한국 재래종 차나무(Camellia sinensis)의 작물학적 특성 및 품질관련 성분 변이

Variation of Growth Characteristics and Quality Related Components in Korean Indigenous Tea (Camellia sinensis) Germplasms

  • 이민석 (아모레퍼시픽 설록차 연구소) ;
  • 이진호 (아모레퍼시픽 설록차 연구소) ;
  • 이정대 (아모레퍼시픽 설록차 연구소) ;
  • 현진욱 (아모레퍼시픽 설록차 연구소) ;
  • 김영걸 (아모레퍼시픽 설록차 연구소) ;
  • 황영선 (강원대학교 생약자원개발학과) ;
  • 이현진 (강원대학교 생약자원개발학과) ;
  • 최수산나 (강원대학교 생약자원개발학과) ;
  • 이수진 (강원대학교 생약자원개발학과) ;
  • 정명근 (강원대학교 생약자원개발학과)
  • 발행 : 2008.09.30

초록

국내 수집 보존 중인 재래종 차나무 유전자원 238 계통의 작물학적 특성 및 품질관련 성분을 조사하여 우리나라 재래 유전자원을 활용한 육종소재용 우수 모수 선발 및 고 기능성차 신제품 개발을 위한 기초 자료를 얻고자 수행된 연구 결과를 요약하면 다음과 같다. 1. 국내 수집 차나무 유전자원 238 계통을 대상으로 작물학적 특성 중 맹아기의 조만성을 비교한 결과 조생종이 20%, 중생종이 43%, 만생종이 37%로 조사 되었으며, 엽 생장특성 중 엽폭은 19.8-75 mm의 범위를, 엽장 35.5-160 mm, 엽면적 $660-8,400\;mm^2$, 엽록소 함량(SPAD) 51.3-82.3로 비교적 변이의 폭이 넓게 조사되었다. 2. 국내 수집 보존 차나무 유전자원의 품질관련 성분검정을 수행한 결과 총질소의 함량 범위는 4.18-6.07%, 총 유리 아미노산의 함량 범위는 2.87-4.58%, 데아닌 1.64-2.66% 범위로 존재함을 확인하였다. 또한 카테킨 및 카페인 함량은 각각 11.54-15.07% 및 2.82-4.23% 범위로 존재하여 유전자원 간에 함량의 다양성이 존재함을 알 수 있다. 3. 국내 수집 재래 유전자원을 활용한 육종소재용 우수 모수 선발을 위하여 차나무 유전자원 238 계통을 대상으로 품질관련 성분 검정을 수행한 결과 총 유리 아미노산 함량이 4.2% 이상인 자원 4계통, 데아닌 함량이 2.5% 이상인 자원7계통, 카테킨 함량이 15%이상인 자원12계통, 카페인 함량이 2.75% 이하인 자원 12계통 등 총 35계통의 유망자원을 선발하였다.

The tea has traditionally been used as a foodstuff by unique flavor, however recently not only the diversity of consumer demands but also the public interest in unique favorite and functional aspects have increased. It has been also reported that the main components contained in the leaves of tea (Camellia sinensis) include total nitrogen, free amino acids, polyphenols, and fiber, of which catechin has powerful bioactive effect such as anti-cancer, anti-aging, and anti-diabetic. (-)-Epigallocatechin gallate (EGCG) which is a major phenolic constituent of green tea extract has received considerable attention for a variety of important bioactivities. This study was carried out to obtain useful information for tea breeding programs, and to investigate the concentration of quality and functional related components in Korean indigenous tea germplasms. Korean indigenous tea lines were classified into three groups of sprout time, i.e, early, medium and late sprout time, and the ratio were 20%, 43% and 37%, respectively. There was a difference in characteristics among these Korean indigenous tea lines, leaf width of those ranged from 19.8 to 75 mm, leaf length was 35.5-160.0 mm, and leaf area was $660-8,400\;mm^2$. Experimental data on chlorophyll content (SPAD value) of Korean indigenous tea genetic resources ranged from 51.3 to 82.3. The concentrations of the total nitrogen, total free amino acids, and theanine were ranged 4.18-6.07%, 2.87-4.58%, and 1.64-2.66%, respectively. Also, catechin concentration showed from 11.54 to 15.07%, and concentration of caffeine was 2.82-4.23%. These results indicated indicated that it is possible to select elite lines with high concentration of quality related components and low concentration of caffeine from Korean domestic tea germplasms.

