Detection of Multi-class Pesticide Residues Using Surface Plasmon Resonance Based on Polyclonal Antibody

  • Yang, Gil-Mo (National Institute of Agricultural Engineering, Rural Development Administration) ;
  • Kang, Suk-Won (National Institute of Agricultural Engineering, Rural Development Administration)
  • 발행 : 2008.06.30

초록

The detection of carbamate (carbofuran, carbaryl, benfracarb, thiodicarb, and methomil) and organophosphate (diazinon, cadusafos, ethoprofos, parathion-methyl, and chlorpyrifos) pesticide residues with very low detection limits was carried out using surface plasmon resonance (SPR) based equipment. The capacity to develop a portable SPR biosensor for food safety was also investigated. The applied ligand for the immunoassays was polyclonal goat anti-rabbit immunoglobulin (IgG) peroxidase conjugate. Concentration tests using direct binding assays showed the possibility of quantitative analysis. For ligand fishing to find a proper antibody to respond to each pesticide, acetylcholinesterase (AChE), and glutathione-S-transferase (GST) were tested. The reproducibility and precision of SPR measurements were evaluated. With this approach, the limit of detection for pesticide residues was 1 ng/mL and analysis took less than 11 min. Thus, it was demonstrated that detecting multi-class pesticide residues using SPR and IgG antibodies provides enough sensitivity and speed for use in portable SPR biosensors.

