FLOWER RESEARCH JOURNAL (화훼연구)

The Korean Society for Floricultural Science (한국화훼학회)
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Effect of Developmental Stage of Pollen Grain and Temperature Pretreatment on Anther Culturein Lilium Asiatic Hybrid 'Dreamland'

Lilium Asiatic hybrid 'Dreamland'의 약배양에 미치는 화분 발육단계와 온도 전처리의 영향

  • Park, Young Ae (School of Applied Bioscience, Kyungpook National University) ;
  • Hwang, Yoon Jung (School of Applied Bioscience, Kyungpook National University) ;
  • Park, In Sook (School of Applied Bioscience, Kyungpook National University) ;
  • Suh, Dong Hee (School of Applied Bioscience, Kyungpook National University) ;
  • Jeon, Su Min (School of Applied Bioscience, Kyungpook National University) ;
  • Yeo, Kum-Bok (Agro-Biotechnology Education Center, Kyungpook National University) ;
  • Lee, Ga Young (Agro-Biotechnology Education Center, Kyungpook National University) ;
  • Chung, Jae-Dong (School of Applied Bioscience, Kyungpook National University) ;
  • Lim, Ki Byung (School of Applied Bioscience, Kyungpook National University)
  • 박영애 (경북대학교 응용생명과학부) ;
  • 황윤정 (경북대학교 응용생명과학부) ;
  • 박인숙 (경북대학교 응용생명과학부) ;
  • 서동희 (경북대학교 응용생명과학부) ;
  • 전수민 (경북대학교 응용생명과학부) ;
  • 여금복 (경북대학교 생물건강농업생명 융합형 인재양성 사업단) ;
  • 이가영 (경북대학교 생물건강농업생명 융합형 인재양성 사업단) ;
  • 정재동 (경북대학교 응용생명과학부) ;
  • 임기병 (경북대학교 응용생명과학부)
  • Received : 2007.11.28
  • Accepted : 2007.12.18
  • Published : 2008.03.31

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Abstract

This study was carried out to produce haploid plants to verify a systematic breeding program and genetic analysis. Effect of developmental stage of pollen grains and pre-treatment temperature on production of haploid plants was investigated. Microscopic investigation of the explants (Lilium Asiatic hybrid 'reamland' revealed that the length of flower bud at 23.0-24.9, 25.0-26.9, and 27.0-28.9 mm long coincided with tetrad, uninucleate, and binucleate, respectively. When the efficiency of the anther culture from microgametogenetic stages was tested, late uninucleate to early binucleate stage, having the length of 23.0 to 28.0 mm long flower bud, was the best. The frequencies of the callus induction and plant regeneration from the stage mentioned above were 17.8 and 6.7%, respectively. When calli were cultured on the MS medium containing picloram and zeatin at $25^{\circ}C$, shoots were obtained. Roots of regenerated plantlets were confirmed as haploid through an microscopic observation.

나리(Lilium Asiatic hybrid 'Dreamland'의 약배양을 통하여 반수체를 얻어 체계적인 육종방법 및 유전양식을 규명할 목적으로 배양효율에 미치는 화분발육단계와 온도전처리가 미치는 영향에 대한 실험결과는 다음과 같다. 'Dreamland'의 감수분열중인 약을 현미경적방법으로 검경하여 본 결과 화뢰의 길이가 23.0-24.9mm는 tetrad 단계이었고 25.0-26.9mm는 uninucleate 단계, 27.0-28.9mm에서는 binucleate 단계임을 확인할 수 있었으며, 저온 및 고온 온도처리 결과는 전체적으로 캘러스 및 배 발생율은 저조하였고 특이한 점은 발견할 수 없었다. Microgametogenesis 단계별로 약배양 효율을 조사한 결과, late uninucleate-early binucleate 단계인 23.0-28.0mm에서 17.8% callus가 유도되었고, 식물체 재분화율도 6.7%로 나타나 적정 배양 stage임을 확인할 수 있었다. 유기된 캘루스를 picloram과 zeatin을 혼용한 MS 배지에 이식하여 $25^{\circ}C$에서 배양하여 식물체를 얻었다.

Keywords

Acknowledgement

Supported by : 농촌진흥청