Thermostable ${\beta}$-Glycosidase-CBD Fusion Protein for Biochemical Analysis of Cotton Scouring Efficiency

  • 발행 : 2008.03.31

초록

Multidomain proteins for the biochemical analysis of the scouring efficiency of cotton fabrics were constructed by the fusion of a reporter moiety in the N-terminal and the cellulose binding domain (CBD) in the C-terminal. Based on the specific binding of the CBD of Cellulomonas fimi exoglucanase (Cex) to crystalline cellulose (Avicel), the reporter protein is guided to the cellulose fibers that are increasingly exposed as the scouring process proceeds. Among the tested reporter proteins, a thermostable ${\beta}$-glycosidase (BglA) from Thermus caldophilus was found to be most appropriate, showing a higher applicability and stability than GFP, DsRed2, or a tetrameric ${\beta}$-glycosidase (GUS) from Escherichia coli, which were precipitated more seriously during the expression and purification steps. When cotton fabrics with different scouring levels were treated with the BglA-CBD and incubated with X-Gal as the chromogenic substrate, an indigo color became visible within 2 h, and the color depth changed according to the conditions and extent of the scouring.

키워드

참고문헌

  1. AATCC. AATCC test method 39-1980. In: AATCC Test Manual. pp. 55: 286
  2. Calafell, M. and P. Garriga. 2004. Effect of some process parameters in the enzymatic scouring cotton using an acid pectinase. Enzyme Microb. Technol. 34: 326-331 https://doi.org/10.1016/j.enzmictec.2003.11.008
  3. Carrard, G., A. Koivula, H. Soderlund, and P. Beguin. 2000. Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose. Proc. Natl. Acad Sci. USA 97: 10342-10347
  4. Choi, J. J., E. J. Oh, Y. J. Lee, D. S. Suh, J. H. Lee, S. W. Lee, H. T. Shin, and S. T. Kwon. 2003. Enhanced expression of the gene for $\beta$-glycosidase of Thermus caldophilus GK24 and synthesis of galacto-oligosaccharides by the enzyme. Biotechnol. Appl. Biochem. 38: 131-136 https://doi.org/10.1042/BA20020119
  5. Chung, H. Y., E. J. Yang, and H. C. Chang. 2006. Substitutions for Cys-472 and His-509 at the active site of $\beta$-galactosidase from Lactococcus lactis ssp. lactis 7962 cause large decreases in enzyme activity. J. Microbiol. Biotechnol. 16: 1325-1329
  6. Degani, O., S. Gepstein, and C. G. Dosoretz. 2004. A new method for measuring scouring efficiency of natural fibers based on the cellulose-binding domain $\beta$-glucuronidase fused protein. J. Biotechnol. 107: 265-273 https://doi.org/10.1016/j.jbiotec.2003.10.015
  7. Edberg, S. C. and C. M. Kontnick. 1986. Comparison of $\beta$-glucuronidase-based substrate systems for identification of Escherichia coli. J. Clin. Microbiol. 24: 368-371
  8. Gilkes, N. R., D. G. Kilburn, J. R. C. Miller, and R. A. Warren. 1989. Structural and functional analysis of a bacterial cellulase by proteolysis. J. Biol. Chem. 264: 17802-17808
  9. Griffith, K. L. and J. R. E. Wolf. 2002. Measuring $\beta$-galactosidase activity in bacteria: Cell growth, permeabilization, and enzyme assay in 96-well arrays. Biochem. Biophys. Res. Commun. 290: 397-402 https://doi.org/10.1006/bbrc.2001.6152
  10. Hartzell, M. M. and Y. L. Hsieh. 1998. Enzymatic scouring to improve cotton fabric wettability. Textile Res. J. 68: 233-241 https://doi.org/10.1177/004051759806800401
