Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular Diversity of Bacterial Communities from Subseafloor Rock Samples in a Deep-Water Production Basin in Brazil

  • Von Der Weid, Irene (Instituto de Microbiologia Prof. Paulo de Goes, UFRJ, CCS, Bloco I, Ilha do Fundao) ;
  • Korenblum, Elisa (Instituto de Microbiologia Prof. Paulo de Goes, UFRJ, CCS, Bloco I, Ilha do Fundao) ;
  • Jurelevicius, Diogo (Instituto de Microbiologia Prof. Paulo de Goes, UFRJ, CCS, Bloco I, Ilha do Fundao) ;
  • Rosado, Alexandre Soares (Instituto de Microbiologia Prof. Paulo de Goes, UFRJ, CCS, Bloco I, Ilha do Fundao) ;
  • Dino, Rodolfo (CENPES, PETROBRAS, Ilha do Fundao) ;
  • Sebastian, Gina Vasquez (CENPES, PETROBRAS, Ilha do Fundao) ;
  • Seldin, Lucy (Instituto de Microbiologia Prof. Paulo de Goes, UFRJ, CCS, Bloco I, Ilha do Fundao)
  • Published : 2008.01.31
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Abstract

The deep subseafloor rock in oil reservoirs represents a unique environment in which a high oil contamination and a very low biomass can be observed. Sampling this environment has been a challenge owing to the techniques used for drilling and coring. In this study, the facilities developed by the Brazilian oil company PETROBRAS for accessing deep subsurface oil reservoirs were used to obtain rock samples at 2,822-2,828 m below the ocean floor surface from a virgin field located in the Atlantic Ocean, Rio de Janeiro. To address the bacterial diversity of these rock samples, PCR amplicons were obtained using the DNA from four core sections and universal primers for 16S rRNA and for APS reductase (aps) genes. Clone libraries were generated from these PCR fragments and 87 clones were sequenced. The phylogenetic analyses of the 16S rDNA clone libraries showed a wide distribution of types in the domain bacteria in the four core samples, and the majority of the clones were identified as belonging to Betaproteobacteria. The sulfate-reducing bacteria community could only be amplified by PCR in one sample, and all clones were identified as belonging to Gammaproteobacteria. For the first time, the bacterial community was assessed in such a deep subsurface environment.

Keywords

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