Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Effects of 6-aminonicotinamide on the Testes of Golden Hamster

6-Aminonicotinamide가 햄스터의 정소에 미치는 영향

  • Lee, Jin-Suk (Department of Anatomy, Yonsei University, Wonju College of Medicine) ;
  • Choi, Byoung-Young (Department of Anatomy, Yonsei University, Wonju College of Medicine) ;
  • Kim, Dong-Heui (Department of Environmental Medical Biology, Yonsei University, Wonju College of Medicine) ;
  • Jung, Won-Sug (Department of Anatomy, Yonsei University, Wonju College of Medicine) ;
  • Cho, Byung-Pil (Department of Anatomy, Yonsei University, Wonju College of Medicine) ;
  • Yang, Young-Chul (Department of Anatomy, Yonsei University, Wonju College of Medicine)
  • 이진숙 (연세대학교 원주의과대학 해부학교실) ;
  • 최병영 (연세대학교 원주의과대학 해부학교실) ;
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 정원석 (연세대학교 원주의과대학 해부학교실) ;
  • 조병필 (연세대학교 원주의과대학 해부학교실) ;
  • 양영철 (연세대학교 원주의과대학 해부학교실)
  • Published : 2008.09.30
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Abstract

In this study morphological changes of the testes of golden hamsters treated with 6-aminonicotinamide (6-AN, 10 mg/kg body weight) in every two days were investigated using light and transmission electron microscopes. After the 7th injection of 6-AN, body weights of hamsters were significantly reduced, and the weight of testes were markedly reduced in the group of hamsters after 5th injections. Degeneration of seminiferous epithelium appeared first in the group receiving 5th injections, which were followed by severe degenerations after the 9th injection. In the degenerated seminiferous epithelium, deep vacuolization, and destruction of spermatogenic cells and Sertoli cells were also oberved. Multinuclear giant cells were also observed in the lumen of destructed seminiferous epithelium. But there were no edematous changes in the interstitial tissue, and the Leydig cells were found to be relatively intact. Therefore, these results show that 6-AN trigger severe morphological alteration of the spermatogenic cells and Sertoli cells, however Leydig cells are unaltered by 6-AN.

6-aminonicotinamide (6-AN)의 햄스터 정소에 미치는 영향을 확인하기 위해 실험군에는 체중 kg 당 10 mg의 6-AN을, 대조군에는 동량의 생리식염수를 격일로 복강 투여한 후 정소의 변화를 광학 및 투과전자현미경을 이용하여 관찰하였다. 최초 6-AN 투여 당시의 체중에 비해 7회 투여군부터 유의하게 체중이 감소하였으며, 정소 중량의 감소는 5회 투여시 크게 감소하여 이후 비슷한 양상을 보였다. 정세관상피의 퇴행변화는 5회 투여군부터 나타나기 시작하여 9회 투여군부터는 대부분의 정세관이 심하게 손상을 받은 것으로 나타났다. 손상을 받은 정세관에서 정세관상피를 이루는 정자발생세포 및 지지세포 모두 심한 공포화에 따른 세포의 파괴를 뚜렷하게 관찰할 수 있었으며, 다핵거대세포가 출현하였다. 사이질조직의 부종은 관찰할 수 없었으며, 사이질세포 역시 비교적 온전하게 보존되어 있었다. 따라서 6-AN은 햄스터 정소에서 정자발생세포, 지지세포 등에는 영향을 미치나 사이질세포에는 영향을 주지 않는 것으로 사료된다.

Keywords

References

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