Kinetics of Cell Growth and Cyclosporin A Production by Tolypocladium inflatum when Scaling Up from Shake Flask to Bioreactor

  • El Enshasy, H. (Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications) ;
  • Fattah, Y. Abdel (Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications) ;
  • Atta, A. (Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications) ;
  • Anwar, M. (Microbiology Department, Faculty of Pharmacy, Alexandria University) ;
  • Omar, H. (Microbiology Department, Faculty of Pharmacy, Alexandria University) ;
  • Magd, S. Abou El (Microbiology Department, Faculty of Pharmacy, Alexandria University) ;
  • Zahra, R. Abou (Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications)
  • Published : 2008.01.31

Abstract

The kinetics of cell growth and Cyclosporin A (Cyc A) production by Tolypocladium inflatum were studied in shake flasks and bioreactors under controlled and uncontrolled pH conditions. In the case of the shake flask, the production time was extended to 226 h and the maximal antibiotic concentration was 76 mg/l. When scaling up the cultivation process to a bioreactor level, the production time was reduced to only 70h with a significant increase in both the cell growth and the antibiotic production. The maximal dry cell weights in the case of the controlled pH and uncontrolled pH cultures in the bioreactor were 22.4g/l and 14.2g/l, respectively. The corresponding maximal dry cell weight values did not exceed 7.25g/l with the shake flask cultures. The maximal values for Cyc A production were 144.72 and 131.4 mg/l for the controlled and uncontrolled pH cultures, respectively. It is also worth noting that a significant reduction was observed in both the dry cell mass and the antibiotic concentration after the Cyc A production phase, whereas the highest rate of antibiotic degradation was observed in the stirred tank bioreactor with an uncontrolled pH. Morphological characterization of the micromorphological cell growth (mycelial/pellet forms) was also performed during cultivation in the bioreactor.

