Comparative Biodegradation of HDPE and LDPE Using an Indigenously Developed Microbial Consortium

  • Satlewal, Alok (Department of Microbiology, C.B.S.H., G.B. Pant University of Agriculture and Technology) ;
  • Soni, Ravindra (Department of Microbiology, C.B.S.H., G.B. Pant University of Agriculture and Technology) ;
  • Zaidi, Mgh (Department of Chemistry, C.B.S.H., G.B. Pant University of Agriculture and Technology) ;
  • Shouche, Yogesh (National Centre for Cell Sciences, Pune University Campus) ;
  • Goel, Reeta (Department of Microbiology, C.B.S.H., G.B. Pant University of Agriculture and Technology)
  • Published : 2008.03.31

Abstract

A variety of bacterial strains were isolated from waste disposal sites of Uttaranchal, India, and some from artificially developed soil beds containing maleic anhydride, glucose, and small pieces of polyethylene. Primary screening of isolates was done based on their ability to utilize high- and low-density polyethylenes (HDPE/LDPE) as a primary carbon source. Thereafter, a consortium was developed using potential strains. Furthermore, a biodegradation assay was carried out in 500-ml flasks containing minimal broth (250ml) and HDPE/LDPE at 5mg/ml concentration. After incubation for two weeks, degraded samples were recovered through filtration and subsequent evaporation. Fourier transform infrared spectroscopy (FTIR) and simultaneous thermogravimetric-differential thermogravimetry-differential thermal analysis (TG-DTG-DTA) were used to analyze these samples. Results showed that consortium-treated HDPE (considered to be more inert relative to LDPE) was degraded to a greater extent (22.41% weight loss) in comparison with LDPE (21.70% weight loss), whereas, in the case of untreated samples, weight loss was more for LDPE than HDPE (4.5% and 2.5%, respectively) at $400^{\circ}C$. Therefore, this study suggests that polyethylene could be degraded by utilizing microbial consortia in an eco-friendly manner.

Keywords

References

  1. Albertsson, A. C., B. Erlandsson, M. Hakkarainen, and S. Karlsson. 1998. Molecular weight changes and polymeric matrix changes correlated with the formation of degradation products in biodegraded polyethylene. J. Environ. Polym. Degrad. 6: 187-195 https://doi.org/10.1023/A:1021873631162
  2. Brown, B. S., J. Mills, and J. M. Hulse. 1974. Chemical and biological degradation of plastics. Nature 250: 161-163 https://doi.org/10.1038/250161a0
  3. Cornell, J. H., A. M. Kaplan, and M. R. Rogers. 1984. Biodegradation of photooxidized polyalkylenes. J. Appl. Polym. Sci. 29: 2581-2597 https://doi.org/10.1002/app.1984.070290814
  4. Dua, N. 2004. Honey who shrunk the plastic, pp. 22. In: Down to Earth. CSE, India
  5. Hadad, D., S. Geresh, and A. Sivan. 2005. Biodegradation of polyethylene by the thermophilic bacterium Brevibacillus borstelensis. J. Appl. Microbiol. 98: 1093-1100 https://doi.org/10.1111/j.1365-2672.2005.02553.x
  6. Orhan, Y. and H. Buyukgungor. 2000. Enhancement of biodegradability of disposable polyethylene in controlled biological soil. Int. Biodeterior. Biodegrad. 45: 49-55 https://doi.org/10.1016/S0964-8305(00)00048-2
  7. Otake, Y., T. Kobayashi, H. Ashabe, N. Murakami, and K. Ono. 1995. Biodegradation of low-density polyethylene, polystyrene, polyvinyl-chloride, and urea-formaldehyde resin buried under soil for over 32 years. J. Appl. Polym. Sci. 56: 1789-1796 https://doi.org/10.1002/app.1995.070561309
  8. Potts, J. E. 1978. Biodegradation, pp. 617-658. In H. H. G. Jelinek (ed.), Aspect of Degradation and Stabilization of Polymers. Elsevier, New York
  9. Volke-Sepulveda, T., G. Saucedo-Castaneda, M. Gutierrez-Rojas, A. Manzur, and E. Favela-Torres. 2002. Thermally treated low density polyethylene biodegradation by Penicillium pinophilum and Aspergillus niger. J. Appl. Polym. Sci. 83: 305-314 https://doi.org/10.1002/app.2245