International Journal of Industrial Entomology and Biomaterials

  • 제16권2호
  • /
  • Pages.87-92
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  • 2008
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  • 3022-7542(pISSN)
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  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
권호 표지

High-level Expression, Polyclonal Antibody Preparation and Bioinformatics Analysis of Bombyx mori Nucleopolyhedrovirus orf47 Encodes Protein

  • Wu, Chao (Institute of Life Sciences, Jiangsu University) ;
  • Guo, Zhongjian (Institute of Life Sciences, Jiangsu University) ;
  • Chen, Keping (Institute of Life Sciences, Jiangsu University) ;
  • Shen, Hongxing (Institute of Life Sciences, Jiangsu University)
  • 발행 : 2008.06.30
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초록

Bombyx mori nucleopolyhedrovirus (BmNPV) orf47 gene was characterized for the first time. The coding sequence of Bm47 was amplified and subcloned into the prokaryotic expression vector pET-30a(+) in order to produce His-tagged fusion protein in the BL21 (DE3) cells. The His-Bm47 fusion protein was expressed efficiently after induction with IPTG. The purified fusion protein was used to immunize New Zealand white rabbits to prepare polyclonal antibody. As the genome of BmNPV is available in GenBank and the EST database of BmNPV is expanding, identification of novel genes of BmNPV was conceivable by data-mining techniques and bioinformatics tools. Structural bioinformatics approach to analyze the properties of Bm47 encodes protein.

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