Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Hypolipidemic and Antioxidant Effects to Ginseng Extract (PD：PT = 1) in Apo E Null Mice

Apo E Null mice에서 인삼 Ginsenosides (PD：PT = 1) 추출물의 지질개선 및 항산화 효과

  • 장수정 (성신여자대학교 식품영양학과) ;
  • 김성수 (한국식품연구원) ;
  • 이명숙 (성신여자대학교 식품영양학과)
  • Published : 2008.10.31
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Abstract

Panax ginseng C.A. Meyer (Panax ginseng) has been used for several thousand years to prolong longevity in Asian countries. Ginsenosides are the most active components isolated from ginseng and belong to damarane saponin which are separated into protopanaxadiol and protopanaxtriol. To evaluate the complex effect of ginsenoside in apo E null mice, ginseng extract were intraperioneally (i.p.) injected and provided high-cholesterol diet for 12 weeks. Ginseng extract came from were i.p. injected with dose of 100 mg/kg/day for 4 weeks in the last experimental duration. Ginseng extract used experiment was abundant Rb1, Rc, Re, and Rg1 and PD：PT ratio was 1.2. The high-cholesterol diet induced liver damage was significantly reduced by ginseng extract. Results from plasma lipid profiles and atherogenic index were improved by ginseng extracts. The GE group significantly decreased plasma TG and TC by 73% and 61% compared to apo E (-/-) group. Also ginseng extract tend to decrease lipid profiles and lipidperoxidation contents in liver and heart. Ginseng extract with an abundant amount of Rg1 significantly suppressed the apoptosis induction of cardiac tissue. In conclusion, ginseng extract (PD：PT = 1) was improved lipid profiles and anti-oxidant effects.

