Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Nutritional Component and Anticancer Properties of Various Extracts from Haesongi Mushroom (Hypsizigus marmoreus)

해송이버섯(Hypsizigus marmoreus)의 영양성분과 추출용매에 따른 암세포 생장억제 효과

  • Published : 2008.11.28
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Abstract

This study was aimed to analyze the nutritional components and anticancer properties of Haesongi mushroom (Hypsizigus marmoreus), which has been recently available in Korea, to estimate its nutritional and functional values. Fruit body of Haesongi mushroom was investigated for its proximate components and mineral contents. Its water and ethanol extracts were compared for nutritional components such as $\beta$-glucan, protein, and total sugar. Anticancer effects of both extracts were measured against human cancer cell lines in vitro. This mushroom contained high protein (22.63%), total dietary fiber (30.80%), and K (3383.3 mg/100 g). The water extract contained more nutritional components such as $\beta$-glucan (9.32 mg/g), protein (17.71%), and total sugar (39.93%), compared with the ethanol extract. Moreover the extraction yield of the water extract was higher than the ethanol extract. The growth inhibitory effects of the water extract (5 mg/mL) on AGS, HepG2, and SW480 human cancer cells were 90.61, 75.43, and 58.49%, respectively. However, the ethanol extract showed 81.79, 49.90, and 25.71% growth inhibition, respectively. In this study, it is demonstrated that water is a more efficient solvent than ethanol for extracting nutritional and functional components from Haesongi mushroom.

본 연구에서는 해송이버섯의 영양 가치와 활용도를 높이기 위해 영양성분을 분석하였으며 효과적으로 영양성분을 추출하기 위한 추출용매를 설정하고 그 추출물을 이용하여 in vitro 상에서 항암활성을 검토하였다. 그 결과 해송이 버섯은 30.80%의 식이섬유를 포함하는 것으로 나타났다. 해송이버섯의 무기질 중 칼륨의 함량이 3383.3 mg/100 g으로 다른 무기원소에 비해 월등히 높게 나타났다. 해송이버섯을 물을 이용하여 추출하였을 경우 $\beta$-glucan 9.32 mg/g, 단백질 17.71% 및 총당 39.93%이었으며 에탄올을 이용하여 추출할 때보다 더 많은 영양성분들이 추출되는 것으로 나타났고 추출물의 수율 또한 53.34%로 높았다. 각각의 해송이버섯추출물에 의한 암세포 생장억제효과를 사람의 위암세포인 AGS와 간암세포 HepG2, 대장암세포 SW480으로 검토한 결과, 각 추출물의 농도에 따라 암세포 생장억제율이 증가함을 보여주었고, 물추출물이 에탄올추출물보다 상대적으로 높은 억제율을 보였다. AGS에 대해서는 물추출물과 에탄올 추출물 모두 1 mg/mL의 농도에서 모두 50%이상의 생장억제효과를 나타내었다. 물추출물의 경우 HepG2에 대해서는 3 mg/mL 이상의 농도에서, SW480에 대해서는 5 mg/mL의 농도에서 50%이상의 억제효과를 나타내었다. 반면 에탄올 추출물은 HepG2와 SW480에 대한 생장억제효과는 5 mg/mL의 농도에서도 50%미만의 억제효과를 나타내었다.

