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Screening of a Natural Feed Additive Having Anti-viral Activity against Influenza A/H5N1

안전한 닭고기 생산을 위한 고병원성 조류인플루엔자 A/H5N1에 항바이러스 효과를 가진 천연 사료첨가제의 탐색

  • Lee, Jang-Hyun (Division of Animal Life Science, Konkuk University) ;
  • Kwon, Su-Min (Division of Animal Life Science, Konkuk University) ;
  • Seo, Sang-Heui (College of Veterinary Medicin,e Chungnam National University) ;
  • Park, Young-Seo (Department of Food Science and Biotechnology, Kyungwon University) ;
  • Kim, Young-Bong (Division of Animal Life Science, Konkuk University) ;
  • Kim, Soo-Ki (Division of Animal Life Science, Konkuk University) ;
  • Paik, Hyun-Dong (Division of Animal Life Science, Konkuk University)
  • 이장현 (건국대학교 동물생명과학부) ;
  • 권수민 (건국대학교 동물생명과학부) ;
  • 서상희 (충남대학교 수의과대학) ;
  • 박영서 (경원대학교 식품생물공학과) ;
  • 김영봉 (건국대학교 동물생명과학부) ;
  • 김수기 (건국대학교 동물생명과학부) ;
  • 백현동 (건국대학교 동물생명과학부)
  • Published : 2008.10.31

Abstract

To search for anit-H5N1 influenza virus agent, the anti-viral activity of methanol and aqueous extracts from thirty medicinal plants were examined in this study. The plant material (30 g) was extracted with methanol (300 mL) for 24 hr at room temperature. Methanol extracts were filtered and evaporated, then freeze-dried. Aqueous extracts were prepared with dried plant material (30 g) and hot distilled water (300 mL). After 3 hr, the aqueous extracts were filtered and evaporated, then lyophilized. Extracts prepared from different plants were tested the antiviral activity against influenza viruses [A/vietnam/1194/04 (H5N1)-NIBRG-14] using the hemagglutination (HA) assay. Among the test plants, Asarum sieboldii was found to be a potent inhibitor of H5N1 influenza virus in MDCK cell culture. Virus titers were 7 log, whereas with methanol extract of Asarum sieboldii for 48 hr titers were 3 log, indicating that methanol extract of Asarum sieboldii inhibited the H5N1 influenza viruses from the infected cells.

