Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Cryopreservation of zygotic embryos of wild yams(Dioscorea spp.) in Korea

  • Shin, Jong-Hee (Institute for Bioresources, Gyeongbuk Provincial Agricultural Technology Administration) ;
  • Kang, Dong-Kyoon (Institute for Bioresources, Gyeongbuk Provincial Agricultural Technology Administration) ;
  • Lim, Jae-Ha (Institute for Bioresources, Gyeongbuk Provincial Agricultural Technology Administration) ;
  • Sohn, Jae-Keun (Department of Agronomy, Kyungpook National University)
  • Published : 2008.09.30
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Abstract

A simplified technique that cryoprotects zygotic embryos by desiccation was developed for germplasm conservation of wild yam species(Dioscorea spp.) in Korea. Comparative studies with three other cryogenic techniques were conducted. The maximum survival of zygotic embryos were achieved at a frequency of 96.6% when embryos were cryopreserved by the desiccation method. For the successful cryopreservation of yam zygotic embryos, those that were excised from immature/mature seeds were dried in the air stream of a laminar flow cabinet for 30 min at room temperature and then directly immersed in liquid nitrogen.

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References

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