Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
권호 표지

REMINERALIZATION EFFECT OF COMMERCIALLY AVAILABLE ANTICARIOGENIC PRODUCTS ON ARTIFICIAL CARIES LESION USING QLF

QLF를 이용한 항우식 제품의 인공우식 재광화 효과에 대한 연구

  • Song, Ju-Hyun (Department of Pediatric Dentistry, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Kim, Young-Jae (Department of Pediatric Dentistry, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Kim, Jung-Wook (Department of Pediatric Dentistry, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Jang, Ki-Taeg (Department of Pediatric Dentistry, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Kim, Chong-Chul (Department of Pediatric Dentistry, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Hahn, Se-Hyun (Department of Pediatric Dentistry, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Lee, Sang-Hoon (Department of Pediatric Dentistry, School of Dentistry and Dental Research Institute, Seoul National University)
  • 송주현 (서울대학교 치과대학 소아치과학교실) ;
  • 김영재 (서울대학교 치과대학 소아치과학교실) ;
  • 김정욱 (서울대학교 치과대학 소아치과학교실) ;
  • 장기택 (서울대학교 치과대학 소아치과학교실) ;
  • 김종철 (서울대학교 치과대학 소아치과학교실) ;
  • 한세현 (서울대학교 치과대학 소아치과학교실) ;
  • 이상훈 (서울대학교 치과대학 소아치과학교실)
  • Published : 2008.05.30
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Abstract

The purpose of this in vitro study was to compare the remineralization effect of commercially available anticariogenic products, exactly low level fluoride mouthrinse(500 ppm NaF), tooth cream with Casein phosphopeptide-amorphous calcium phosphate(CPP-ACP) and fluoride mouthrinse plus tooth cream on artificial caries lesion. Artificial caries lesion was induced at the buccal surface of permanent third molar and the specimens were then divided(16 specimens/group) into four group. Twice a day during 28 days specimens of each group were treated with a commercially anticariogenic product as follows and applied to the pH cycling system. Group 1: control group (No treatment) Group 2: Tooth $Mousse^{(R)}$ (GC Co. Japan) Group 3: $chikachika^{(R)}$ (Samil Co. Korea) Group 4: $chikachika^{(R)}$+Tooth Mousse$^{(R)}$ The long-term change of mineral loss(${\Delta}Q$) was evaluated by quantitative light-induced fluorescence (QLF) and the following results were obtained: 1. ${\Delta}Q$ of Group 1 was not noticed statistically significant during 28 days comparing that prior to treatment. There was a statistically significant increase in ${\Delta}Q$ of Group 2 and 3 since 14 days. So was in ${\Delta}Q$ of Group 4 since 7 days. 2. ${\Delta}Q$ was increased as follows: Group 1< Group 2, 3< Group 4. 3. Comparing with Group 1, Group 2 was a statistically significant increase since 7 days and Group 3 and 4 were since 3 days. Comparing Group 2 with 3, there was not noticed statistically significant during whole duration. Group 4 was significantly higher than Group 2 and 3 after 28 days. 4. All groups demonstrated a decrease in the rate of remineralization as time goes on.

본 연구는 항우식 제품인 저농도의 불소 양치액(500 ppm NaF)과 Casein phosphopeptide-amorphous calcium phosphate(CPP-ACP)를 포함하는 치아 연고, 그리고 이 두 제품을 같이 사용했을 경우, 인공 우식 병소의 재광화 효과를 알아보고자 하였다. 제3대구치 협면에 인공우식을 유발하였고, 시편을 16개씩 4군으로 나누어, 28일 동안 매일 2 회 다음과 같은 항우식 제품을 시편에 처리하였고, pH 순환 시스템을 적용하였다. 1군: 대조군(무처치) 2군: Tooth $Mousse^{(R)}$ (GC Co. Japan) 3군: 치카치카$^{(R)}$ (삼일제약. 한국) 4군: 치카치카$^{(R)}$+Tooth Mousse$^{(R)}$ 무기질 소실량(${\Delta}Q$)의 장기적인 변화를 Quantitative light-induced fluorescence(QLF)를 이용하여 관찰하여 다음과 같은 결과를 얻었다. 1. ${\Delta}Q$는 1군에서는 28일동안 유의성 있는 차이를 보이지 않았고, 2군과 3군에서는 처치전에 비해 14일부터 유의성 있는 증가를 보였으며, 4군에서는 7일부터 유의성 있는 증가를 보였다. 2. ${\Delta}Q$는 1군 < 2군, 3군 < 4군 순이었다. 3. ${\Delta}Q$는 1군과 비교시 2군은 7일, 3군과 4군은 3일부터 유의성 있는 증가를 보였고, 2군은 3군과 전 기간 동안 유의차가 없었다. 4군은 2군과 3군에 비해 28일에 유의성 있는 증가를 보였다. 4. 모든 군에서 재광화 속도는 점차 감소하였다.

