Changes of Protopanaxadiol Ginsenosides in Ginseng Leaves by Far Infrared and Steaming Heat Treatments

원적외선 및 증숙 처리에 따른 인삼 잎의 Protopanaxadiol Ginsenosides 변화

  • Eom, Seok-Hyun (Hongcheon Institute of Medicinal Herb, Kangwon National University) ;
  • Seo, Su-Hyun (Department of Applied BioScience, KonKuk University) ;
  • Gimery, Amal Kumal (Plant Biotechnology, Bioscience and Biotechnology, Kangwon National University) ;
  • Jin, Cheng Wu (Plant Biotechnology, Bioscience and Biotechnology, Kangwon National University) ;
  • Kango, Eun-Young (Department of Applied BioScience, KonKuk University) ;
  • Kang, Wie-Soo (Plant Biotechnology, Bioscience and Biotechnology, Kangwon National University) ;
  • Chung, Ill-Min (Department of Applied BioScience, KonKuk University) ;
  • Cho, Dong-Ha (Plant Biotechnology, Bioscience and Biotechnology, Kangwon National University)
  • Published : 2008.10.30

Abstract

PPD ginsenosides in ginseng leaf were analyzed to determine effects of either FIR heat or steaming heat treatment. Among the PPD ginsenosides, Rb1, Rc and Rb3 forming four glycoside-attached aglycons were increased as FIR heat temperatures were increased from 60 to $120^{\circ}C$, while Rb3 was decreased. In addition, FIR heat treatment was effective to increase Rd forming a three glycoside-attached aglycon. Rg3 and Rh2 were not increased by the FIR heat treatment. In steaming heat treatment, Rb1 was significantly decreased, while Rb2 was increased. Rd was also increased by increased steaming temperature, yet its content was lower than in the FIR heat treatment. However, the steaming heat treatment increased yields of Rg3 and Rh2, which were not observed in the FIR heat treatment. Thus, FIR heat treatment was beneficial to efficient products of Rb1, Rc, Rb3 and Rd. Steaming heat treatment was effective to higher collection of Rb2, Rg3 and Rh2.

Keywords

References

  1. Cheng LQ, Kim MK, Lee JW, Lee YJ, Yang DC (2006) Conversion of major ginsenoside Rb sub(1) to ginsenoside F sub(2) by Caulobecter leidyia. Biotechnol. Lett. 28(14):1121-1127 https://doi.org/10.1007/s10529-006-9059-x
  2. Eom SH, Jin CW, Park HJ, Kim EH, Chung IM, Kim MJ, Yu CY, Cho DH (2007) Far infrared ray irradiation stimulates antioxidant activity in Vitis flexuosa THUNB. Berries. Korean J. Medicinal Crop Sci. 15(5):319-323
  3. Eom SH, Park HJ, Jin CW, Kim DW, Seo DW, Jeong YH, Cho DH (2008) Changes in antioxidant activity with temperature and time in Chrysanthemum indicum L. teas during elution processes in hot water. J. Food Sci. Biotech. 17(2):408-412
  4. Han BH, Park MH, Han YN, Woo LK, Sankawa U, Yahara S, Tanaka O (1982) Degradation of ginseng saponins under mild acidic conditions. Planta Med. 44(3):146-149 https://doi.org/10.1055/s-2007-971425
  5. Jo JS, Won JY (1995) Safety of the herbicide fluazifon-butyl application on the Korean ginseng (Panax ginseng C.A. Meyer). Korean J. Medicinal Crop Sci. 3(2):146-150
  6. Kim KT, Yoo KM, Lee JW, Eom SH, Hwang IK, Lee CY (2007) Protective effect of steamed American ginseng (Panax quinquefolius L.) on V79-4 cells induced by oxidative stress. J. Ethnopharmacol. 111: 443-450 https://doi.org/10.1016/j.jep.2007.01.004
  7. Li TSC, Mazza G, Cottrell AC, Gao L (1996) Ginsenosides in roots and leaves of American ginseng. J. Agric. Food Chem. 44(3):717-720 https://doi.org/10.1021/jf950309f
  8. Popovich DG, Kitts DD (2004) Generation of ginsenosides Rg3 and Rh2 from North American ginseng. Phytochem. 65(3):337-344 https://doi.org/10.1016/j.phytochem.2003.11.020
  9. Shi W, Wang Y, Li J, Zhang H, Ding L (2007) Investigation of ginsenosides in different parts and ages of Panax ginseng. Food Chem. 102:664-668 https://doi.org/10.1016/j.foodchem.2006.05.053
  10. Tanaka O, Kasai R. In: Herz W, Grisebach H, Kirby GW, Tamm CH Editors (1984) Progress in the chemistry of organic natural products. Springer, New York. Vol. 46 p. 1
  11. Wang CZ, Wu JA, McEntee E, Yuan CS (2006) Saponins composition in American ginseng leaf and berry assayed by high-performance liquid chromatography. J. Agric. Food Chem. 54(6):2261-2266 https://doi.org/10.1021/jf052993w