determined to process antibacterial effects using a paper disc method against 18 species of human pathogenic bacteria, antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl(DPPH) method and inhibiting ability of linoleic acid autoxidation. Extracts of S. baicalensis has shown selective antibacterial effects on various strains of bacteria. Ethyl acetate fraction(IPK-3) showed the highest antimicrobial activity against selective bacteria, such as Staphylococcus aureus 285, S. aureus 503. In antioxidant activity test, free radical scavenger activity of IPK-3 fraction was 92% compared with BHA at the same concentration, while lipid peroxidation inhibition was 48%. These results suggest that IPK-3 fraction of S. baicalensis could be suitable in development of antimicrobial and antioxidant agents.

키워드

참고문헌

  1. Bae JH, Lee MJ, Lee SM. 2005. Antimicrobial effect of Scutellaria baicalensis Georgi extracts on food-borne pathogens. J Kor Microbiol Biotechnol 33:35-40
  2. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 26:1199-1200 https://doi.org/10.1038/1811199a0
  3. Chi HY, Kim KH, Kong WS, Kim SL, Kim JA, Chung IM, Kim JT. 2005. Antioxidant activity and total phenolic compounds of P. eryngii spp. extracts. Kor J Crop Sci 50:216-219
  4. Cho SH, Kim YR. 2001. Antimicrobial characteristics of Scutellariae Radix extract. J Kor Soc Food Sci Nutr 30:964-968
  5. Choi EM, Lee BK, Koo SJ. 2001. Inhibitory effect on delayedtype hypersensitive by the hot water extracts from medicinal herbs. Kor J Food Sci Technol 33:146-148
  6. Choi MR, Lee JS, Lim HS. 2007. Changes in physiological activities of Scutellariae baicalensis by heating. J Life Sci 17:1381-1386 https://doi.org/10.5352/JLS.2007.17.10.1381
  7. Choi OK, Kim YS, Cho GS, Sung CK. 2002. Screening for antimicrobial activity from Korean plants. Kor J Food Nutr 25:300-306
  8. Halliwell B, Gutteridge JMC. 1984. Oxygen toxicity, oxygen radicals, transition metals and disease. Eur J Biochem 219:1-14 https://doi.org/10.1111/j.1432-1033.1994.tb19909.x
  9. Jeon YH, Kim MH, Kim MR. 2008. Antioxidative, antimutagenic, and cytotoxic activities of ethanol extracts from Cornus officianalis. J Kor Soc Food Sci Nutr 37:1-7 https://doi.org/10.3746/jkfn.2008.37.1.1
  10. Jung EA, Kim NJ, Kim YG, Kim DH, Lee SI. 2001. Studies on the development of antihyperlipidemic drugs from oriental herbal medicines(III)-Antihyperlipidemic effects of Gamigwaruhaebaekwhanggum-Tang and its constituent herbal medicines in vitro. Kor J Pharmacogn 32:22-30
  11. Jung JW, Ahn NY, Park SH, Oh JG, Oh HR, Lee BG, Om AS, Kim BS, Kim DH, Ryu JH. 2004. The anxiolytic-like effects of Scutellaria baicalensis using elevated plus-maze in rats. Kor J Pharm 35:22-27
  12. Kim CH, Jia H, Kim SH, Moon HJ, Lee JS. 2004. The protective effect of Scutellaria baicalensis Georgi on ischemiareperfusion injuries of rat hearts. Kor J Lab Ani Sci 20:357-362
  13. Kim SC, Ahn KS, Park CK, Jeon BS, Lee JT, Park WJ. 2006. Isolation of antioxidative compound from Scutellaria baicalensis G. J Kor Medicinal Crop Sci 14:212-216
  14. Kim YE, Lee YC, Kim HG, Kim CJ. 1997. Antioxidative effect of ethanol fraction for several Korean medicinal plant hot water extracts. Kor J Food & Nutr 10:141-144
  15. Kim YH, Park YS. 2006. Effect of Scutellaria baicalensis water extract on antioxidative activity and epidermal thickness in DNCB-induced allergic contact dermatitis animal model. J Kor Soc Food Sci Nutr 35:543-548 https://doi.org/10.3746/jkfn.2006.35.5.543
  16. Kumaran A, Karunakaran RJ. 2006. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chemistry 97:109-114 https://doi.org/10.1016/j.foodchem.2005.03.032
  17. Lee CK, Seo JJ. 2005. Antimicrobial activity of Whangkumtang extract and Scutellariae Radix extract on the food-borne pathogens. J Kor Soc Food Sci Nutr 34:1606-1610 https://doi.org/10.3746/jkfn.2005.34.10.1606
  18. Lee OH, Kim KY, Jang MK, Yu KH, Yoo BH, Kim SG, Kim MH, Lee SH. 2008. Establishment of the extraction process by evaluation of proanthocyanidin contents and antioxidative activities of pine(Pinus densiflora) needle extracts. J Life Sci 18:992-998 https://doi.org/10.5352/JLS.2008.18.7.992
  19. Maeura Y, Weisburger JH, Williams G. 1984. Dose-dependent reduction of N-2-fluorenylacetamide-induced liver cancer and enhancement of bladder cancer in rats by butylated hydroxytoluene. Cancer Res 44:1604-1610
  20. Moon YH, Lee YH, Min BS, Bae KH. 1997. Antibacterial constituents from Scutellariae Radix against Streptococcous mutans OMZ176. J Kor Pharmacogn 28:99-103
  21. Osawa T. A novel type of antioxidant isolated from leaf wax of Eucalypyus leaves. Agric Biol Chem 45:735-739 https://doi.org/10.1271/bbb1961.45.735
  22. Park SN, Hong JH, Park MG. 1997. Biological protective effect of component from Scutellaria baicalensis Georgi against active oxygen induced-tissue damage(V). Research Bulletin of Seoul-Agriculture University 45:189-199
  23. Rhee JS, Woo ER, Kim NH, Lee EJ, An DK, Lee JH, Park SK, Park HK. 1997. A study on qualitative and quantitative analysis of major ingredients in Scutellariae radix. Anal Sci Technol 10:91-104
  24. Ro HS, Ko WG, Kim OJ, Park KK, Cho YW, Park HS. 1996. Antihyperlipidemic activity of Scutellaric baicalensis Georgi, Coptidis japonica Makino and Rhei koreanum Nakai on exterimental hyperlipidemic in rats. J Kor Pharm 26:215-219
  25. Woo IT, Park KN, Lee SH. 2007. Antimicrobial activities of Scutellaria baicalensis Georgi against various pathogens and spoilage bacterial isolated from Tofu. J Kor Soc Food Sci Nutr 36:470-475 https://doi.org/10.3746/jkfn.2007.36.4.470
  26. Yang JH, Kim YI. 1998. Preparation and antibacterial effects of Scutellariae Radix extract emulsion containing baicalin. J Kor Pharm Sci 28:159-164
  27. Yoon SH, Oh KH, Ha H. 1997. Protective effect of Bupleuri Radix and Sutellariae Radix in chemically induced liver injury. Research Bulletin of Catholic University of Daegu 56:255-262