Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Morphological Variation of Enterobacter sp. BL-2 in Acetate-mediated pH Environment for Excretive Production of Cationic Microbial Polyglucosamine Biopolymer

  • Son, Mi-Kyung (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Hong, Soo-Jung (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • SaGong, Kuk-Hwa (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Yong-Hyun (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University)
  • Published : 2008.01.31
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Abstract

Enterobacter sp. BL-2 excretively produced a unique cationic polyglucosamine biopolymer PGB-1 comprised of more than 95% D-glucosamine in an acetate-mediated culture condition. The excretion of the biopolymer PGB-1 was closely associated with the cellular morphology of Enterobacter sp. BL-2, a feature highly dependable on the pH of the medium. The initially formed uneven and irregular surface cells were aggregated into the cell-biopolymer network structure connected by the adhesion modules of the cell-bound biopolymer. The excretive production of the biopolymer PGB-1 coincided with the disruption of the cell-biopolymer network, most actively at the medium pH of 8.0.

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References

  1. Barua, S., T. Yamashino, T. Hasegawa, K. Yokoyama, K. Torii, and M. Ohta. 2002. Involvement of surface polysaccharides in the organic acid resistance of Shiga toxin-producing Escherichia coli O157:H7. Mol. Microbiol. 43: 629-640 https://doi.org/10.1046/j.1365-2958.2002.02768.x
  2. Fujita, M., M. Ike, S. Tachibana, G. Kitada, S. M. Kim, and Z. Inoue. 2000. Characterization of a bioflocculant produced by Citrobacter sp. TKF04 from acetic and propionic acids. J. Biosci. Bioeng. 89: 40-46 https://doi.org/10.1016/S1389-1723(00)88048-2
  3. Jang, J. H., H. C. Hia, M. Ike, C. Inoue, M. Fujita, and T. Yoshida. 2005. Acid hydrolysis and quantitative determination of total hexosamines of an exopolysaccharide produced by Citrobacter sp. Biotechnol. Lett. 27: 13-18 https://doi.org/10.1007/s10529-004-6305-y
  4. Jang, J. H., M. Ike, S. M. Kim, and M. Fujita. 2001. Production of a novel bioflocculant by fed-batch culture of Citrobacter sp. Biotechnol. Lett. 23: 593-597 https://doi.org/10.1023/A:1010312607171
  5. Kim, L. S., S. J. Hong, M. K. Son, and Y. H. Lee. 2006. Polymeric and compositional properties of novel extracellular microbial polyglucosamine biopolymer from new strain of Citrobacter sp. BL-4. Biotechnol. Lett. 28: 241-245 https://doi.org/10.1007/s10529-005-5525-0
  6. Peterson, A. A., R. E. Hancock, and E. J. McGroarty. 1985. Binding of polycationic antibiotics and polyamines to lipopolysaccharides of Pseudomonas aeruginosa. J. Bacteriol. 164: 1256-1261
  7. Son, M. K., H. D. Shin, T. L. Huh, J. H. Jang, and Y. H. Lee. 2005. Novel cationic microbial polyglucosamine biopolymer from new Enterobacter sp. BL-2 and its bioflocculation efficacy. J. Microbiol. Biotechnol. 15: 626-632
  8. Wilson, W. W., M. M. Wade, S. C. Holman, and F. R. Champlin. 2001. Status of methods for assessing bacterial cell surface charge properties based on zeta potential measurements. J. Microbiol. Methods 43: 153-164 https://doi.org/10.1016/S0167-7012(00)00224-4