Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Suppression of Bacterial Wilt with Bacillus subtilis SKU48-2 Strain

Bacillus subtilis SKU48-2에 의한 풋마름병 발병 억제

  • Kim, Ji-Tae (Department of Biological Engineering, Seokyeong University) ;
  • Kim, Shin-Duk (Department of Biological Engineering, Seokyeong University)
  • Published : 2008.06.28
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Abstract

Bacterial populations from the rhizosphere were obtained and the efficacy of the bacterial wilt suppression, root colonizing ability and resistance to three kinds of chemical pesticides were assayed. According to these results, SKU48-2 was selected as a potential biological agent to control the bacterial wilt caused by Ralstonia solanacearum. SKU48-2 strain at $10^8CFU/ml$ inoculum was able to suppress the bacterial wilt up to 60% in greenhouse trials. Also, the resistance of SKU48-2 to chemical pesticides make possible to use in combination with chemical pesticides for the control of bacterial wilt. Three different powder formulations of SKU48-2 were developed. The shelf-life of powder formulations was effective up to 6 months of storage. Unformulated bacterial suspension could not be stored for 2 weeks, at which time cell viability was completely lost. According to 16S rDNA sequence data, the SKU48-2 stain was identified as Bacillus subtilis.

근권 미생물을 대상으로 뿌리에서의 집락 형성 실험, 풋마름병 방제 효과 그리고 화학 농약에 대한 내성을 검정하여 SKU48-2 균주를 선발하였다. SKU48-2균주는 균체 현탁액$(10^8CFU/ml)$을 처리하였을 때 60%이상의 풋마름병 방제 효과를 나타내었으며, 형태적, 생화학적 특성과 16S rDNA sequence 분석 결과 Bacillus subtilis SKU48-2로 동정되었다. 선발 균주 SKU48-2는 화학 농약에 대해 강한 내성을 가져 토양 내 잔류 농약에 의해 영향을 받지 않을 뿐만 아니라, 화학 농약과의 혼용에 의해서 저농약 사용이 가능하고 미생물제제 단독 사용 시 보다 높은 방제 효과를 기대할 수 있다. SKU 48-2균주가 액상의 배양액 상태에서는 4주 만에 생균밀도가 급격히 감소한 반면, talc, vermiculite와 peat등의 담체 사용 시는 생균밀도가 6개월 간 유지됨이 확인되어 고형 제제화의 기초를 마련하였다.

Keywords

References

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