Journal of Microbiology and Biotechnology

  • 제18권8호
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  • Pages.1470-1474
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  • 2008
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Nitrospira Community Composition in Nitrifying Reactors Operated with Two Different Dissolved Oxygen Levels

  • Park, Hee-Deung (R&D Institute, Kolon Engineering and Construction) ;
  • Noguera, Daniel R. (Department of Civil and Environmental Engineering, University of Wisconsin)
  • 발행 : 2008.08.31
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초록

Nitrospira is a dominant member of nitrite-oxidizing bacteria (NOB) in nitrifying bioreactors as well as in natural habitats. In this study, Nitrospira NOB were investigated in the two nitrifying reactors operated with high and low dissolved oxygen (DO) concentrations for a period of 300 days. Phylogenetic and terminal restriction fragment length polymorphism analyses based on 168 rRNA gene sequences revealed that the Nitrospira community compositions of the two reactors during the early period related to group 1 and half of the Nitrospira community composition shifted to group 2 in the high-DO reactor after day 179, although there was no significant change in the low-DO reactor. These results suggested that DO was an important factor affecting Nitrospira community compositions in the nitrifying reactors.

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참고문헌

  1. Brock, T. D. and M. D. Madigan. 1988. Biology of Microorganisms. Prentice Hall, Englewood Cliffs
  2. Burrell, P. C., J. Keller, and L. L. Blackall. 1998. Microbiology of a nitrite-oxidizing bioreactor. Appl. Environ. Microbiol. 64: 1878-1883
  3. Daims, H., J. L. Nielsen, P. H. Nielsen, K. H. Schleifer, and M. Wagner. 2001. In situ characterization of Nitrospira-like nitrite-oxidizing bacteria active in wastewater treatment plant. Appl. Environ. Microbiol. 67: 5273-5284 https://doi.org/10.1128/AEM.67.11.5273-5284.2001
  4. Hao, O. J. and J. M. Chen. 1994. Factors affecting nitrite buildup in submerged filter system. J. Environ. Eng. ASCE 120:1298-1307 https://doi.org/10.1061/(ASCE)0733-9372(1994)120:5(1298)
  5. Juretschko, S., G. Timmermann, M. Schmid, K.-H. Schleifer, A. Pommerening-Roser, H.-P. Koops, and M. Wagner. 1998. Combined molecular and conventional analyses of nitrifying bacterium diversity in activated sludge: Nitrosococcus mobilis and Nitrospira-like bacteria as dominant populations. Appl. Environ. Microbiol. 64: 3042-3051
  6. Maixner, F., D. R. Noguera, B. Anneser, K. Stoecker, G. Wegl, M. Wagner, and H. Daims. 2006. Nitrite concentration influences the population structure of Nitrospira-like bacteria. Environ. Microbiol. 8: 1487-1495 https://doi.org/10.1111/j.1462-2920.2006.01033.x
  7. Park, H.-D. and D. R. Noguera. 2004. Evaluating the effect of dissolved oxygen on ammonia-oxidizing bacterial communities in activated sludge. Water Res. 38: 3275-3286 https://doi.org/10.1016/j.watres.2004.04.047
  8. Park, H.-D., L.-M. Whang, S. R. Reusser, and D. R. Noguera. 2006. Taking advantage of aerated-anoxic operation in a fullscale University of Cape Town process. Water Environ. Res. 78:637-642 https://doi.org/10.2175/106143006X99786
  9. Prosser, J. I. 1989. Autotrophic nitrification in bacteria. Adv. Microb. Physiol. 30: 125-181
  10. Regan, J. M., G. W. Harrington, and D. R. Noguera. 2002. Ammonia- and nitrite-oxidizing bacterial communities in a pilot-scale chloraminated drinking water distribution system. Appl. Environ. Microbiol. 68: 73-81 https://doi.org/10.1128/AEM.68.1.73-81.2002
  11. Schramm, A., D. De Beer, M. Wagner, and R. Amann. 1998. Identification and activities in situ of Nitrosospira and Nitrospira spp. as dominant populations in a nitrifying fluidized bed reactor. Appl. Environ. Microbiol. 64: 3480-3485
  12. Simm, R. A., D. S. Mavinic, and W. D. Ramey. 2006. A targeted study on possible free ammonia inhibition of Nitrospira. J. Environ. Eng. Sci. 5: 365-376 https://doi.org/10.1139/S05-044
  13. Sliekers, A. O., S. C. M. Haaijer, M. H. Stafsnes, J. G. Kuenen, and M. S. M. Jetten. 2005. Competition and coexistence of aerobic ammonium- and nitrite-oxidizing bacteria at low oxygen concentrations. Appl. Microbiol. Biotechnol. 68: 808-817 https://doi.org/10.1007/s00253-005-1974-6
  14. Spieck, E., C. Hartwig, I. McCormack, F. Maixner, M. Wagner, A. Lipski, and H. Daims. 2006. Selective enrichment and molecular characterization of a previously uncultured Nitrospira-like bacterium from activated sludge. Environ. Microbiol. 8: 405-415 https://doi.org/10.1111/j.1462-2920.2005.00905.x
  15. Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. G. Higgins. 1997. The CLUSTAL_X Windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25: 4876-4882 https://doi.org/10.1093/nar/25.24.4876
  16. US-EPA. 1993. Manual: Nitrogen Control. US-EPA, Cincinnati, OH
  17. Watson, S. W., E. Bock, H. Harms, H.-P. Koops, and A. B.Hooper. 1989. Nitrifying bacteria, pp. 1808-1834. In J. T. Staley, M. P. Bryant, N. Pfenning, and J. G. Holt (eds.), Bergey's Manual of Systematic Bacteriology, vol. III. Williams and Wilkins, Baltimore, MD.