Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Lymphatic Delivery of $^{99m}Tc$-labeled Dextran Acetate Particles Including Cyclosporine A

  • Kim, Jin (Department of Advanced Chemicals Graduate School, Chonnam National University) ;
  • Chung, Kyong-Hwan (Center for Functional Nano Fine Chemicals, Chonnam National University) ;
  • Lee, Chang-Moon (Department of Nuclear Medicine and Research Institute of Clinical Medicine, Chonbuk National University School of Medicine) ;
  • Seo, Young-Soon (Department of Nuclear Medicine, Chonnam National University Medical School) ;
  • Song, Ho-Chun (Department of Nuclear Medicine, Chonnam National University Medical School) ;
  • Lee, Ki-Young (Center for Functional Nano Fine Chemicals, Chonnam National University)
  • Published : 2008.09.30
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Abstract

Biodistribution and lymphoscintigraphy of cyclosporine A (CyA) and technetium-99m ($^{99m}Tc$) were studied using ${^99m}Tc$-labeled dextran acetate (DxA) including CyA. DxA particles were prepared from dextran with acetic anhydride, and CyA was loaded into them. Lymphatic delivery of ${^99m}Tc$-labeled DxA particles containing CyA was evaluated after subcutaneous injection into the foot pad of rats and compared with those of ${^99m}Tc$-labeled human serum albumin (HSA). The labeling efficiency of CyA-loaded ${^99m}Tc$-DxA particles was about 95% at 30 min. The labeling efficiency maintained stably above 80% for 12 h. The percent injected dose (%ID) of CyA-loaded ${^99m}Tc$-DxA was similar to that of ${^99m}Tc$-HSA at the inguinal lymph node after 40 min. The CyA-loaded ${^99m}Tc$-DxA could be as well distributed as ${^99m}Tc$-HSA through the lymph node. The DxA particles could steadily distribute the CyA as well as the ${^99m}Tc$ radiolabeling through the lymph node.

Keywords

References

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