Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Integral Antioxidative Capacity of Extracts by Pressurized Organic Solvent from Natural Plants in Jeju

제주 자생식물 고압용매 추출물의 통합적 항산화 능력

  • Kim, Mi-Bo (Dept. of Food Bioengineering, Cheju National University) ;
  • Hyun, Sun-Hee (Dept. of Food Bioengineering, Cheju National University) ;
  • Park, Jae-Sung (Dept. of Food Bioengineering, Cheju National University) ;
  • Kang, Mi-Ae (Dept. of Food Bioengineering, Cheju National University) ;
  • Ko, Young-Hwan (Dept. of Food Bioengineering, Cheju National University) ;
  • Lim, Sang-Bin (Dept. of Food Bioengineering, Cheju National University)
  • 김미보 (제주대학교 식품생명공학과) ;
  • 현선희 (제주대학교 식품생명공학과) ;
  • 박재성 (제주대학교 식품생명공학과) ;
  • 강미애 (제주대학교 식품생명공학과) ;
  • 고영환 (제주대학교 식품생명공학과) ;
  • 임상빈 (제주대학교 식품생명공학과)
  • Published : 2008.11.28
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Abstract

Twenty natural plants from Jeju were extracted by pressurized organic solvent (100% methanol, $40^{\circ}C$, 13.6 MPa, 10 min). Extraction yield, total phenolic content (TPC) and integral antioxidant capacity were measured, and each component was identified by GC/MS. Extraction yields were high as 21.8%, 21.5, 21.1, 20.7 and 20.1% in Rhus javanica, Euscaphis japonica, Alnus firma, Sapium japonicum and Sorbus alnifolia, respectively. The extracts containing high TPC (mg GAE/g of dry sample) were obtained from Malus sieboldii (68.3), Sapium japonicum (57.6), Pyrrosia lingua (56.6) and Euscaphis japonica (55.1). Integral antioxidant capacities of water-soluble substances were 598, 394, 293 and $270\;{\mu}mol$ ascorbic acid equivalent/g in Geranium thunbergii, Sapium japonicum, Cornus kousa and Rhus javanica, respectively. Integral antioxidant capacities of lipidsoluble substances were 611, 314, 296 and $242\;{\mu}mol$ trolox equivalent/g in Ardisia crenata, Ostrya japonica, Geranium thunbergii and Quercus acuta, respectively. Fifteen major peaks were identified by GC/MS from the extract of pressurized organic solvent from Sapium japonicum. Two polyphenols (gallic acid (retention time (RT) 19.7 min)) and quercetin (33.5 min)), ascorbic acid (RT 35.3 min), and several fatty acids (retention time 18.6, 21.0, 21.8, 21.9 and 23.6 min) were identified, and gallic acid was the major polyphenol component due to high peak area.

제주 자생식물 20종을 대상으로 고압용매 추출(추출용매 100% methanol, 추출 온도 $40^{\circ}C$, 추출 압력 13.6 MPa, 추출 시간 10분)하여 총페놀 함량과 통합적 항산화 능력을 측정하고 폴리페놀 성분을 동정하였다. 추출수율은 붉나무, 말오줌때, 사방오리나무, 사람주나무, 팥배나무가 각각 21.8, 21.5, 21.1, 20.7, 20.1%로 가장 높았다. 총페놀 함량은 아그배가 68.3 mg GAE/g로 가장 높았고, 다음으로 사람주나무, 석위, 말오줌때가 각각 57.6, 56.6, 55.1 mg GAE/g을 나타내었다. 수용성 항산화 능력은 이질풀, 사람주나무, 산딸나무, 붉나무가 각각 598, 394, 293, $270\;{\mu}mol$ ascorbic acid equivalent/g로 높았고, 지용성 항산화 능력은 백량금, 새우나무, 이질풀, 붉가시나무가 611, 314, 296, $242\;{\mu}mol$ trolox equivalent/g로 높았다. GC/MS에 의한 폴리페놀성분을 동정한 결과 15개의 주요 피크를 얻었으며, 그 중 2종의 폴리페놀류(gallic acid(체류시간 19.7분)와 quercetin(체류시간 33.5분)), ascorbic acid(체류시간 35.3분) 그리고 다수의 지방산류(체류시간 18.6, 21.0, 21.8, 21.9, 23.6분)를 확인할 수 있었는데, 이 중 gallic acid는 다른 성분보다 peak area가 높은 것으로 나타나 사람주나무의 가장 중요한 폴리페놀 성분으로 추정되었다.

Keywords

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