Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Analysis of Housekeeping Genes in Mice Feeding on GM and non-GM Potatoes

해충저항성 GM감자와 non-GM감자의 Housekeeping gene 발현 분석

  • Kweon, Mi-Ae (Department of Food Science & Technology, Kyungpook National University) ;
  • Heo, Jin-Chul (Department of Food Science & Technology, Kyungpook National University) ;
  • Cho, Hyun-Seok (National Institute of Agricultural Biotechnology, RDA) ;
  • Lee, Sang-Han (Department of Food Science & Technology, Kyungpook National University)
  • 권미애 (경북대학교 식품공학과) ;
  • 허진철 (경북대학교 식품공학과) ;
  • 조현석 (농진청 농업생명공학연구원 생물안전성과) ;
  • 이상한 (경북대학교 식품공학과)
  • Published : 2008.08.30
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Abstract

To develop human risk assessment protocols, we explored housekeeping gene and cytokine expression in mouse spleen cells using Rt-PCR. We normalized housekeeping gene expression by RT-PCR; gene expression was highly uniform in potato leafs and mouse spleen cells. We measured the expression of frequently used housekeeping genes, such as those encoding APRT, $\beta$-tubulin, Actin, Hsp 20.2, Cyclophilin, 18S RNA, Efla, Tbp, GAPDH, $\beta$-actin, Tuba2, Hprt, Cyclophlin A, Tfrc, and RPL13A in mice fed GM or non-GM potatoes. Housekeeping gene expression did not show any significant differences between GM and non-GM potato-fed mice. The murine model of potato-fed mice did not express IL-4 and IL-13 at a significant levels.

GM 및 non-GM 감자의 인체 위해성 여부를 판단하기 위하여 마우스 비장세포에서 인터루킨과 housekeeping gene의 발현을 RT-PCR로 비교 분석하였다. 유전자변형 감자의 잎과 이를 섭취한 마우스의 비장세포에서 발현되는 housekeeping gene의 발현을 수행한 결과, APRT, $\beta$-tubulin, Actin, Hsp 20.2, Cyclophilin, 18S RNA and Efla, and Tbp, GAPDH, $\beta$-actin, Tuba2, Hprt, Cyclophlin A, Tfrc, and RPL13A의 발현에는 유전자 변형 감자와 그렇지 않은 감자와의 차이를 발견할 수 없었다. GM작물의 안전성에 대한 연구는 많은 표본을 가지고, 보다 장기적인 관점에서 다양한 모니터링 시스템을 도입이 필요하다고 판단되며 이의 향후 연구에 많은 관심이 필요하다.

Keywords

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