Applied Microscopy

  • 제38권3호
  • /
  • Pages.205-212
  • /
  • 2008
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
한국현미경학회 (Korean Society of Microscopy)
권호 표지

6-Aminonicotinamide가 햄스터의 정소에 미치는 영향

Effects of 6-aminonicotinamide on the Testes of Golden Hamster

  • 이진숙 (연세대학교 원주의과대학 해부학교실) ;
  • 최병영 (연세대학교 원주의과대학 해부학교실) ;
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 정원석 (연세대학교 원주의과대학 해부학교실) ;
  • 조병필 (연세대학교 원주의과대학 해부학교실) ;
  • 양영철 (연세대학교 원주의과대학 해부학교실)
  • Lee, Jin-Suk (Department of Anatomy, Yonsei University, Wonju College of Medicine) ;
  • Choi, Byoung-Young (Department of Anatomy, Yonsei University, Wonju College of Medicine) ;
  • Kim, Dong-Heui (Department of Environmental Medical Biology, Yonsei University, Wonju College of Medicine) ;
  • Jung, Won-Sug (Department of Anatomy, Yonsei University, Wonju College of Medicine) ;
  • Cho, Byung-Pil (Department of Anatomy, Yonsei University, Wonju College of Medicine) ;
  • Yang, Young-Chul (Department of Anatomy, Yonsei University, Wonju College of Medicine)
  • 발행 : 2008.09.30
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초록

6-aminonicotinamide (6-AN)의 햄스터 정소에 미치는 영향을 확인하기 위해 실험군에는 체중 kg 당 10 mg의 6-AN을, 대조군에는 동량의 생리식염수를 격일로 복강 투여한 후 정소의 변화를 광학 및 투과전자현미경을 이용하여 관찰하였다. 최초 6-AN 투여 당시의 체중에 비해 7회 투여군부터 유의하게 체중이 감소하였으며, 정소 중량의 감소는 5회 투여시 크게 감소하여 이후 비슷한 양상을 보였다. 정세관상피의 퇴행변화는 5회 투여군부터 나타나기 시작하여 9회 투여군부터는 대부분의 정세관이 심하게 손상을 받은 것으로 나타났다. 손상을 받은 정세관에서 정세관상피를 이루는 정자발생세포 및 지지세포 모두 심한 공포화에 따른 세포의 파괴를 뚜렷하게 관찰할 수 있었으며, 다핵거대세포가 출현하였다. 사이질조직의 부종은 관찰할 수 없었으며, 사이질세포 역시 비교적 온전하게 보존되어 있었다. 따라서 6-AN은 햄스터 정소에서 정자발생세포, 지지세포 등에는 영향을 미치나 사이질세포에는 영향을 주지 않는 것으로 사료된다.

In this study morphological changes of the testes of golden hamsters treated with 6-aminonicotinamide (6-AN, 10 mg/kg body weight) in every two days were investigated using light and transmission electron microscopes. After the 7th injection of 6-AN, body weights of hamsters were significantly reduced, and the weight of testes were markedly reduced in the group of hamsters after 5th injections. Degeneration of seminiferous epithelium appeared first in the group receiving 5th injections, which were followed by severe degenerations after the 9th injection. In the degenerated seminiferous epithelium, deep vacuolization, and destruction of spermatogenic cells and Sertoli cells were also oberved. Multinuclear giant cells were also observed in the lumen of destructed seminiferous epithelium. But there were no edematous changes in the interstitial tissue, and the Leydig cells were found to be relatively intact. Therefore, these results show that 6-AN trigger severe morphological alteration of the spermatogenic cells and Sertoli cells, however Leydig cells are unaltered by 6-AN.