Keywords

References

  1. Agathos, S. N., J. W. Marshall, C. Moraiti, R. Parekh, and C. Madhosingh. 1986. Physiological and genetic factors for process development of cyclosporine fermentations. J. Ind. Microbiol. 1: 39-48 https://doi.org/10.1007/BF01569415
  2. Alfonso, C., L. Cribiero, and F. Reyes. 1989. Penicillin amidohydrolases in fungal autolysis. Microbiol. Immunol. 33: 69-74 https://doi.org/10.1111/j.1348-0421.1989.tb01498.x
  3. Balakrishnan, K. and A. Pandey. 1996. Influence of amino acids on the biosynthesis of cyclosporine A by Tolypocladium inflatum. Appl. Microbiol. Biotechnol. 45: 800-803 https://doi.org/10.1007/s002530050765
  4. Buchs, J. 2001. Introduction to advantages and problems of shaken cultures. J. Biochem. Eng. 7: 91-98 https://doi.org/10.1016/S1369-703X(00)00106-6
  5. Chun, G.-T. and S. N. Agathos. 1991. Comparative studies of physiological and environmental effects on the production of cyclosporine A in suspended and immobilized cells of Tolypocladium inflatum. Biotechnol. Bioeng. 37: 256-265 https://doi.org/10.1002/bit.260370308
  6. Dubois, M., K. Gilles, J. Hamilton, P. Rebers, and F. Smith. 1956. Colorimetric method of determination of sugars and related substances. Anal. Chem. 290: 181-186
  7. El Enshasy, H. 2007. Filamentous fungal cultures - process characteristics, products, and applications, pp. 225-261. In S. T. Yang (ed.), Bioprocessing for Value-added Products from Renewable Resources. Elsevier Press, The Netherlands
  8. Harvey, L. M., B. McNeil, D. R. Berry, and S. White. 1998. Autolysis in batch cultures of Penicillium chrysogenum at varying agitation rates. Enz. Microb. Technol. 22: 446-458 https://doi.org/10.1016/S0141-0229(97)00234-2
  9. Heydarian, S. M., M. D. Lilly, and A. P. Ison. 1996. The effect of culture conditions on the production of erythromycin by Saccharopolyspora erythraea in batch culture. Biotechnol. Lett. 18: 1181-1186 https://doi.org/10.1007/BF00128589
  10. Isaac, C. C., A. Jones, and M. A. Pickard. 1990. Production of cyclosporins by Tolypocladium niveum strains. Antimicrob. Agents Chemother. 34: 121-127 https://doi.org/10.1128/AAC.34.1.121
  11. Kreuzig, F. 1984. High speed liquid chromatography with conventional instruments for determination of cyclosporin A, B, C, and D in fermentation broth. J. Chromatogr. 290: 181-186 https://doi.org/10.1016/S0021-9673(01)93572-1
  12. Lee, J. and S. N. Agathos. 1991. Dynamic of L-valine in relation to the production of cyclosporine A by Tolypocladium inflatum. Appl. Microbiol. Biotechnol. 34: 513-517
  13. Lee, T. H., G.-K. Chun, and Y. K. Chang. 1997. Development of sporulation/immobilization method and its application for the continuous production of cyclosporine A by Tolypocladium inflatum. Biotechnol. Prog. 13: 546-550 https://doi.org/10.1021/bp970069j
  14. Maier, U. and J. Büchs. 2001. Characterisation of the gas-liquid mass transfer in shaking bioreactors. J. Biochem. Eng. 7: 99-106 https://doi.org/10.1016/S1369-703X(00)00107-8
  15. Martin, J. F. and A. L. Demain. 1980. Control of antibiotic biosynthesis. Microbiol. Rev. 44: 230-251
  16. Mayer, A. F. and W.-D. Deckwer. 1996. Simultaneous production and decomposition of clavulanic acid during Streptomyces clavuligerus cultivations. Appl. Microbiol. Biotechnol. 45: 41-46 https://doi.org/10.1007/s002530050646
  17. McIntyre, M., D. R. Berry, and B. McNeil. 2000. Role of protease in autolysis of Penicillium chrysogenum chemostat cultures in response to nutrient depletion. Appl. Microbiol. Biotechnol. 53: 235-242 https://doi.org/10.1007/s002530050014
  18. Murthy, M. V., E. V. Mohand, and A. K. Sadhukhan. 1999. Cyclosporin-A production by Tolypocladium inflatum using solid state fermentation. Process Biochem. 34: 269-280 https://doi.org/10.1016/S0032-9592(98)00095-8
  19. Papagianni, M. 2004. Fungal morphology and metabolite production in submerged mycelial processes. Biotechnol. Adv. 22: 189-259 https://doi.org/10.1016/j.biotechadv.2003.09.005
  20. Pritchard, D. I. 2005. Sourcing a chemical succession for cyclosporin from parasites and human pathogens. Drug Discovery Today 10: 688-691 https://doi.org/10.1016/S1359-6446(05)03395-7
  21. Schreiber, S. L. and G. R. Crabtree. 1992. The mechanism of action of cyclosporin A and FK506. Immunol. Today 13: 136-142 https://doi.org/10.1016/0167-5699(92)90111-J
  22. Sekar, C. and K. Balaraman. 1998. Immobilization of the fungus Tolypocladium sp. for the production of cyclosporin A. Bioprocess Eng. 19: 281-283
  23. Sekar, C. and K. Balaraman. 1998. Optimization studies on the production of cyclosporine A by solid state fermentation. Bioprocess Eng. 18: 293-296 https://doi.org/10.1007/s004490050444
  24. Sowden, J. M. and B. R. Allen. 1992. Cyclosporin in dermatology: A historical overview. Int. J. Dermatol. 31: 520-523 https://doi.org/10.1111/j.1365-4362.1992.tb02708.x
  25. Sugiyama, M., S. Mizuno, Y. Ohta, H. Mochizuki, and O. Nimi. 1990. Kinetic studies of streptomycin uptake implicated in selfresistance in a streptomycin producer. Biotechnol. Lett. 12: 1-6 https://doi.org/10.1007/BF01028483
  26. Szabo, I., A. Penyige, G. Barabas, and J. Barabas. 1990. Effect of aminoglycoside antibiotics on the autolytic enzyme of Streptomyces griseus. Arch. Microbiol. 155: 99-102 https://doi.org/10.1007/BF00291282
  27. Tamura, S., Y. Park, M. Toriyama, and M. Okabe. 1997. Change of mycelial morphology in tylosin production by batch culture of Streptomyces fradiae under various shear conditions. J. Ferment. Bioeng. 83: 523-528 https://doi.org/10.1016/S0922-338X(97)81131-2
  28. White, S., M. McIntyre, D. Berry, and B. McNeil. 2002. The autolysis of industrial filamentous fungi. Crit. Rev. Biotechnol. 22: 1-14 https://doi.org/10.1080/07388550290789432
  29. Wittler, R., H. Baumgartl, D. W. Lubbers, and K. Schügerl. 1986. Investigations of oxygen transfer into Penicillium chrysogenum pellets by microprobe measurement. Biotechnol. Bioeng. 28: 1024-1031 https://doi.org/10.1002/bit.260280713