Korean Journal of Metals and Materials (대한금속재료학회지)

The Korean Institute of Metals and Materials (대한금속재료학회)
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Application of Nanoparticles for Materials Recognition Using Peptide Phage Display Technique - Part II: Magnetic Bio-panning Using Fe3O4 Nanoparticles

Peptide phage display 기술을 이용한 나노입자의 materials recognition 응용 - Part II: Fe3O4 나노입자를 이용한 magnetic bio-panning

  • Lee, Chang-Woo (Division of Materials and Chemical Engineering, Hanyang University) ;
  • Kim, Min-Jung (Division of Materials and Chemical Engineering, Hanyang University) ;
  • Standaert, R. (Biological and Nanoscale Systems Group, Oak Ridge National Laboratory) ;
  • Kim, Seyeon (Biological and Nanoscale Systems Group, Oak Ridge National Laboratory) ;
  • Owens, E. (Biological and Nanoscale Systems Group, Oak Ridge National Laboratory) ;
  • Yan, Jun (Biological and Nanoscale Systems Group, Oak Ridge National Laboratory) ;
  • Choa, Yong-Ho (Division of Materials and Chemical Engineering, Hanyang University) ;
  • Doktycz, M. (Biological and Nanoscale Systems Group, Oak Ridge National Laboratory) ;
  • Lee, Jai-Sung (Division of Materials and Chemical Engineering, Hanyang University)
  • Received : 2007.12.20
  • Published : 2008.03.22
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Abstract

The magnetism of$Fe_3O_4$ nanoparticles was applied to magnetic bio-panning process for finding specific sequences against $Fe_3O_4$ crystal phase. Vibrating sample magnetometer (VSM) measurement showed that the coercivity of 30 Oe and the saturation magnetization of 55 emu/g were sufficient in controlling particle movement and magnetizing particles in the media, respectively. This ferrimagnetism of nanoparticles practically enhanced panning efficiency by exaggerating centrifuge step and preventing particle loss. Sequencing results showed that histidine which was commonly found in peptide sequences played an important role in the binding onto $Fe_3O_4$ nanoparticle surface. However, various possible motifs were also observed from several neighboring amino acids of histidine.

Keywords

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