생명과학회지 (Journal of Life Science)

  • 제18권8호
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  • Pages.1053-1058
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  • 2008
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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알코올 투여 흰쥐의 간 조직 산화스트레스에 미치는 글루타티온 고함유 효모 Saccharomyces cerevisiae FF-8 균체의 영향

Suppressive Effect of Administrated Glutathione-Enriched Saccharomyces cerevisiae FF-8 on the Oxidative Stress in Alcoholic Fatty Liver

  • 차재영 (대선주조(주) 기술연구소) ;
  • 박상현 ((주)미애부발효과학연구소) ;
  • 허진선 (동아대학교 생명자원과학대학 생명공학과) ;
  • 조영수 (동아대학교 생명자원과학대학 생명공학과)
  • Cha, Jae-Young (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Park, Sang-Hyun (Miabu Fermentation Science Research Institute, Miabu Co. Ltd.) ;
  • Heo, Jin-Sun (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Cho, Young-Su (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
  • 발행 : 2008.08.30
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초록

글루타티온은 간 질환용 치료제 내지는 영양보충제로 시판되고 있다. 이전 실험에서 글루타티온 고함유 효모 Saccharomyces cerevisiae FF-8 균체(FF-8GY) 투여가 사염화탄소 및 알코올성 유발 간독성 개선효과를 나타내었다. 따라서, 알코올성 지방간의 산화 스트레스에 미치는 영향을 조사하기 위하여 시판되고 있는 글루타티온 고함유 효모 추출물(GYE)과 본 실험실에서 분리한 글루타티온 고함유 효모 FF-8GY을 비교실험 하였다. FF-8GY 또는 GYE 투여에 의한 간 조직 중의 산화스트레스 측정지표로서는 과산화지질(TBARS) 및 글루타티온 함량을 측정하였다. TBARS 농도는 간 조직 homogenize 분획에서 알코올 투여 대조군에 비해 FF-8GY 투여 실험군에서 5%수준에서 유의적으로 감소하였으며, GYE 투여에 의해서는 감소경향을 보였다. 간 조직 microsomal 및 mitochondria 분획에서도 같은 경향이 관찰되었다. 이때 간 조직 중의 글루타티온 농도는 정상군 보다 알코올 투여 대조군에서 5%수준에서 유의적으로 감소하였으며, 이러한 감소는 GYE 투여에 의해 더욱 감소되었으나 FF-8GY 투여에 의해서 정상군 수준까지 회복되는 것으로 나타났다. 한편 간조직의 손상정도를 나타내는 또 하나의 지표인 혈청 총단백질 및 알부민 농도는 알코올 처리 대조군에서 낮게 나타난 반면 FF-8GY 투여에 의해 정상군 수준 이상으로 증가하는 것으로 나타났다. 이상의 결과에서 글루타티온 고함유 효모 Saccharomyces cerevisiae FF-8 균체(FF-8GY)의 투여에 따른 알코올성 유발 산화스트레스의 경감 효과는 시판되고 있는 글루타티온 고함유 효모 추출물 보다 더욱 현저한 개선 효과가 있는 것으로 확인되었다.

Glutathione is a well known chemotherapeutic agent for liver disease and is a popular nutritional supplement in the United States. Previous our studies reported the suppressive effects of glutathione-enriched Saccharomyces cerevisiae FF-8 strain (FF-8GY) on carbon tetrachloride- and alcohol-induced hepatotoxicity. The primary objective of this study was to investigate the comparative effects of FF-8GY and commercially available glutathione-enriched yeast extract (GYE) against the oxidative stress in alcohol-induced fatty liver of rats. The lipid peroxidative index (thiobarbituric acid-reactive substances, TBARS) and antioxidant status (reduced glutathione level) were used to monitor those protective roles of FF-8GY or GYE treatment. When the rat was treated alcohol, the TBARS levels in the whole liver and the subfractions of microsomal and mitochondria were significantly increased but these were significantly decreased by FF-8GY treatment and tended to be lowered by GYE treatment. The concentration of hepatic glutathione is known to be closely associated with antioxidant system and this was slightly deplete in the alcohol-induced rats, but this was recovered by treating with FF-8GY. However, the glutathione concentration was more significantly decreased in the GYE supplementation in alcohol feeding rats. Alcohol treatment also negatively affected the serum total protein and albumin, but these were significantly increased near normal levels in FF-8GY coadministered rats. These results suggest that glutathione-enriched Saccharomyces cerevisiae FF-8 strain may have positively mediate the alcohol-induced oxidative stress, and this effect was more pronounced in FF-8GY compared to GYE.

키워드

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