Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Inhibitory Effects of Leachianone A from Sophora fIavescens Ait. against Cadmium Induced Cytotoxicity

고삼으로부터 분리된 Leachianone A의 카드뮴에 대한 독성억제 효과

  • Young, Hee-Tae (Department of Food Nutrition, Daejeon Health Sciences College) ;
  • Choi, Hwa-Jung (Natural Medicine Research Centre, Korea Research Institute of Bioscience & Biotechnology) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 양희태 (대전보건대학 식품영양과) ;
  • 최화정 (생명공학연구원 천연의학연구부) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2008.10.25
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Abstract

In this study, cytotoxicity of cadmium on NIH 3T fibroblasts was utilized in order to discover antitoxic compound in methanol extract of Sophora fIavascens Ait. There were treatment groups; control (medium only), $MTT_{50}$ group and five experimental groups. MTT {3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H- tetrazoliumbromide} assay was performed to evaluate the cytotoxicity of cell organelles and $IC_{50}$ was also measured. Accordingly we have examined the detoxification effects of methanol extract of S. flavescens Ait. and leachianone A (LA) on cadmium-treated NIH 3T3 fibroblasts ($IC_{50}=\;12.5{\mu}M$) to observe morphological changes by the light microscopy. Both S. flavescens Ait. methanol extract and LA showed inhibitory effects on cadmium-induced cytotoxicity. Furthermore, LA showed dose-dependency in detoxication. From these results, it is conceivable to suggest that LA from S. flavescens Ait. methanol extract is a potential antitoxic agent.

Keywords

References

  1. Beattie, J.H., Marion, M., Schmit, J.P., Denizeau, F. The cytotoxic effects of cadmium chloride and mercuric chloride mixtures in rat primary hepatocyte cultures. Toxicology 62: 161-173, 1990 https://doi.org/10.1016/0300-483X(90)90107-R
  2. Williams, P.L., Burson, J.L. Industrial Toxicology.(eds) Van Nostrand Reinhold. pp 197-210, 1985
  3. Foulkes E.C. (eds) Biological Roles of Metallothionein, Elsevier, New York, pp 215-222, 1982
  4. Vallee, B.L., Ulmer, D.D. Biochemical effects of mercury, cadmium and lead. Ann. Rev. Biochem. 4: 91-128, 1972
  5. 국립보건원. 식품중의 미량금속에 관한 조사연구. 29: 365- 377, 1992
  6. 김운성, 이철호, 김성조, 이주돈, 문광현, 백승화. 알로에 첨가식이 흰쥐의 카드뮴 독성에 미치는 영향. 한국식품과학회지 27: 555-563, 1995
  7. 최성인, 이정희, 이서래. 동물실험에 의한 녹차음료의 카드뮴 및 납 제거효과. 한국식품과학회지 26: 745-749, 1994
  8. Eiinder, C.G. Cadmium: Uses, Occurrence and Intake. Cadmium and Health. L. Friberg, et al, Eds. CRC Press, Bca Raton, FL. 1985
  9. WHO. Cadmium in Recommended Health-Based Limits in Occupational Health to Heavy Metals. WHO Techanical Report Series, pp 647-655, 1980
  10. WHO. Health hazard of the Human Environmental. Geneva, WHO, pp 35-37, 1977
  11. Friberg, L., Kjellstrom, T., Nordberg, G. Cadmium. Handbook on the Toxicology of Metals. Elsevier/ North- Holland Biomedical Press, New York, pp 130-184, 1986
  12. Elinder, C.G. Cadmium : Wses, Occurrence and Intake. Cadmium and Health. L. Friberg, et al, Eds. CRC Press, Boca Raton, FL. 1985
  13. Kim, M.J., Rhee, S.J. Effects of Korean green tea, colong tea and black tea beverage on the removal of cadmium in rat. J. Korean Soc. Food Nutr. 23: 784-791, 1994
  14. Sakanaka, S., Kim, M., Taniguchi, M., Yamamoto, T. Antibacterial substances in Japanese green tea extract against Streptococcus mutans. a Cariognic bacterium. Argic. Biol. Chem. 53: 2307-2311, 1989 https://doi.org/10.1271/bbb1961.53.2307
  15. Muramatsu, K., Fukuyo, M,, Jara, Y. Efect of green tea catechins on plasma cholestero level in cholesterol fed rats. J. Nutr. Sci. Vitaminol. 32: 613-612, 1986 https://doi.org/10.3177/jnsv.32.613
  16. Cheng, S.J. The preliminary study of inhibitory effects of green tea antioxidant on mutation. Acta Exp. Biol. 9: 328-334, 1986
  17. Sakanaka, S., Shimura, N., Aizawa, M., Kim, M., Yamamoto, T. Preventive effect of green tea polyphenols against dental caries in conventional rats. Biosci. Biotech. Biochem. 56: 592-594, 1992 https://doi.org/10.1271/bbb.56.592
  18. Hayashi, E., Hayashi, M., Yamazoe, H. Pharmacological action of tea extract on the central nervous system in mice. Oyo Yalari 40: 351-356, 1990
  19. Matsuzaki, T., Hata, Y. Antioxidative activity of tea leaf catechins. Nippon Nogeikagaku Kaish 59: 129-134, 1985 https://doi.org/10.1271/nogeikagaku1924.59.129
  20. Choi, J.H., Rhee, S.J. Effects of green tea catechin on cadmium accumulation in chronic cadmium poisoned rats. Korean J. Nutr. 34: 384-392, 2001
  21. 신민교. 임상본초학, 남산당, 서울. pp 314-315, 1996
  22. Yang, H.T., Choi, H.J., Baek, S.H. Antimicrobial effect of flavanones from Sophora flavescens Ait. J. Pharm. Soc. Korea Accepted, 2008
  23. Mosmann, T.J. Rapid colorimetric assays for cellular growth and survial; application of proloferation and cytotoxicity assays, J. Immunol. Methods 65: 55-63, 1983 https://doi.org/10.1016/0022-1759(83)90303-4
  24. Kim, H.J., Bea, K.H., Lee, H.J., Eun, J.B., Kim, M.K. Effect of hesperidin extracted from tangerine peel on Cd and lipid metabolism, and antioxidative capacity in rats. Korean J. Nutr. 32: 137-149, 1999
  25. Cornard, J.P., Dangleterre, L., Lapouge, C. Computational and spectroscopic characterization of the molecular and electronic structure of the Pb (II)-quercetin complex. J. Phys. Chem. 109: 10044-10051, 2005 https://doi.org/10.1021/jp053506i
  26. 이정호. 어성초로부터 활성성분의 분리와 약리효과. 원광대학교 한의전문대학원 박사학위 논문. 2002
  27. Baek, S.H., Lee, H., Pae, H.O., Kim, Y.O., Kwak, J.S., Yoo, Y.H., Han, D.S. Delvelopment of antitoxic agents from Korean medicinal plants. Part 5. antitoxic effects of binding of caffeic acid and cadmium on cultured rat neuroglial cells. Kor. J. Toxicol. 11: 241-246, 1995