Keywords

References

  1. Meller C, Heyduck C, Tranaeus S, et al. : A new in vivo method for measuring caries activity using Quantitative light-induced fluorescence. Caries Res, 40:90-96, 2006. https://doi.org/10.1159/000091053
  2. Ismail AI, Brodeur JM, Gagnon P, et al. : Prevalence of non-cavitated and cavitated carious lesions in a random sample of 7-9-year school children in Montreal Quebec. Community Dent Oral Epidmiol, 20:250-255, 1992. https://doi.org/10.1111/j.1600-0528.1992.tb01693.x
  3. Lijima Y, Takagi O, Ruben J, et al. : In vitro remineralization of in vivo and in vitro formed enamel lesions. Caries Res, 33:206-213, 1999. https://doi.org/10.1159/000016518
  4. Hicks J, Garcia-Godoy F, Flaitz C : Biological factors in dental caries enamel structure and the caries process in the dynamic process of demineralization and remineralization (part 2). J Clin Pediatr Dent, 28:119-24, 2004.
  5. Mount GJ : Defining, classifying, and placing incipient caries lesions in perspective. Dent Clin North Am, 49:701-23, 2005. https://doi.org/10.1016/j.cden.2005.05.012
  6. Kashket S : Historical review of remineralization research. J Clin Dent, 10: 56-64, 1999.
  7. Zero DT : Application of clinical models in remineralization research. J Clin Dent, 10:74-85, 1999.
  8. Pretty IA, Pender N, Edgar WM, et al. : The in vitro detection of early enamel de- and re-mineralization adjacent to bonded orthodontic cleats using quantitative light-induced fluorescence. Eur J Orthod, 25:217-23, 2003. https://doi.org/10.1093/ejo/25.3.217
  9. Higham SM, Pretty IA, Edgar WM, et al. : The use of in situ models and QLF for the study of coronal caries. J Dent, 33:235-41, 2005. https://doi.org/10.1016/j.jdent.2004.10.016
  10. Stookey GK : Quantitative light fluorescence: a technology for early monitoring of the caries process. Dent Clin North Am, 49:753-70, 2005. https://doi.org/10.1016/j.cden.2005.05.009
  11. Takagi S, Liao H, Chow LC : Effect of a low-fluoride- content, two-component rinse on fluoride uptake and on de-and remineralization of enamel lesions: an in vitro study. Caries Res, 35:223-228, 2001. https://doi.org/10.1159/000047460
  12. 문성권, 이재천, 김영재, 등. : Fluoride varnish와 acidulated phosphate gel이 인공우식 병소에 미치는 영향에 대한 미세전산화 단층 촬영을 이용한 연구. 대한소아치과 학회지, 31:212-22, 2004.
  13. Silverstone LM : Structure of carious enamel, including the early lesion. Oral Sci Rev, 3:100-160, 1973.
  14. 이창근, 김종수, 유승훈 : Quantitative light-induced fluorescence를 이용한 법랑질 탈회 속도에 관한 비교 연 구. 대한소아치과학회지, 31:506-515, 2004.
  15. Benson PE, Pender N, Higham SM : An in situ caries model to study demineralization during fixed orthodontics. Clin Orthod Res, 2:143-153, 1999. https://doi.org/10.1111/ocr.1999.2.3.143
  16. Ogaard B, ROlla G : The in vivo orthodontic banding model for vital teeth and the in situ orthodontic banding model for hard tissue slabs. J Dent Res, 71(special issue):832-35, 1992.
  17. Featherstone JD, ten Cate JM, Shariati M, et al. : Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. Caries Res, 17:385-391, 1983. https://doi.org/10.1159/000260692
  18. Arends J, ten Bosch JJ : Demineralization and remineralization evaluation techniques. J Dent Res, 71(special issue):924-928, 1992.
  19. 황규선, 김종수, 유승훈 : Digital imaging fiber-optic trans-illumination과 laser fluorescence를 이용한 평활 면 초기우식증의 재광화에 관한 비교 연구. 대한소아치과 학회지, 34:183-191, 2007.
  20. Yamaguchi K, Miyazaki M, Takamizawa T, et al. : Effect of CPP-ACP paste on mechanical properties of bovine enamel as determined by an ultrasonic device. J Dent, 34:230-6, 2006. https://doi.org/10.1016/j.jdent.2005.06.005
  21. Aljehani A, Tranæus S, Forsberg CM, et al. : In vitro quantification of white spot enamel lesions adjacent to fixed orthodontic appliances using quantitative light-induced fluorescence and DIAGNOdent. Acta Odontol Scand, 62:313-318, 2004.