키워드

참고문헌

  1. 송관정, 김찬식, 김영걸, 이진호. 2006. 녹차학. 제주대학교 출판부
  2. Asai, H., K. Ogawa, Y. Hara, and K. Nakamura. 1987. Effect of alumina-tea catechin complex on the blood sugar in spontaneous diabetic mice. Clin. Report 21 : 163-166
  3. Cao, J. 1995. External test and clinical observation and evaluation of the caries preventive effect of tea. The 3rd international symposium on green tea, Seoul, Korea
  4. Cho, Y. J., B. J. An, and C. Choi. 1993. Inhibition effect of against angiotensin converting enzyme of flavan-3-ols isolated Korean green tea. Korean J. Food Sci. Technol. 25 : 238-242
  5. Choi, S. I., J. H. Lee, and S. R. Lee. 1994. Effect of green tea beverage for the removal of cadmium and lead by animal experiment. Korean J. Food Sci. Technol. 26 : 745-749
  6. Fukai, K., T. Ishigami, and Y. Hara. 1991. Antibacterial activity of tea polyphenols against phytopathogenic bacterial. Agric. Biol. Chem. 55 : 1985-1897 https://doi.org/10.1271/bbb1961.55.1985
  7. Hara, Y., S. Maysuzaki, and K. Nakamura. 1989. Antitumor activity of tea catechins. Nippon Eiyo Shokuryo Gakkaishi 42 : 39-45 https://doi.org/10.4327/jsnfs.42.39
  8. Hunter, O. J., J. E. Manson, M. J. Stampger, G. A. Colditz, B. Rosner, C. H. Hennekens, F. E. Speizer, and W. C. Willett. 1992. A prospective study of caffeine, coffee, tea, and breast cancer. Am. J. Epidemiol. 136 : 1000-1001
  9. Ikeda, N., H. Horie., T. Mukai, and T. Goto. 1993. Varietal difference of chemical constituents in first and autumn flushes of tea plants. J. Tea Research 77 : 13-21
  10. Ikeda, N. and Y. G. Park. 2002 . Morphological characteristics of Korean wild tea populations. Japan J. Breeding Research 4 : 193-200 https://doi.org/10.1270/jsbbr.4.193
  11. Je, S. J., Y. H. Lee, J. S. Oh, P. S. Hwang, Y. S. Chung, and D. S. Chung. 2007. Morphological classification of tea leaves collected frem the vicinity of buddhist temple in Busan and Gyeongnam. J. Kor. Tea Soc. 13 : 115-124
  12. Kim, J. K., W. S. Cha, J. K. Park, S. Y. Oh, Y. J. Cho, S. S. Chun, and C. Choi. 1997. Inhibition effect against tyrosinase of condensed tannins from Korean green tea. Korean J. Food Sci. Technol. 29 : 173-174
  13. Kim, J. H., J. Choi, and Y. G. Park. 2007. The variation of morphological and functional property components of wild tea populations of Korea. J. Kor. Tea Soc. 13 : 123-140
  14. Lee, S. H. and Y. Yamaguchi. 1997. Comparison of chemical components among spontaneous populations of Korean tea plant and Japanese green tea cultivar "Meiryoku". J. Kor. Tea Soc. 3 : 57-65
  15. Matsuzaki, T. and Y. Hara. 1985. Antioxidant activity of tea leaf catechins. Nippon Nogeikagaku Kaishi 59 : 129-134 https://doi.org/10.1271/nogeikagaku1924.59.129
  16. Millin, D. J. 1987. Factors affecting the quality of tea. In S. M. Herschdoerfer (Ed.), Quality control in the food industry. Academic press, London. pp. 127-160
  17. Mizukami, Y., K. Kohata, Y. Yamaguchi, N. Hayashi, Y. Sawai, Y. Chuda, H. Ono, H. Yada, and M. Yoshida. 2006. Analysis of acrylamide in green tea by gas chromatographymass spectrometry. J. Agric. Food Chem. 54 : 7370-7377 https://doi.org/10.1021/jf061029a
  18. Park, Y. G. 2000. Biography of tea plant. genetic studies on Camellia sinensis var. sinensis of wild tea populations in Korea. 1st Symposium on Tea Science and Culture. pp. 41- 70
  19. Park, Y. G., J. H. Kim, N. Ikeda, and D. I. Shin. 2001. Study on the origin and the transmission of Japanese and Korean tea plant. I. The morphological and genetic variation. J. Kor. Tea Soc. 7 : 143-161
  20. Park, Y. H., E. K. Won, and D. J. Son. 2002. Effect of pH on the stability of green tea catechins. J. Food Hygiene and Safety 17(3) : 117-123
  21. Ryu, B. H. and C. O. Park. 1990. Antioxidant effect of green tea extracts on enzyme activities of hairless mice skin induced in ultraviolet B light. Korean J. Food Sci. Technol. 22 : 355-361
  22. Song, Y. S., Y. H. Moon, S. K. Han, B. C. Jeong, and J. K. Bang. 2005. Morphological characteristics of progeny population in collected wild tea. J. Kor. Tea Soc. 11 : 93-105
  23. Sung, K. C. 2006. A study on the pharmaceutical characteristics and analysis of green tea extract. J. Korean Oil Chemists's Soc. 23(2) : 115-124
  24. Takeda, Y. 2007. Review of the tea breeding of Japan in the past one hundred years and its future objects. Japan J. Tea Research 103 : 19-20
  25. Yeo, S. G., C. W. Ahn, Y. W. Lee, T. G. Lee, Y. H. Park, and S. B. Kim. 1995. Antioxidative effect of tea extracts from green tea, oolong tea, and black tea. J. Korean Food Nutr. Soc. 24 : 299-304