키워드

참고문헌

  1. Chun MH, Lee MG. Reduction of pesticide residues in the production of red pepper powder. Food Sci. Biotechnol. 15: 57-62 (2006)
  2. Ivnitskii DM, Rishpon J. A potentiometric biosensor for pesticides based on the thiocholine hexacyanoferrate (III) reaction. Biosens. Bioelectron. 9: 569-576 (1994) https://doi.org/10.1016/0956-5663(94)80049-9
  3. Kang SM, Lee MG. Fate of some pesticides during brining and cooking of Chinese cabbage and spinach. Food Sci. Biotechnol. 14: 77-81 (2005)
  4. Pogaenik L, Mladen F. Determination of organophosphate and carbamate pesticides in spiked samples of tap water and fruit juices by a biosensor with photothermal detection. Biosens. Bioelectron. 14: 569-578 (1999) https://doi.org/10.1016/S0956-5663(99)00029-9
  5. Millard CB, Broomfield CA. Anticholinesterases: Medical applications of neurochemical principles. J. Neurochem. 64: 1909- 1918 (1995) https://doi.org/10.1046/j.1471-4159.1995.64051909.x
  6. Marty JL, Mionetto N, Lacorte S, Barcelo D. Validation of an enzymatic biosensor with various liquid chromatographic techniques for determining organophosphorus pesticides and carbaryl in freezedried waters. Anal. Chim. Acta 311: 265-271 (1995) https://doi.org/10.1016/0003-2670(94)00617-U
  7. Andreou VG, Clonis YD. Novel fiber-optic biosensor based on immunobilized glutathione S-transferase and sol-gel entrapped bromcresol green for the determination of atrazine. Anal. Chim. Acta 460: 151-161 (2002) https://doi.org/10.1016/S0003-2670(02)00250-7
  8. Dzantiev BB, Yazynina EV, Zherdev AV, Plekhanova YV, Reshetilov AN, Changc SC, McNeil CJ. Determination of the herbicide chlorsulfuron by amperometric sensor based on separation-free bienzyme immunoassay. Sensors Actuat. B-Chem. 98: 254-261 (2004) https://doi.org/10.1016/j.snb.2003.10.021
  9. Harris RD, Luff BJ, Wilkinson JS, Piehler J, Brecht A, Gauglitz G, Abuknesha RA. Integrated optical surface plasmon resonance immunoprobe for simazine detection. Biosens. Bioelectron. 14: 377- 386 (1999) https://doi.org/10.1016/S0956-5663(99)00014-7
  10. Song SJ, Cho HK. Enzyme immunoassay for on-line sensing of the insecticide imidaclopird residues. J. Korean Soc. Agric. Mach. 28: 505-510 (2003)
  11. Xing WL, Ma LR, Jiang ZH, Cao FH, Ming-Hong JC. Portable fiber-optic immunosensor for detection of methsulfuron methyl. Talanta 52: 879-883 (2000) https://doi.org/10.1016/S0039-9140(00)00440-9
  12. Oh CH. Applicability of using GC-PDD (pulsed discharge detector) for multiresidual pesticides analysis. Food Sci. Biotechnol. 15: 959- 966 (2006)
  13. Yang GM, Cho NH. Sensing of the insecticide carbofuran residues by surface plasmon resonance and immunoassay. J. Biosystems Eng. 30: 333-339 (2005) https://doi.org/10.5307/JBE.2005.30.6.333
  14. Coulet PR. Biosensor Principles and Applications. Marcel Dekker, Inc., New York, NY, USA. pp. 1-6 (1991)
  15. Marty JL, Leca B, Noguer T. Biosensors for the detection of pesticides. Analusis 26: M144-M149 (1998) https://doi.org/10.1051/analusis:199826060144
  16. Marco MP, Barceló D. Environmental applications of analytical biosensors. Meas. Sci. Technol. 7: 1547-1562 (1996) https://doi.org/10.1088/0957-0233/7/11/002
  17. Maurize E, Calle A, Lechuga LM, Quintana J, Montoya A, Manclús JJ. Real-time detection of chlorpyrifos at part per trillion levels in ground, surface, and drinking water samples by a portable surface plasmon resonance immunosensor. Anal. Chim. Acta 561: 40-47 (2006) https://doi.org/10.1016/j.aca.2005.12.069
  18. Mauriz E, Calle A, Abad A, Montoya A, Hildebrandt A, Barcelo D, Lechuga LM. Determination of carbaryl in natural water samples by a surface plasmon resonance flow-through immunosensor. Biosens. Bioelectron. 21: 2129-2136 (2006) https://doi.org/10.1016/j.bios.2005.10.013
  19. Wilson M, Nakane P. Immunofluorescence and Related Staining Techniques. Elsevier/North Holland BioMedical Press, Amsterdam, Netherlands. p. 215 (1978)
  20. Mouvet C, Broussard S, Jeannot R, Maciag C, Abuknesha R, Ismail G. Validation of commercially available ELISA microtiter plates for triazines in water samples. Anal. Chim. Acta 311: 331-339 (1995) https://doi.org/10.1016/0003-2670(94)00638-3
  21. Mouvet C, Amalric L, Broussard S, Lang G, Brecht A, Gauglitz G. Reflectometric interference spectroscopy for the determination of atrazine in natural water samples. Environ. Sci. Technol. 30: 1846- 1851 (1996) https://doi.org/10.1021/es9503894
  22. Quinn JG, O'Kennedy R. Transduction platforms and biointerfacial design of biosensors for 'real-time' biomolecular interaction analysis. Anal. Lett. 32: 1475-1517 (1999) https://doi.org/10.1080/00032719908542911
  23. Stephen JD, Gary JK, Paul PD, Bernadette MM, Richard O, Heather AL, Michael RA. Development of surface plasmon resonance-based immunoassay for aflatoxin B1. J. Agr. Food Chem. 48: 5097-5104 (2000) https://doi.org/10.1021/jf9911693
  24. Stenberg E, Persson B, Roos H, Urbaniczky C. Quantitative determination of surface concentration of protein with surface plasmon resonance using radiolabeled proteins. J. Colloid. Interf. Sci. 143: 513-526 (1991) https://doi.org/10.1016/0021-9797(91)90284-F
  25. Malmborg AC, Borrebaeck CA. BIAcore as a tool in antibody engineering. J. Immunol. Methods 183: 7-13 (1995) https://doi.org/10.1016/0022-1759(95)00018-6
  26. Aga DS, Thurman EM. Immunochemical Technology for Environmental Applications. American Chemical Society ACS Symposium Series 657, American Chemical Society, Washington DC, USA. pp. 22-23 (1997)
  27. Elwing H. Protein absorption and ellipsometry in biomaterial research. Biomaterials 19: 397-406 (1998) https://doi.org/10.1016/S0142-9612(97)00112-9
  28. Macromolecular interactions facility (MIF). Ligand fishing. Available from: http:// www.med.unc.edu/wrkunits/2depts/biochem/ MACINFAC/biacore.html#10. Accessed Mar. 5, 2007
  29. Drewianka S. Biochips in drug development. Available from: http:// www.innovations-report.de. Accessed Mar. 5, 2007
  30. David W. The Immunoassay Handbook. 5th ed. Elsevier Press. Oxford. UK. pp. 289-291 (2005)
  31. Zhu H, Snyder M. Protein chip technology. Curr. Opin. Chem. Biol. 7: 55-63 (2003) https://doi.org/10.1016/S1367-5931(02)00005-4
  32. McDonnell JM. Surface plasmon resonance: Towards an understanding of the mechanism of biological molecular recognition. Curr. Opin. Chem. Biol. 5: 572-577 (2001) https://doi.org/10.1016/S1367-5931(00)00251-9
  33. Yuk JS, Ha KS. Proteomic applications of surface plasmon resonance biosensors: Analysis of protein arrays. Exp. Mol. Med. 37: 1-10 (2005) https://doi.org/10.1038/emm.2005.1