Keywords

References

  1. Kim HS, Ha HC, Kim TS. 2003. Research and prospects in new functional mushrooms-Tremella fuciformis, Grifora frondosa and Hypsizigus marmoreus. Food Sci Ind 36(4): 42-46
  2. Yang HC, Song CH, Kweon MH. 1996. Mycelial new material, food functional technology. Hanlim, Seoul. p 187-189
  3. Misuno T. 1990. Antitumor activity and some properties of water soluble polysaccharides from fruiting body of Agaricus blazei Murill. Agric Biol Chem 54: 2889-2896 https://doi.org/10.1271/bbb1961.54.2889
  4. Hirokazu K, Ryuichi I, Teturo K, Takashi M. 1989. Fractionation and antitumor activity of the water in soluble residue of Agaricus blazei fruiting bodies. Carbohydr Res 186: 267-273 https://doi.org/10.1016/0008-6215(89)84040-6
  5. Nakajima A, Ishida T, Koga M, Takeuchi M. 2002. Effect of hot water extract from Agaricus blazei Murill on antibody-producing cells in mice. Int Immunopharmacol 2: 1205-1211 https://doi.org/10.1016/S1567-5769(02)00056-5
  6. Hui LC, Guei RC, Chin CC, Jeng LM. 2001. Non-volatile taste components of Agaricus blazei, Antrodia camphorata and Cordycps millitaris mycelia. Food Chem 74: 203-207 https://doi.org/10.1016/S0308-8146(01)00127-3
  7. Mizuno M, Morimoto M, Minato K, Tsuchida H. 1998. Polysaccharides from Agaricus blazei stimulate lymphocyte T-cell subsets in mice. Biosci Biotechnol Biochem 62: 434-437 https://doi.org/10.1271/bbb.62.434
  8. Lee YL, Yen MT, Mau JL. 2007. Antioxidant properties of various extracts from Hypsizigus marmoreus. Food Chem 104: 1-9 https://doi.org/10.1016/j.foodchem.2006.10.063
  9. Stamets P. 1993. Growing gourmet and medicinal mushrooms. Ten Speed Press, Berkeley, CA
  10. Kim HK, Choi YJ, Jeong SW, Kim KH. 2002. Functional activities of microwave-assisted extracts from Lyophyllum ulmarium. Korean J Food Presev 9: 385-390
  11. Xu ML, Choi JY, Jeong BS, Li G, Lee KR, Lee CS, Woo MH, Lee ES, Jahng Y, Chang HW, Lee SH, Son JK. 2007. Cytotoxic constituents isolated from the fruit bodies of Hypsizigus marmoreus. Arch Pharm Res 30: 28-33 https://doi.org/10.1007/BF02977775
  12. Matsuzawa T, Sano M, Tomita I, Saitoh H, Ohkawa M, Ikekawa T. 1998. Studies on antioxidants of Hypsizigus marmoreus. II. Effects of Hypsizigus marmoreus for antioxidants activities of tumor-bearing mice. Yakugaku Zasshi 118: 467-481
  13. Ikekawa T, Saitoh H, Feng W, Zhang H, Li L, Matsuzawa T. 1992. Antitumor activity of Hypsizigus marmoreus. I Antitumor activity of extracts and polysaccharides. Chem Pharm Bull 40: 1954-1957 https://doi.org/10.1248/cpb.40.1954
  14. Lam SK, Ng TB. 2001. Hypsin, a novel thermostable ribosome-inactivating protein with antifungal and antiproliferative activities from fruiting bodies of the edible mushroom Hypsizigus marmoreus. Biochem Biophys Res Comm 285: 1071-1075 https://doi.org/10.1006/bbrc.2001.5279
  15. Xu XM, Jun JY, Jeong IH. 2007. A study on the antioxidant activity of Hae-songi mushroom (Hypsizigus marmoreus) hot water extracts. J Korean Soc Food Sci Nutr 36: 1351-1357 https://doi.org/10.3746/jkfn.2007.36.11.1351
  16. AOAC. 1995. Official Method of Analysis of AOAC Intl. 16th ed. Association of Official Analytical Communities, Arlington, VA, USA
  17. Korea Food and Drug Administration. 2005. Food Standards Codex. Korean Foods Industry Association, Seoul, Korea
  18. Saha SK, Brewer CF. 1994. Determination of the concentrations of oligosaccharides, complex type carbohydrates, and glycoproteins using the phenol-sulfuric acid method. Carbohydr Res 254: 157-167 https://doi.org/10.1016/0008-6215(94)84249-3
  19. Lowry OH, Rosebrough NJ, Farr Al, Randall RJ. 1954. Protein measurement with the Folin phenol reagents. J Biol Chem 193: 265-275
  20. Green LM, Reade JL, Ware CF. 1984. Rapid colorimetric assay for cell viability: application to the quantitation of cytotoxic and growth inhibitory lymphokines. J Immunol Methods 70: 257-268 https://doi.org/10.1016/0022-1759(84)90190-X
  21. Hong KH, Kim BY, Kim HK. 2004. Analysis of nutritional components in Pleutorus ferulea. Korea J Food Sci Tehchol 36: 563-567
  22. Kang TS, Kang MS, Sung JM, Kang AS, Shon HR, Lee SY. 2001. Effect of Pleurotus eryngii on the blood glucose and cholesterol in diabetic rats. Korea J Mycol 29: 86-90
  23. Hwang JB, Yang MO, Shin HK. 1997. Survey for approximate composition and mineral content of medicinal herbs. Korean J Food Sci Technol 29: 671-679
  24. Krishna GG, Miller E, Kapoor S. 1989. Increased blood pressure during potassium depletion in normotensive men. New Eng J Med 320: 1177-1182 https://doi.org/10.1056/NEJM198905043201804
  25. Lee YS, Kim JB, Shin SR, Kim NW. 2006. Analysis of nutritional components of Lepista nuda. Korean J Food Preserv 13: 375-381
  26. Bobek P, Ozdin L, Kuniak L. 1993. Influence of water and ethanol of the oyster mushroom (Pleurotus ostreatus) on serum and liver lipids of the Syrian hamsters. Die Nahrung 37: 571-575 https://doi.org/10.1002/food.19930370608
  27. Ballance GM, Manners DJ. 1978. Structural analysis and enzymic solubilization of barley endosperm cell walls. Carbohydr Res 61: 107-113 https://doi.org/10.1016/S0008-6215(00)84471-7
  28. Hong JH, Youn KS, Choi YH. 2004. Characteristics of crude protein-bound polysaccharide from Agaricus blasei Murill by extraction and precipitation conditions and its antitumor effect. Korean J Food Sci Technol 36: 586-593
  29. Song JH, Lee HS, Hwang JK, Han JW, Han JG, Keum DH, Park KM. 2003. Physiological activity of Sarcodon aspratus extracts. Korean J Food Sci Ani Resour 23: 172-179
  30. Song JH, Lee HS, Hwang JK, Chung TY, Hong SR, Park KM. 2003. Physiological activities of Phellinus ribis extracts. Korean J Food Sci Technol 35: 690-695
  31. Yang KH, Yang JH, Ryu BH. 1997. Antitumor effects of extracts obtained from Daedalea dickinsii. Kor J Appl Microbiol Biotechnol 25: 178-182
  32. Yoon SH, Lim JH, Kim YS, Kim CH, Jo JH, Hwang YS. 1998. Pharmacological effects of proteoglycans extracted from fruiting bodies of Formitella fracinea. Korea J Mycol 26: 511-518
  33. Kim HJ, Han J, Yang EJ, Lee KR, Lee IS. 2000. Chemoprevention effect of Polyozellus multiplex, a wild and edible mushroom. J Korean Soc Food Sci Nutr 29: 161-167

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