  11. Hsieh, Y. L., J. Thompson, and A. Miller. 1996. Water wetting and retention properties of cotton assemblies as affected by alkaline and bleaching treatments. Textile Res. J. 66: 456-464 https://doi.org/10.1177/004051759606600707
  12. Judd, D. B. and G. Wysezcki. 1975. Color in Business, Science and Industry. New York, John Wiley & Sons
  13. Karmakar, S. R. 1998. Application of biotechnology in the pretreatment processes of textiles. Colourage Annual: 75-86
  14. Kim, D. Y., E. Rha, S. L. Choi, J. J. Song, S. P. Hong, M. H. Sung, and S. G. Lee. 2007. Development of bioreactor system for L-tyrosine synthesis using thermostable tyrosine phenollyase. J. Microbiol. Biotechnol. 17: 116-122
  15. Kim, J., S. Y. Kim, and E. K. Choe. 2007. The beneficial influence of enzymatic scouring in cotton properties. J. Natural Fibers 2: 39-52
  16. Kim, J. H., J. J. Song, B. G. Kim, M. H. Sung, and S. C. Lee. 2004. Enhanced stability of tyrosine phenol-lyase from Symbiobacterium toebii by DNA shuffling. J. Microbiol. Biotechnol. 14: 153-157
  17. Lee, S. G., S. P. Hong, D. Y. Kim, J. J. Song, H. S. Ro, and M. H. Sung. 2006. Inactivation of tyrosine phenol-lyase by Pictet-Spengler Reaction and alleviation by T15A mutation on intertwined N-terminal arm. FEBS J. 273: 5564-5573 https://doi.org/10.1111/j.1742-4658.2006.05546.x
  18. Levy, I. and O. Shoseyov. 2000. Cellulose-binding domains: Biotechnological applications. Biotechnol. Adv. 20: 191-213 https://doi.org/10.1016/S0734-9750(02)00006-X
  19. Li, Y. and I. R. Hardin. 1998. Treating cotton with cellulases and pectinase: Effects on cuticle properties. Textile Res. J. 68: 671-679 https://doi.org/10.1177/004051759806800908
  20. Lin, C. H. and Y. L. Hsieh. 2001. Direct scouring of greige cotton fabrics with protease. Textile Res. J. 71: 425-434 https://doi.org/10.1177/004051750107100510
  21. Nikolova, P. V., A. L. Creagh, S. J. Duff, and C. A. Haynes. 1997. Thermostability and irreversible activity loss of exoglucanase/xylanase Cex from Cellulomonas fimi. Biochemistry 36: 1381-1388 https://doi.org/10.1021/bi962367f
  22. O'Neill, G., S. H. Goh, R. A. Warren, D. G. Kilburn, and R. C. J. Miller. 1986. Structure of the gene encoding the exoglucanase of Cellulomonas fimi. Gene 44: 325-330 https://doi.org/10.1016/0378-1119(86)90197-6
  23. Shoseyov, O. and R. A. Warren. 1997. Cellulose binding domains - a novel fusion technology for efficient, low cost purification and immobilization of recombinant proteins. inNovations 7: 1-3
  24. Tomme, P., A. Boraston, B. McLean, J. Kormos, A. L. Creagh, K. Sturch, N. R. Gilkes, C. A. Haynes, R. A. Warren, and D. G. Kilburn. 1998. Characterization and affinity applications of cellulose-binding domains. J. Chromatogr. B Biomed. Sci. Appl. 715: 283-296 https://doi.org/10.1016/S0378-4347(98)00053-X
  25. Tzanov, T., M. Calafell, G. M. Guebitz, and A. Cavaco-Paulo. 2001. Bio-preparation of cotton fabrics. Enzyme Microb. Technol. 29: 357-362 https://doi.org/10.1016/S0141-0229(01)00388-X
  26. Xiao, Z., P. Gao, Y. Qu, and T. Wang. 2001. Cellulose-binding domain of endoglucanase III from Trichoderma reesei disrupting the structure of cellulose. Biotechnol. Lett. 23: 711-715 https://doi.org/10.1023/A:1010325122851