키워드

참고문헌

  1. Aikawa H, Suzuki K: Lesions in the skin, intestine and central nervous system induced by an antimetabolite of niacin. Am J Pathol 122 : 335-342, 1986
  2. Bakalska M, Atanassoba N, Angelova P, Koeva I, Nikolov B, Davidoff M: Degeneration and restoration of spermatogenesis in relation to the changes in Leydig cell population following ethane dimethanesulfonate treatment in adult rats. Endo Regul 35 : 209-215, 2001
  3. Bolin DC, Carlton WW: The effect of 6-aminonicotinamide on testicular development in the rat. Vet Human Toxicol 38(2) : 85-88, 1996
  4. Desphande SS, Albuquerque EX, Kauffman FC, Guth L: Physiological, biochemical and histological changes in skeletal muscles, neuromuscular junction and spinal cord of rats rendered paraplegic by administration of 6-aminonicotinamide. Brain Res 140 : 89-109, 1978 https://doi.org/10.1016/0006-8993(78)90240-8
  5. Formiglin L, Scelsi R, Poggi P, Gregotti C, DiNucci A, Sabboni E, Gotarcli L, Manzo L: Thallium-induced testicular toxicity in the rat. Environm Res 40 : 531-539, 1986 https://doi.org/10.1016/S0013-9351(86)80128-1
  6. Gray LE, Klinefelter G, Kelce W, Laskey J, Ostby J, Ewing L: Hamster Leydig cells are less sensitive to ethane dimethanesulfonate when compared to rat Leydig cells both in vitro and in vivo. Toxicol Appl Pharmacol 130 : 248-256, 1995 https://doi.org/10.1006/taap.1995.1030
  7. Griffiths IR, Kelly PAT, Grome JJ: Glucose utilization in the CNS in the acute gliopathy due to 6-aminonicotinamide. Lab Invest 44(6) : 547-552, 1981
  8. Herken H, Meyer-Estorf G, Halbhubner K, Loos D: Spastic paresis after treatment of 6-aminonicotinamide: Metabolic disorders in the spinal cord and electromyographically recorded changes in the hind limbs of the rats. Naunyn-Schmiederberg' Arch Pharmacol 293 : 245-255, 1976 https://doi.org/10.1007/BF00507347
  9. Horita N, Ishii T, Izumiyama Y: Ultrastructure of 6-aminonico-tinamide induced lesions in the CNS of rats. II. Alterations of the nervous susceptibility with aging. Acta Neuropathol (Berl) 49 : 19-27, 1980 https://doi.org/10.1007/BF00692215
  10. Johnson WT, McColl JD: 6-aminonicotinamide, a potent nicotinamide antagonist. Science 122 : 834, 1955 https://doi.org/10.1126/science.122.3174.834
  11. Kauffman FC, Johnson EC: Cerebral energy reserves and glycolysis in neural tissues of 6-aminonicotinamide treated mice. J Neurobiol 5(5) : 379-392, 1974 https://doi.org/10.1002/neu.480050502
  12. Kim JC, Kim KH, Chung MK: Testicular cytotoxicity of DA-125, a new anthracycline anticancer agent, in rat. Repro Toxicol 13 : 391-397, 1999 https://doi.org/10.1016/S0890-6238(99)00028-3
  13. Kohler E, Barrach HJ, Neubert D: Inhibition of NADP dependent oxidoreductases by the 6-AN analogue of NADP. FEBS Letters 6(3) : 225-228, 1970 https://doi.org/10.1016/0014-5793(70)80063-1
  14. Krinke GJ, Classen W: Spongiform neuropathy induced in dogs by prlonged, low-level administration of 6-aminonicotinamide. Exp Toxic Pathol 50 : 277-282, 1998 https://doi.org/10.1016/S0940-2993(98)80007-9
  15. Mayanil CK, Kazmi SMI, Baous NZ: Effects of 6-AN on monoamine oxidase and NaK ATPase activity in different regions of rat brain. Biochem Pharmacol 33(19) : 3021-3023, 1984 https://doi.org/10.1016/0006-2952(84)90603-8
  16. Politis MJ: 6-Aminonicotinamide selectively causes necrosis in reactive astroglia cells in vivo. Preliminary morphological observations. J Neurosci 92(1) : 71-79, 1989
  17. Sarkander HI, Knoll-Kohler E, Cervos-Navaro J: Repression of glial RNA transcription during the development of 6-aminonicotinamide induced acute gliopathy. J Pharmacol Exp Ther 205(2) : 503-514, 1978
  18. Wolf A, Cowen D: Pathological changes in the central nervous system produced by 6-aminonicotinamide. Bull NY Acad Med 35(12) : 814-817, 1959
  19. Working PK, Bus JS, Hamm TE: Reproductive effects of inhaled methyl chloride in the male Fischer 344 Rat. II. Spermatogonial toxicity and sperm quality. Toxicol Appl Pharmacol 77 : 144-157, 1985 https://doi.org/10.1016/0041-008X(85)90275-3
  20. Yang YC: Ultrastructural changes of the spinal cord after treatment with 6-Aminonicotinamide. Kor J Electron Microscopy 27(3) : 281-293, 1997
  21. Yang YC, Cho BP, Kang HS, Park IK: Morphological changes in the central canal of the hamster spinal cord after treatment with 6-Aminonicotinamide. Kor J Electron Microscopy 27(2) : 177-187, 1997
  22. Yang YC, Kim JY, Park IK: Nuerotoxin 6-aminonicotinamide affects levels of soluble proteins and enzyme activities in various tissues of golden hamsters. IJBCB 32 : 549-556, 2000