Journal of Microbiology and Biotechnology

  • 제18권1호
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  • Pages.74-79
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  • 2008
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Chemical Composition and Biological Activities of Essential Oils Extracted from Korean Endemic Citrus Species

  • Baik, Jong-Seok (Department of Chemistry, Cheju National University) ;
  • Kim, Sang-Suk (SkinCure Consmetics Inc., JeJu Bio-Industry Development Center) ;
  • Lee, Jung-A (Laboratory of Microbiology and Biotechnology, Jeju Biodiversity Research Institute(JBRI)) ;
  • Oh, Tae-Heon (Department of Chemistry, Cheju National University) ;
  • Kim, Ji-Young (Laboratory of Microbiology and Biotechnology, Jeju Biodiversity Research Institute(JBRI)) ;
  • Lee, Nam-Ho (Department of Chemistry, Cheju National University) ;
  • Hyun, Chang-Gu (Laboratory of Microbiology and Biotechnology, Jeju Biodiversity Research Institute(JBRI))
  • 발행 : 2008.01.31
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초록

The aim of this study was to analyze the chemical composition of 14 kinds of citrus oils and to test their biological activities. Citrus essential oils were obtained by steam distillation from immature fruits collected from Jeju Island and were analyzed using gas chromatograph (GC)-flame ionization detectors (FID) and GC-MS. Limonene (55.4% to 91.7%), myrcene (2.1% to 32.1%), ${\alpha}$-pinene (0.6% to 1.6%) and linalool (0.4% to 6.9%) were the major components in most citrus species. To evaluate in vitro antibacterial activity, all essential oils were tested against Propionibacterium acnes and Staphylococcus epidermidis. Nine out of fourteen citrus oils exhibited antibacterial activity against P. acnes, but not against S. epidermidis. The effects of the citrus oils on DPPH radical scavenging, superoxide radical anion scavenging, nitric oxide radical, and cytotoxicity were also assessed. Three essential citrus oils, Joadeung, Dongjunggyul, and Bujiwha, exhibited potent inhibitory effects on nitric oxide production. Two essential oils, Dongjunggyul and Joadeung, showed potent free radical scavenging activities in the DPPH assay. For future applications in cosmetic products, we also performed MTT assays in a human dermal fibroblast cell line. The majority of the essential oils showed no cytotoxicity. The results indicate that citrus essential oils can be useful natural agents for cosmetic application.

키워드

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