Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Use of Restriction Fragment Length Polymorphism Analysis to Differentiate Fungal Strains in Sunchang Meju

  • Jung, Jong-Hyun (Graduate School of Biotechnology, Institute of Life Science and Resources, KyungHee University) ;
  • Seo, Dong-Ho (Graduate School of Biotechnology, Institute of Life Science and Resources, KyungHee University) ;
  • Bhoo, Sung-Hee (Graduate School of Biotechnology, Institute of Life Science and Resources, KyungHee University) ;
  • Ha, Suk-Jin (Graduate School of Biotechnology, Institute of Life Science and Resources, KyungHee University) ;
  • Kim, Jong-Sang (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Kim, Jeong-Hwan (Department of Food Science and Technology, Gyeongsang National University) ;
  • Kwon, Dae-Young (Korea Food Research Institute) ;
  • Cha, Jae-Ho (Department of Biological Science, Pusan National University) ;
  • Park, Cheon-Seok (Graduate School of Biotechnology, Institute of Life Science and Resources, KyungHee University)
  • Published : 2008.08.31
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Abstract

Twenty-three fungal strains were isolated from meju that had originated from the Sunchang province, the famous location for making fermented soybean foods in Korea. The restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region of the rDNA (ITS-RFLP) was applied to differentiate the isolated fungal strains. First, the ITS region by polymerase chain reaction (PCR) with specific primers was amplified and then cleaved the products with different restriction enzymes. Cleavage of the amplified fragments with the restriction enzymes AluI, HaeIII, HhaI, and TaqI revealed extensive polymorphisms. The ITS-RFLP results highly correlated with ITS sequence analysis. All of the 23 fungal strains were classified into 5 groups by ITS-RFLP analysis. Aspergillus oryzae was the major fungal strain isolated from Sunchang meju (12 out of 23), while Aspergillus fumigatus was the next most frequently isolated strain (7 out of 23). In contrast, it was found that Fusarium asiaticum, Aspergillus sydowii, and Arthrinium sp. were the minor fungal strains in meju.

Keywords

References

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