  22. LeGeros RZ : Calcium phosphates in demineralization/ remineralization processes. J Clin Dent, 10:65- 73, 1999.
  23. Featherstone JD : Prevention and reversal of dental caries: role of low level fluoride. Community Dent Oral Epidemiol, 27:31-40, 1999. https://doi.org/10.1111/j.1600-0528.1999.tb01989.x
  24. Hicks J, Garcia-Godoy F, Flaitz C : Biological factors in dental caries: role of remineralization and fluoride in the dynamic process of demineralization and remineralization (part 3). J Clin Pediatr Dent, 28:203-14, 2004. https://doi.org/10.17796/jcpd.28.3.w0610427l746j34n
  25. Ingram GS, Morgan SN : The effect of low levels of fluoride on seeded hydoxyapatite crystal growth. J Dent Res, 64:676, 1895.
  26. Gibbs CD, Atherton SE, Huntington E, et al. : Effect of low levels of fluoride on calcium uptake by demineralized human enamel. Arch Oral Biol, 40:879-81, 1995. https://doi.org/10.1016/0003-9969(95)00041-M
  27. Zero DT, Raubertas RF, Pedersen AM, et al. : Fluoride concentrations in plaque, whole saliva and ductal saliva after applications of home-use fluoride agents. J Dent Res, 71:1768-75, 1992. https://doi.org/10.1177/00220345920710110201
  28. Arends J, Chirstoffersen J : Nature and role of loosely bound fluoride in dental caries. J Dent Res, 69(Spec Iss):601-605, 1990. https://doi.org/10.1177/00220345900690S118
  29. ten Cate JM, van Loveren C : Fluoride mechanisms. Dent Clin North Am, 43:713-42, 1999.
  30. Reynolds EC, Black CL, Cai F, et al. : Advances in enamel remineralization: casein phosphopeptideamorphous calcium phosphate. J Clin Dent, 10:86- 88, 1999.
  31. Reynolds EC : Anticariogenic complexs of amorphous calcium phosphate stabilized by casein phosphopeptides: a review. Spec Care Dentist, 18:8-16, 1998. https://doi.org/10.1111/j.1754-4505.1998.tb01353.x
  32. Tung MS, Eichmiller FC : Dental applications of amorphous calcium phosphates. J Clin Dent, 10:1- 6, 1999.
  33. Shen P, Cai F, Nowicki A, Vincent J, et al. : Remineralization of enamel subsurface lesions by sugar-free chewing gum containing phosphopeptideamorphous calcium phosphate. J Dent Res, 80:2066-2070, 2001 https://doi.org/10.1177/00220345010800120801
  34. Takagi S, Liao H, Chow LC : Effect of tooth-bound fluoride on enamel demineralization/ remineralization in vitro. Caries Res, 34:281-8, 2000. https://doi.org/10.1159/000016603
  35. Silverstone LM : Effect of oral fluid and synthetic calcifying fluids in vitro on remineralization of enamel lesion. Clin Prev Dent, 4:13-22, 1981.
  36. Hicks MJ, Flaitz CM, Silverstone LM : Initiation and progression of caries-like lesions of enamel: effect of periodic treatment with synthetic saliva and sodium fluoride. Caries Res, 19:481-9, 1985. https://doi.org/10.1159/000260886
  37. Al-Khateeb S, Oliveby A, de Josselin JE, et al. : Laser fluorescence quantification of remineralization in situ of incipient enamel lesions: influence of fluoride supplements. Caries Res, 31:132-40, 1997. https://doi.org/10.1159/000262388
  38. Ogaard B, ten Bosch JJ : Regression of white spot enamel lesions: a new optical method for quantitative longitudinal evaluation in vivo. Am J Orthod Dentofacial Orthop, 106:238-42, 1994. https://doi.org/10.1016/S0889-5406(94)70042-7
  39. Reynolds EC : Remineralization of enamel subsurface lesions by casein phosphopeptide-stabilized calcium phosphate solutions. J Dent Res, 76:1587-95, 1997. https://doi.org/10.1177/00220345970760091101
  40. ten Cate JM : In vitro studies on the effects of fluoride on de- and remineralization. J Dent Res, 69:614-9, 1990. https://doi.org/10.1177/00220345900690S120