Keywords

References

  1. Ancion A, Lancellortti P, Prerard LA. Congestive heart failure and diabetes mellitus. Rev Med Liege 2005; 60 (5-6): 536-540
  2. Liu CX, Xiao PG. Recent advances on ginseng research in China. J Ethnopharmacol 1992; 36 (1): 27-38 https://doi.org/10.1016/0378-8741(92)90057-X
  3. Xie JT, Mehendale SR, Li X, Quigg R, Wang X, Wang CZ, Wu JA, Aung HH, Rue PA, Bell GI, Yuan CS. Anti-diabetic effect of ginsenoside Re in ob/ob mice. Biochimica et Biophysica Acta 2005; 1740 (3): 319-325 https://doi.org/10.1016/j.bbadis.2004.10.010
  4. Mayr M, Mayr U, Chung YL, Yin X, Griffiths JR, Xu Q. Vascular proteomics: linking proteomic and metabolomic changes. Proteomics 2004; 4 (12): 3751-3761 https://doi.org/10.1002/pmic.200400947
  5. Jung NP, Jin SH. Studies on the physiological and biochemical effects of Korean ginseng. Korean J Ginseng Sci 1996; 20 (4): 431-471
  6. Kim SS, Park HY, Byun YH, Hwang BG, Lee JH. The effects on the blood lipid profiles and body fat by long term administration of red ginseng product. J Ginseng Res 2002; 26 (2): 67-73 https://doi.org/10.5142/JGR.2002.26.2.067
  7. Sengupta S, Toh SA, Sellers LA, Skepper JN, Koolwijk P, Leung HW, Yeung HW, Wong RNS, Sasisekharan R, Fan TPD. Modulating angiogenesis: the yin and the yang in ginseng. Circulation 2004; 110 (10): 1219-1225 https://doi.org/10.1161/01.CIR.0000140676.88412.CF
  8. Sievenpiper JL, Arnason JT, Leiter LA, Vuksan V. Decreasing, null and increasing effects of eight popular types of ginseng on acute postprandial glycemic indices in healthy humans: the role of ginsenosides. J Am Coll Nutr 2004; 23 (3): 248-258 https://doi.org/10.1080/07315724.2004.10719368
  9. Kim YS, Kim DS, Kim SI. Ginsenoside Rh2 and Rh3 induce differentiation of HL-60 cells into granulocytes: modulation of protein kinase C isoforms during differentiation by ginsenoside Rh2. Int J Biochem Cell Biol 1998; 30 (3): 327-338 https://doi.org/10.1016/S1357-2725(97)00141-6
  10. Wakabayashi C, Murakami K, Hasegawa H, Murata J and Saiki I. An intestinal bacterial metabolite of ginseng protopanaxadiol saponins has the ability to induce apoptosis in tumor cells. Biochem Biophys Res Commun 1998; 246 (3): 725-730 https://doi.org/10.1006/bbrc.1998.8690
  11. Liu CX, Xiao PG. Recent advances on ginseng research in China. J Ethnopharmacol 1992; 36 (1): 27-38 https://doi.org/10.1016/0378-8741(92)90057-X
  12. Noh YH, Lim GR, Koo JH. Comparison of the effects of ginseng total saponin, ginsenoside-Rb1, ginsenoside-Rb2 and lovastatin on the expression of mRNAs for HMG CoA reductase and LDL receptor. Korean J Ginseng Sci 1996; 20 (3): 241-247
  13. Kim MS, Lee MS, Kim SH, Kim SH, Kim HJ, Sung MJ, Kim HY, Kwon DY, Hwang JT. Anti-obesity effects of ginsenoside Rd via AMPK and PPAR gamma. Korean J Biotechnol Bioeng 2007; 22 (5): 341-344
  14. Zhang JT. Improvement of learning and memory and biphasic regulation of body temperature in mice induced by ginsenoside Rg1 and Rb. Proceedings of the 4th of China-Japan Symposium on Drug. Aug; 1991
  15. Zhang SH, Reddick RL, Piedrahita JA, and Maeda N. Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein E. Science 1992; 258 (5081): 468-471 https://doi.org/10.1126/science.1411543
  16. Guo Y, Zhang C, Du X, Nair U, Yoo TJ. Morphological and functional alterations of the cochlea in apolipoprotein E gene deficient mice. Hear Res 2005; 208 (1-2): 54-67 https://doi.org/10.1016/j.heares.2005.05.010
  17. Shibata S, Tanaka O, Ando T, Sado M, Tsushima S, Ohsawa T. Chemical studies on oriental plant drugs. XIV. Protopanaxadiol, a genuine sapogenin of ginseng saponins. Chem Pharm Bull (Tokyo) 1966; 14 (6): 595-600 https://doi.org/10.1248/cpb.14.595
  18. Vassault A, Sebille L, Bailly M. Evaluation according to the NCCLS protocol of the Kodak-Ektachem procedure applied to the estimation of glucose and urea in plasma (author's transl). Ann Biol Clin (Paris) 1981; 39 (1): 17-28
  19. Gordon T, Castelli WP, Hjortland MC, Kannel WB, Dawber TR. High density lipoprotein as a protective factor against coronary heart disease. The Framingham Study. Am J Med 1977; 62 (5): 707-714 https://doi.org/10.1016/0002-9343(77)90874-9
  20. Lee MS, Bae MA. Docosahexaenoic acid induces apoptosis in CYP2E1-containing HepG2 cells by activating the c-Jun N-terminal protein kinase related mitochondrial damage. J Nutr Biochem 2007; 18 (5): 348-354 https://doi.org/10.1016/j.jnutbio.2006.06.003
  21. Ancion A, Lancellortti P, Prerard LA. Congestive heart failure and diabetes mellitus. Rev Med Liege 2005; 60 (5-6): 536-540
  22. Yoon SH, Park EJ, Oh KH, Chung YG, Kwon OJ. The effect of lithosperomi radixon benzo(a)pyrene-induced hepatotoxicity. J Korean Soc Food Sci Nutr 1993; 22 (2): 144-148
