일품벼/모로베레칸 이입계통을 이용한 농업형질 관련 QTL 분석

Mapping QTLs for Agronomic Traits Using an Introgressin Line Population from a Cross between Ilpumbyeo and Moroberekan in Rice

  • 구홍광 (충남대학교 농업생명과학대학) ;
  • 김동민 (충남대학교 농업생명과학대학) ;
  • 강주원 (충남대학교 농업생명과학대학) ;
  • 김명기 (국립식량과학원 답작과) ;
  • 김연규 (국립식량과학원 답작과) ;
  • 안상낙 (충남대학교 농업생명과학대학)
  • Ju, Hong-Guang (College of Agriculture & Life Sciences, Chumgnam National University) ;
  • Kim, Dong-Min (College of Agriculture & Life Sciences, Chumgnam National University) ;
  • Kang, Ju-Won (College of Agriculture & Life Sciences, Chumgnam National University) ;
  • Kim, Myung-Ki (Rice Research Division, National Institute of Crop Science) ;
  • Kim, Yeon-Gyu (Rice Research Division, National Institute of Crop Science) ;
  • Ahn, Sang-Nag (College of Agriculture & Life Sciences, Chumgnam National University)
  • 투고 : 2008.12.24
  • 발행 : 20081200

초록

We conducted a QTL analysis of agronomic traits using 117 $BC_3F_5$ and $BC_3F_6$ lines developed from a cross between Ilpumbyeo and Moroberekan. Genotypes of 117 $BC_3F_5$ lines were determined using 134 simple sequence repeat (SSR) markers. A total of 832 Moroberekan chromosome segments with 410 homozygous and 422 heterozygous, respectively, were detected, and the genetic distance of introgression segments ranged from 0.5 cm to 112.1 cm. A linkage map constructed using 134 SSR markers was employed to characterize quantitative trait loci (QTL). The 117 $BC_3F_5$ and $BC_3F_6$ lines were evaluated for seven agronomic traits at two locations in 2006 and 2007 and at one location in 2007. A total of 26 QTLs were identified for seven traits including days to heading, and the phenotypic variance explained by each QTL ranged from 9.2% to 24.2%. Moroberekan alleles contributed positive effects in the Ilpumbyeo background at eleven QTL loci including panicle length and spikelets per panicle. Five QTLs, two for days to heading and one each for culm length, panicle length and spikelets per panicle were consistently detected in every occasions indicating that these QTLs are stable. Among them, two QTLs, spp6 for spikelets per panicle and pl6 for paniclel length were localized in the similar region. Increase in spikelets per panicle at this locus might be due to the increase in panicle length, because both traits were associated with increase in spikelets per panicle and panicle length due to the presence of the Moroberekan allele. These Moroberekan QTLs might be useful in breeding programs to develop high-yielding cultivars.

키워드

과제정보

연구 과제 주관 기관 : 작물기능유전체사업단

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