Keywords

References

  1. Amonsin, A., Chutinimitkul, S., Pariyothorn, N., Songserm, T., Damrongwantanapokin, S., Puranaveja, S., Jam-on, R., Sae-Heng, N., Payungporn, S., Theamboonlers, A., Chaisingh, A. Tantilertcharoen, R., Suradhat, S., Thanawongnuwech, R., and Poovorawan, Y. (2006) Genetic characterization of influenza A viruses (H5N1) isolated from 3rd wave of Thailand AI outbreaks. Virus Res. 122, 194-199 https://doi.org/10.1016/j.virusres.2006.06.016
  2. Cho, E. and Yoon, S. (1999) Protective effect of Asiasari Radix on rat liver. J. Korean Soc. Hyg. Sci. 5, 85-91
  3. De Jong, M. D., and Hien, T. T. (2006) Avian influenza A (H5N1). J. Clin. Virol. 35, 2-13 https://doi.org/10.1016/j.jcv.2005.09.002
  4. Ehrhardt, C., Hrincius, E. R., Korte, V., Mazur, I., Droebner, K., Poetter, A., Dreschers, S., Schmolke, M., Planz, O., and Ludwig, S. (2007) A polyphenol rich plant extract, CYSTUS052, exerts anti influenza virus activity in cell culture without toxic side effects or the tendency to induce viral resistance. Antiviral Res. 76, 38-47 https://doi.org/10.1016/j.antiviral.2007.05.002
  5. He, G., Qiao, J., Dong, C., He, C., Zhao, L., and Tian, Y. (2008) Amantadine-resistance among H5N1 avian influenza viruses isolated in Northern China. Antiviral Res. 77, 72-76 https://doi.org/10.1016/j.antiviral.2007.08.007
  6. Kim, H., Bang, C., Choi, Y., and Lee, J. (2007) Antioxidant and antiproliferative activities of methanol extracts from leafy vegetables consumed in Korea. Food Sci. Biotechnol. 16, 802-806
  7. Kim, S., Zhang, C. G., and Lim, J. T. (2003) Mechanism of anti-nociceptive effects of Asarum sieboldii Miq. Radix: potential role of bradykinin, histamine and opioid receptormediated pathways. J. Ethnopharmacol. 88, 5-9 https://doi.org/10.1016/S0378-8741(03)00181-8
  8. Le, Q. M., Kiso, M., Someya, K., Sakai, Y. T., Nguyen, T. H., Nguyen, K. H. L., Pham, N. D., Ngyen, H. H., Yamada S., Muramoto, Y., Horimoto, T., Takada, A., Goto, H., Suzuki, T., Suzuki, Y., and Kawaoka Y. (2005) Isolation of drugresistant H5N1 virus. Nature 437, 1108 https://doi.org/10.1038/4371108a
  9. Lee, J. Y., Moon, S. S., and Hwang, B. K. (2005) Isolation and antifungal activity of kakuol, a propiophenone derivative from Asarum sieboldii rhizome. Pest Manag. Sci. 61, 821-825 https://doi.org/10.1002/ps.1050
  10. Mukhtar, M., Arshad, M., Ahmad, M., Pomerantz, R. J., Wigdahl, B., and Parveen, Z. (2008) Antiviral potentials of medicinal plants. Virus Res. 131, 111-120 https://doi.org/10.1016/j.virusres.2007.09.008
  11. Park, K. J. (2003) Evaluation of in vitro antiviral activity in methanol extracts against influenza virus type A from Korean medicinal plants. Phytother. Res. 17, 1059-1063 https://doi.org/10.1002/ptr.1330
  12. Rajput, Z. I., Xiao, C., Hu, S., Arijo, A. G., and Soomro, N. M. (2007) Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS). J. Zhejiang Univ. Sci. B 8, 331-337 https://doi.org/10.1631/jzus.2007.B0331
  13. Seo, S. H., Hoffmann, E., and Webster, R. G. (2004) The NS1 gene of H5N1 influenza viruses circumvents the host antiviral cytokine responses. Virus Res. 103, 107-113 https://doi.org/10.1016/j.virusres.2004.02.022
  14. Shin, S. R., Hong, J. Y., and Yoon, K. Y. (2008) Antioxidant properties and total phenolic contents of cherry elaeagnus (Elaeagnus multiflora Thumb.) leaf extracts. Food Sci. Biotechnol. 17, 608-612
  15. Shortridge, K. F., Zhou, N. N., Guan, Y., Gao, P., Ito, T., Kawaoka, Y., Kodihalli, S., Krauss, S., Markwell, D., Murti, K. G., Norwood, M., Senne, D., Sims, L., Takada, A., and Webster, R. G. (1998) Characterization of avian H5N1 influenza viruses from poultry in Hong Kong. Virology 252, 331-342 https://doi.org/10.1006/viro.1998.9488
  16. Smith, G. J. D., Naipospos, T. S. P., Nguyen, T. D., De Jong, M. D., Vijaykrishna, D., Usman, T. B., Hassan, S. S., Nguyen, T. V., Dao, T. V., Bui, N. A., Leung, Y. H. C., Cheung, C. L., Rayner, J. M., Zhang, J. X., Zhang, L. J., Poon, L. L. M., Li, K. S., Nguyen, V. C., Hien, T. T., Farrar, J., Webster, R. G., Chen, H., Peiris, J. S. M., and Guan, Y. (2006) Evolution and adaptation of H5N1 influenza virus in avian and human hosts in Indonesia and Vietnam. Virology 350, 258-268 https://doi.org/10.1016/j.virol.2006.03.048
  17. Song, J., Lee, K., and Seong, B. (2005) Antiviral effect of catechins in green tea on influenza virus. Antiviral Res.68, 66-74 https://doi.org/10.1016/j.antiviral.2005.06.010
  18. Wang, X., Jia, W., Zhao, A., and Wang, X. (2006) Anti-influenza agents from plants and traditional Chinese medicine. Phytother. Res. 20, 335-341 https://doi.org/10.1002/ptr.1892
  19. Yan, J., Lu, Y., Mao, H., Feng, Y., Xu, C., Shi, W., Weng, J., Li, M., Gong, L., Ge, Q., Zhou, M., Li, Z., and Chen, Y. (2007) Pathogenic and molecular characterization of the H5N1 avian influenza virus isolated from the first human case in Zhejiang province, China. Diagn. Microbiol. Infect. Dis. 58, 399-405 https://doi.org/10.1016/j.diagmicrobio.2007.03.004
  20. Yu, H., Seo, S., Hur, J., Lee, H., Lee, Y., and You, Y. (2006) Asarum sieboldii extracts attenuate growth, acid production, adhesion, and water-insoluble glucan synthesis of Streptococcus mutans. J. Med. Food 9, 505-509 https://doi.org/10.1089/jmf.2006.9.505

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