Inhibition of osteoclast formation by putative human cementoblasts

  • Kim, Mi-Ri (Department of Dentistry, Asan Medical Center, University of Ulsan) ;
  • Yang, Won-Kyung (Department of Dentistry, Asan Medical Center, University of Ulsan) ;
  • Grzesik, Wojciech (Department of Anatomy and Cell Biology, School of Dental Medicine, University of Pennsylvania) ;
  • Ko, Hyun-Jung (Department of Dentistry, Asan Medical Center, University of Ulsan)
  • Published : 2008.09.30

Abstract

Cementum is the mineralized tissue of the tooth. It is similar to bone in several aspects but it differs from bone. Human bone marrow stromal cells (BMSC) and human cementum derived cells (HCDC) (10,000 $cells/cm^2$) were plated in 6 well plates as feeder cells. The next day, mouse bone marrow cells (1.5 million $cells/cm^2$) were added. One group of these plates were incubated in serum-free conditioned medium (SFCM) generated from BMSC or HCDC supplemented with 2% FBS, parathyroid hormone (PTH), 1, 25 dihydroxyvitamin $D_3$ (Vit. $D_3$) and dexamethasone, or plain medium with the same supplements. Another group of plates were cocultured with BMSC or HCDC in plain medium supplemented with 2% FBS, PTH, Vit. $D_3$ and dexamethasone. Plates grown without SFCM or coculture were used as controls. After 10 days, the cells were stained for tartrate-resistant acid phosphatase (TRAP). BMSC were found to support osteoclast formation under normal conditions. This was inhibited however by both SFCM generated from HCDC and also by coculture with HCDC. In addition, HCDC themselves did not support osteoclast formation under any conditions. Our results thus indicate that HCDC do not support osteoclast formation in vitro and that soluble factor (s) from HCDC may inhibit this process. In addition, we show that this inhibition also involves an active mechanism that is independent of osteoprotegerin, a feature that may distinguish cementoblasts from other cells present in periodontium.

Keywords

References

  1. Bell N. RANK ligand and modulation of skeletal remodeling. J Clin Invest. 2003;111:1120-1122 https://doi.org/10.1172/JCI18358
  2. Bianco P, Kuznetsov SA, Riminucci M, Fisher LW, Spiegel AM, Robey P. A miniature of human fibrous dysplasia of bone reproduced in immunodeficient mice by transplantation of mosaics of normal and Gs alpha-mutated marrow stromal fibroblasts. J Clin Invest. 1998;101:1737- 1744 https://doi.org/10.1172/JCI2361
  3. Bosshardt DD, Schroeder HE. Cementogenesis reviewed : A comparison between human premolars and rodent molars. Anat Rec. 1996;245:267-292 https://doi.org/10.1002/(SICI)1097-0185(199606)245:2<267::AID-AR12>3.0.CO;2-N
  4. Cha Ji-Hun, Ryoo HM, Woo KM, Kim GS, Baek JH. Dlx3 Plays a Role as a Positive Regulator of Osteoclast Differentiation. Int J Oral Biol. 2007;32(3):85-91
  5. Freeman E. Periodontium. In:Ten Cate AR(ed.) Oral Histology, 4th Ed., pp.276-312, Mosby-Year Book, Inc., St. Louis, MO, U.S.A., 1994
  6. Grzesik WJ, Kuznetsov SA, Uzawa K, Mankani M, Gehron Robey P, Yamauchi M. Normal human cementum-derived cells: isolation, clonal expansion and in vitro and in vivo characterization. J Bone Miner Res. 1998;13:1547-1554 https://doi.org/10.1359/jbmr.1998.13.10.1547
  7. Grzesik WJ, Cheng H, Oh JS, Kuznetsov SA, Mankani MH, Uzawa K, Robey PG, Yamauchi M. Cementun-forming cells are phenotypically distinct from bone-forming cells. J Bone Miner Res. 2000;15:52-59 https://doi.org/10.1359/jbmr.2000.15.1.52
  8. Hasegawa T, Yoshimura Y, Kikuiri T, Yawaka Y, Takeyama S, Matsumoto A, Oguchi H, Shirakawa T. Expression of receptor activator of NF-kappa B ligand and osteoprotegerin in culture of human periodontal ligament cells. J Periodontal Res. 2002;37(6):405-11 https://doi.org/10.1034/j.1600-0765.2002.01603.x
  9. Hatakeyama J, Sreenath T, Hatakeyama Y, Thyagarajan T, Shum L, Gibson CW. The receptor activator of nuclear factor-kappa B ligand-mediated osteoclastogenic pathway is elevated in amelogenin-null mice. J Biol Chem. 2003;12; 278(37): 35743-8
  10. Jones SJ, Arora M, Boyde A. The rate of osteoclastic destruction of calcified tissues is inversely proportional to mineral density. Calcif Tissue Int. 1995;56(6):554-8 https://doi.org/10.1007/BF00298589
  11. Katz RW, Hollinger JO, Reddi AH. The functional equivalence of demineralized bone and tooth matrices in ectopic bone induction. J Biomed Mater Res. 1993;27(2):239-45 https://doi.org/10.1002/jbm.820270214
  12. Krebsbach PH, Kuznetsov SA, Satomura K, Emmons RVB, Rowe DW, Robey PG. Bone formation in vivo comparison of osteogenesis by transplanted mouse and human marrow stromal fibroblasts. Transplantation. 1997;63:1059-1069 https://doi.org/10.1097/00007890-199704270-00003
  13. Kuznetsov SA, Krebsbach PH, Satomura K, Kerr J, Rimminucci M, Benayahu D, Robey PG. Single colony derived strains of human marrow fibroblasts form bone after transplantation in vitro. J Bone Miner Res. 1997;12:1335- 1347 https://doi.org/10.1359/jbmr.1997.12.9.1335
  14. Oshiro T, Shiotani A, Shibasaki Y, Sasaki T. Osteoclast induction in periodontal tissue during experimental movement of incisors in osteoprotegerin-deficient mice. Anat Rec. 2002;1;266(4):218-25
  15. Roux S and Orcel P. Bone loss factors that regulate osteoclast differentiation. : an update. Arthritis Res. 2000;2;451-456 https://doi.org/10.1186/ar127
  16. Suda T, Takahashi N, Udagawa N, Jimi E, Gillespie MT, Martin TJ. Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr Rev. 1999;20(3): 345-57 https://doi.org/10.1210/er.20.3.345
  17. Wise GE, Yao S, Zhang Q, Ren Y. Inhibition of osteoclastogenesis by the secretion of osteoprotegerin in vitro by rat dental follicle cells and its implications for tooth eruption. Arch Oral Biol. 2002;47(3):247-54 https://doi.org/10.1016/S0003-9969(01)00109-1