  23. Gum SI, Jo SJ, Ahn SH, Kim SG, Kim JT, Shin HM, Cho MK. The potent protective effect of wild ginseng (Panax ginseng C.A. Meyer) against benzo$[\alpha]$pyrene-induced toxicity through metabolic regulation of CYP1A1 and GSTs the potent protective effect of wild ginseng. J Ethnopharmacol 2007; 112 (3): 568-576 https://doi.org/10.1016/j.jep.2007.05.014
  24. Lee HU, Bae EA, Han MJ, Jae N, Kim DH. Hepatoprotective effect of ginsenside Rb1 and compound K on tert-butyl hydroperoxide- induced liver injury. Liver Int 2005; 25 (5): 1069-1073 https://doi.org/10.1111/j.1478-3231.2005.01068.x
  25. Cho WC, Chung WS, Lee SK, Leung AW, Cheng CH, Yue KK. Ginsenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats. Eur J Pharmacol 2006; 550 (1-3): 173-179 https://doi.org/10.1016/j.ejphar.2006.08.056
  26. Sung JH, So NW, Jeon BH, Chang CC. Effect of white and red Panax ginseng extract on serum lipids level in high-fat-diet fed rats. J Ginseng Res 2004; 28 (1): 33-38 https://doi.org/10.5142/JGR.2004.28.1.033
  27. W Ji, BQ Gong. Hypolipidemic effects and mechanisms of Panax notoginseng on lipid profile in hyperlipidemic rats. J Ethnopharmacol 2007; 113 (2): 318-324 https://doi.org/10.1016/j.jep.2007.06.022
  28. Fridovich I. Biological effects of the superoxide radical. Arch Biochem Biophys 1986; 247 (1): 1-11 https://doi.org/10.1016/0003-9861(86)90526-6
  29. Schraufstatter I, Hyslop PA, Jackson JH, Cochrane CG. Oxidantinduced DNA damage of target cells. J Clin Invest 1988; 82 (3): 1040-1050 https://doi.org/10.1172/JCI113660
  30. Bartoli GM, Giannattasio B, Palozza P, Cittadini A. Superoxide dismutase depletion and lipid peroxidation in rat liver microsomal membranes: correlation with liver carcinogenesis. Biochim Biophys Acta 1988; 966 (2): 214-221 https://doi.org/10.1016/0304-4165(88)90114-6
  31. Jeon BH, Seong GS, Chun SG, Sung JH, Chang CC. Antioxidant effects of white and red ginseng on liver of high fat diet-treated mice. J Ginseng Res 2005; 29 (3): 138-144 https://doi.org/10.5142/JGR.2005.29.3.138
  32. J Searle, JF Kerr, CJ Bishop. Necrosis and apoptosis: distinct modes of cell death with fundamentally different significance. Pathol Annu 1982; 17 (2): 229-259
  33. Tanaka M, Ito H, Adachi S, Akimoto H, Nishikawa T, Kasajima T, Marumo F, Hiroe M. Hypoxia induces apoptosis with enhanced expression of Fas antigen messenger RNA in cultured neonatal rat cardiomyocytes. Circ Res 1994; 75 (3): 426-433 https://doi.org/10.1161/01.RES.75.3.426
  34. Gottlieb RA, Burleson KO, Kloner RA, Babior BM, Engler RL. Reperfusion injury induces apoptosis in rabbit cardiomyocytes. J Clin Invest 1994; 94 (4): 1621-1628 https://doi.org/10.1172/JCI117504
  35. Bardales RH, Hailey LS, Xie SS, Schaefer RF, Hsu SM. In situ apoptosis assay for the detection of early acute myocardial infarction. Am J Pathol 1996; 149 (3): 821-829
  36. Gupta RK, Schuster R, Christian WD. A comparative immunohistochemical study of splenic arterial hyalinosis in health and disease. Am J pathol 1972; 69 (1): 79-88
  37. J kajstura, W Cheng, K Reiss, AW Clark, EH Sonnenblick, S Krajewski, CJ Reed, G Olivetti, P Anversa. Apoptotic and necrotic myocyte cell deaths are independent contributing variables of infarct size in rats. Lab Invest 1996; 74 (1): 86-107
  38. Hockenbery DM, Oltvai ZN, Yin XM, Milliman CL, Korsmeyer SJ. Bcl-2 functions in an antioxidant pathway to prevent apoptosis. Cell 1993; 75: 241-251 https://doi.org/10.1016/0092-8674(93)80066-N
  39. Kane DJ, Sarafian TA, Anton R, Hahn H, Gralla EB, Valentine JS, Ord T, Bredesen DE. Bcl-2 inhibition of neural death: decreased generation of reactive oxygen species. Science 1993; 262 (5137): 1274 - 1277 https://doi.org/10.1126/science.8235659
  40. Graeber TG, Osmanian C, Jacks T, Housman DE, Koch CJ, Lowe SW, Giaccia AJ. Hypoxia-mediated selection of cells with diminished apoptotic potential in solid tumours. Nature 1996; 379 (6560): 88-91 https://doi.org/10.1038/379088a0
  41. Sandstrom PA, Mannie MD, Buttke TM. Inhibition of activationinduced death in T cell hybridomas by thiol antioxidants: oxidative stress as a mediator of apoptosis. J Leukocyte Biol 1994; 55 (2): 221-226 https://doi.org/10.1002/jlb.55.2.221
  42. Cabral de Oliveira AC, Perez AC, Merino G, Prieto JG, Alvarez AI. Protective effects of panax ginseng on muscle injury and inflammation after eccentrie exercise. Comp Biochem Physiol C Toxicol Pharmacol 2001; 130 (3): 369-377 https://doi.org/10.1016/S1532-0456(01)00262-9
  43. Zhou W, Chai H, Lin PH, Lumsden AB, Yao Q, Chen CJ. Molecular mechanisms and clinical applications of ginseng root for cardiovascular disease. Med Sci Monit 2004; 10 (8): RA187-192
  44. SC Joshi. Antiatherogenic and antioxidant status of Panax ginseng in cholesterol ged rabbits. Proceedings of the 9th Int Ginseng Symposium. 2006 Sep 25-28, Keumsan, Republic of Koerea
  45. Lu JP, Ma ZC, Yang J, Huang J, Wang SR, Wang SQ. Ginsenoside Rg1-induced alterations in gene expression in TNF-$\alpha$ stimulated endothelial cells. Chinas Med 2004; 117 (6): 871-876