Applied Microscopy

  • 제38권3호
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  • Pages.151-157
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  • 2008
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
한국현미경학회 (Korean Society of Microscopy)
권호 표지

흰쥐 삼차신경감각핵에서 $P2X_3$와 TRPV1의 공존에 관한 연구

Coexpression of $P2X_3$ with TRPV1 in the Rat Trigeminal Sensory Nuclei

  • 문용석 (대구가톨릭대학교 의과대학 해부학교실) ;
  • 류창현 (경북대학교 치과대학 구강해부학교실) ;
  • 조이슬 (경북대학교 치과대학 구강해부학교실) ;
  • 김홍태 (대구가톨릭대학교 의과대학 해부학교실) ;
  • 박매자 (경북대학교 의과대학 해부학교실) ;
  • 백상규 (경북대학교 치과대학 구강해부학교실) ;
  • 문제일 (경북대학교 치과대학 구강해부학교실) ;
  • 김윤숙 (경북대학교 치과대학 구강해부학교실) ;
  • 배용철 (경북대학교 치과대학 구강해부학교실)
  • Moon, Yong-Suk (Department of Anatomy, School of Medicine, Catholic University of Daegu) ;
  • Ryoo, Chang-Hyun (Department of Oral Anatomy, School of Dentistry, Kyungpook National University) ;
  • Cho, Yi-Sul (Department of Oral Anatomy, School of Dentistry, Kyungpook National University) ;
  • Kim, Hong-Tae (Department of Anatomy, School of Medicine, Catholic University of Daegu) ;
  • Park, Mae-Ja (Department of Anatomy, School of Medicine, Kyungpook National University) ;
  • Paik, Sang-Kyoo (Department of Oral Anatomy, School of Dentistry, Kyungpook National University) ;
  • Moon, Che-Il (Department of Oral Anatomy, School of Dentistry, Kyungpook National University) ;
  • Kim, Yun-Sook (Department of Oral Anatomy, School of Dentistry, Kyungpook National University) ;
  • Bae, Yong-Chul (Department of Oral Anatomy, School of Dentistry, Kyungpook National University)
  • 발행 : 2008.09.30
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초록

삼차신경계에서 $P2X_3$와 TRPV1 면역양성 일차들신경섬유는 통각정보의 전달에 중요한 역할을 한다. 본 연구에서는 삼차신경절 및 삼차신경꼬리핵에서 $P2X_3$와 TRPV1 면역양성 신경세포의 형태학적 특성 및 투사양식을 이해하기 위하여, 흰쥐 삼차신경절 및 삼차신경꼬리핵에서 $P2X_3$와 TRPV1에 대한 항체를 사용하여 형광면역염색법 및 형태계측학적인 기법을 시행하여 다음과 같은 결과를 얻었다. $P2X_3$ 면역양성 신경세포중 77.4%의 신경세포에서 (1,401/1,810) TRPV1이 동시에 발현되었으며, TRPV1 면역양성 신경세포중 51.9% (1,401/2,698)의 신경세포에서 $P2X_3$와 공존을 보였다. $P2X_3$와 TRPV1에 동시에 면역양성반응을 보이는 신경세포는 중간크기의 신경세포에서 주로 관찰되었으며, $P2X_3$ 혹은 TRPV1 면역양성 신경세포중 아주 작거나 큰 신경세포에서는 공존하지 않았다. 삼차신경꼬리핵에서 $P2X_3$ 면역양성 신경섬유 및 신경종말들은 제1층과 제2층에 분포하는데 주로 제2층의 안쪽부위에서 밀도가 높게 관찰되었으며, TRPV1 면역양성 신경섬유 및 신경종말들은 제1층과 제2층의 바깥쪽에서 밀도가 높게 관찰되었다. $P2X_3$와 TRPV1이 공존하는 신경섬유 및 신경종말들은 제2층의 안쪽과 바깥쪽의 경계부위에서 관찰되었다. 이러한 연구결과는 $P2X_3$와 TRPV1을 동시에 발현하는 신경세포는 구강안면영역에서 통각정보의 처리에 독특한 역할을 수행할 것이라는 것을 시사한다.

Trigeminal primary afferents expressing $P2X_3$ or transient receptor potential vanilloid 1 (TRPV1) are involved in the transmission of nociceptive information. In order to characterize $P2X_3$- and TRPV1-immunopositive neurons in the trigeminal ganglion (TG) and trigeminal caudal nucleus (Vc), we performed immunofluorescence experiments using anti-$P2X_3$ and anti-TRPV1 antisera and a morphometric analysis. 77.4% (1,401/1.801) of all the $P2X_3$-postive neurons coexpressed TRPV1 and 51.9% (1,401/2,698) of all the THFV1-immunopositive neurons also costained for $P2X_3$ in the TG. Immunoreactivity for both $P2X_3$ and TRPV1 were present in medium-sized neurons but not in small- and large-sized neurons. $P2X_3$ and/or TRPV1-immunopositive fibers were observed in the primary afferents and their associated axons in the Vc. These fibers and terminals were distributed in the superficial lamina of Vc: $P2X_3$-immunopositive fibers and terminals were distributed in the lamina I and II, expecially in the inner part of lamina II (lamina IIi), whereas TRPV1-immunopositive ones were densely detected in the lamina I and outer part of lamina II (lamina IIo). Immunopositive fibers and terminals for both $P2X_3$ and TRPV1 were observed on the border between lamina IIi and IIo. These results suggest that terminals coexpressing $P2X_3$ and TRPV1 are involved in specific roles in the transmission and processing of orofacial nociceptive information.

키워드

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