한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제16권5호
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  • Pages.313-319
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  • 2008
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

반지련 (Scutellaria barbata D. Don) 추출물이 lipopolysaccharide에 의해 활성화된 대식세포에 미치는 영향

Effect of Hot Water Extract from Scutellaria barbata on the Macrophages Activated by Lipopolysaccharide

  • 센팅 (강원대학교 BT학부대학 생명공학부) ;
  • 이용진 ((재) 춘천바이오산업진흥원) ;
  • 조재열 (강원대학교 BT학부대학 생명공학부)
  • Shen, Ting (School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Yong-Jin (Laboratory of functional food development, Chuncheon Bioindustry Foundation) ;
  • Cho, Jae-Youl (School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University)
  • 발행 : 2008.10.30
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초록

S. barbata의 열수 추출물 (Sb-HWE)은 대표적인 염증과정인 LPS에 의해 활성화된 대식세포로 부터의 NO생성, LPS 매개에 의한 세포사멸작용, 및 FITC-dextran의 대식세포내 탐식작용을 매우 효과적으로 억제하였다. 그러나 본 추출물은 SNP로 유도된 라디칼 소거능은 매우 미약한 것으로 나타났다. NF-${\kappa}B$-매개에 의한 루시퍼라제 활성, 및 NF-${\kappa}B$ 활성 관련 신호전달 단백질 (Akt 및 $I{\kappa}B{\alpha}$)에 대한 저해작용은 관찰되지 않은 것으로 보아 이들 추출물의 대식세포 면역반응 조절 기전은 기존의 알려진 방법과는 다른 기전에 의해 진행되는 것으로 판단된다.

Scutellaria barbata was examined to evaluate its modulatory effects on the functional activation of macrophages under lipopolysaccharide (LPS) treatment. To do this, hot water extract (Sb-HWE) was prepared from Scutellaria barbata and several inflammatory parameters such as nitric oxide (NO) production, phagocytosis, reactive oxygen species (ROS) determination and intracellular signaling pathway were selected to be tested. Sb-HWE strongly blocked NO production in LPS-activated RAW264.7 cells in a dose-dependent manner. However, it did not suppress inducible NO synthase (iNOS). In agreement, Sb-HWE did not diminish inflammatory signaling composed of NF-${\kappa}B$ and its upstream activation signaling enzymes such as Akt and $I{\kappa}B{\alpha}$. Sb-HWE protected RAW264.7 cells from LPS-induced cytotoxicity up to 80% at 400\;{\mu}g/ml$. Furthermore, this extract blocked phagocytic uptake of FITC-dextran, while sodium nitroprusside (SNP)-induced ROS generation in RAW264.7 cells was not decreased. Therefore, our data suggest that Sb-HWE may have differential immunoregulatory function depending on macrophage-mediated immune responses.

키워드

참고문헌

  1. Brune B, Zhou J, von Knethen A (2003) Nitric oxide, oxidative stress, and apoptosis. Kidney Int. Suppl. 84:S22-24
  2. Chen F, Shi X (2002) NF-kappaB, a pivotal transcription factor in silica-induced diseases. Mol. Cell. Biochem. 234-235(1-2):169-176 https://doi.org/10.1023/A:1015915000265
  3. Chen LG, Hung LY, Tsai KW, Pan YS, Tsai YD, Li YZ, Liu YW (2008) Wogonin, a bioactive flavonoid in herbal tea, inhibits inflammatory cyclooxygenase-2 gene expression in human lung epithelial cancer cells. Mol. Nutr. Food Res. May 21. [Epub ahead of print]
  4. Cho JY (2007) Inhibitory effect of extracts from woody plants on tumor necrosis factor-alpha production in lipopolysaccharide-stimulated RAW264.7 cells. Korean J. Medicinal Crop. Sci. 15(4):271-275
  5. Cho JY, Park J, Kim PS, Yoo ES, Baik KU, Park MH (2001) Savinin, a lignan from Pterocarpus santalinus inhibits tumor necrosis factor-alpha production and T cell proliferation. Biol. Pharm. Bull. 24(2):167-171 https://doi.org/10.1248/bpb.24.167
  6. Dai SJ, Liang DD, Ren Y, Liu K, Shen L (2008) New neoclerodane diterpenoid alkaloids from Scutellaria barbata with cytotoxic activities. Chem. Pharm. Bull. (Tokyo) 56(2):207-209 https://doi.org/10.1248/cpb.56.207
  7. Dai SJ, Wang GF, Chen M, Liu K, Shen L (2007) Five new neo-clerodane diterpenoid alkaloids from Scutellaria barbata with cytotoxic activities. Chem. Pharm. Bull. (Tokyo) 55(8):1218-1221 https://doi.org/10.1248/cpb.55.1218
  8. Dijkstra G, Moshage H, Jansen PL (2002) Blockade of NF-kappaB activation and donation of nitric oxide: new treatment options in inflammatory bowel disease? Scand. J. Gastroenterol. Suppl. 236:37-41
  9. Edwards JP, Zhang X, Frauwirth KA, Mosser DM (2006) Biochemical and functional characterization of three activated macrophage populations. J. Leukoc. Biol. 80(6):1298-1307 https://doi.org/10.1189/jlb.0406249
  10. Fang H, Pengal RA, Cao X, Ganesan LP, Wewers MD, Marsh CB, Tridandapani S (2004) Lipopolysaccharide-induced macrophage inflammatory response is regulated by SHIP. J. Immunol. 173(1):360-366 https://doi.org/10.4049/jimmunol.173.1.360
  11. Forman HJ, Torres M (2001) Signaling by the respiratory burst in macrophages. IUBMB Life 51(6):365-371 https://doi.org/10.1080/152165401753366122
  12. Jiangsu New Medical College (1997). Dictionary of Chinese Materia Medica. Science and Technology Press of Shanghai, Shanghai., p. 343
  13. Katiyar SK, Matsui MS, Elmets CA, Mukhtar H (1999) Polyphenolic antioxidant (-)-epigallocatechin-3-gallate from green tea reduces UVB-induced inflammatory responses and infiltration of leukocytes in human skin. Photochem. Photobiol. 69(2):148-153
  14. Lee SK, Kim HS, Lee HJ, Lee J, Jeon BH, Jun CD, Lee SK, Kim EC (2006) Dual effect of nitric oxide in immortalized and malignant human oral keratinocytes: induction of apoptosis and differentiation. J. Oral. Pathol. Med. 35(6):352-360 https://doi.org/10.1111/j.1600-0714.2006.00439.x
  15. Lee YG, Byeon SE, Kim JY, Lee JY, Rhee MH, Hong S, Wu JC, Lee HS, Kim MJ, Cho DH, Cho JY (2007a) Immunomodulatory effect of Hibiscus cannabinus extract on macrophage functions. J. Ethnopharmacol. 113(1):62-71 https://doi.org/10.1016/j.jep.2007.04.019
  16. Lee YG, Cho JY (2007) Inhibitory effect of curcumin on nitric oxide production in lipopolysaccharide-stimulated RAW264.7 cells. Korean J. Medicinal Crop. Sci. 15(4):271-275
  17. Lee YG, Kim JY, Lee JY, Byeon SE, Hong EK, Lee J, Rhee MH, Park HJ, Cho JY (2007b) Regulatory effects of Codonopsis lanceolata on macrophage-mediated immune responses. J. Ethnopharmacol. 112(1):180-188 https://doi.org/10.1016/j.jep.2007.02.026
  18. Lee YG, Lee WM, Kim JY, Lee JY, Lee IK, Yun BS, Rhee MH, Cho JY (2008) Src kinase-targeted anti-inflammatory activity of davallialactone from Inonotus xeranticus in lipopolysaccharide-activated RAW264.7 cells. Br. J. Pharmacol. 154(4):852-863 https://doi.org/10.1038/bjp.2008.136
  19. Lin HY, Shen SC, Chen YC (2005) Anti-inflammatory effect of heme oxygenase 1: glycosylation and nitric oxide inhibition in macrophages. J. Cell. Physiol. 202(2):579-590 https://doi.org/10.1002/jcp.20160
  20. Lin YS, Hsieh M, Lee YJ, Liu KL, Lin TH (2008) AH23848 accelerates inducible nitric oxide synthase degradation through attenuation of cAMP signaling in glomerular mesangial cells. Nitric Oxide 18(2):93-104 https://doi.org/10.1016/j.niox.2007.10.005
  21. Maiorana A, O'Driscoll G, Taylor R, Green D (2003) Exercise and the nitric oxide vasodilator system. Sports Med. 33(14):1013-1035 https://doi.org/10.2165/00007256-200333140-00001
  22. Migliaccio E, Giorgio M, Pelicci PG (2006) Apoptosis and aging: role of p66Shc redox protein. Antioxid. Redox Signal. 8(3-4):600-608 https://doi.org/10.1089/ars.2006.8.600
  23. Sato Y, Suzaki S, Nishikawa T, Kihara M, Shibata H, Higuti T (2000) Phytochemical flavones isolated from Scutellaria barbata and antibacterial activity against methicillin-resistant Staphylococcus aureus. J. Ethnopharmacol. 72(3):483-488 https://doi.org/10.1016/S0378-8741(00)00265-8
  24. Savina A, Amigorena S (2007) Phagocytosis and antigen presentation in dendritic cells. Immunol. Rev. 219(1):143-156 https://doi.org/10.1111/j.1600-065X.2007.00552.x
  25. Suh SJ, Yoon JW, Lee TK, Jin UH, Kim SL, Kim MS, Kwon DY, Lee YC, Kim CH (2007) Chemoprevention of Scutellaria bardata on human cancer cells and tumorigenesis in skin cancer. Phytother. Res. 21(2):135-141 https://doi.org/10.1002/ptr.2010
  26. Taylor EL, Megson IL, Haslett C, Rossi AG (2003) Nitric oxide: a key regulator of myeloid inflammatory cell apoptosis. Cell Death. Differ. 10(4):418-430 https://doi.org/10.1038/sj.cdd.4401152
  27. Tetley TD (2005) Inflammatory cells and chronic obstructive pulmonary disease. Curr. Drug Targets Inflamm. Allergy 4(6):607-618 https://doi.org/10.2174/156801005774912824