한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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액체배양 버들송이 균사체의 칼슘흡수 및 생육특성

Calcium Absorption and Growth Characteristics of Agrocybe cylindracea Mycelia in Submerged Culture

  • 박신 (대구대학교 생명환경학부) ;
  • 공지원 (대구대학교 생명환경학부) ;
  • 이경석 (대구대학교 생명환경학부)
  • Park, Shin (Division of Life and Environmental Science, Daegu University) ;
  • Gong, Ji-Won (Division of Life and Environmental Science, Daegu University) ;
  • Lee, Kyoung-Seok (Division of Life and Environmental Science, Daegu University)
  • 발행 : 2008.08.01
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초록

액체배양 버들송이 균사체의 생육과 칼슘 흡수에 미치는 칼슘원의 영향을 조사하였다. 칼슘원의 종류에 따른 균사체의 건물량은 유의수준 5%에서 유의하지 않았으며, 칼슘원의 농도에 따른 균사체의 건물량은 농도가 500mg/L까지는 대체로 증가하는 경향이었으나 1,000mg/L 이상에서는 감소하는 경향을 보였다. 균사체의 칼슘 함량은 칼슘원의 종류와 농도에 따라 큰 차이를 보였으며, 칼슘원을 첨가하지 않고 배양한 대조구의 Ca 함량은 198.3 mg/kg이었는데 비해 칼슘을 첨가하고 배양한 경우 최소 273.7 mg/kg(1.4배)에서 최대 67,246.0 mg/kg(339.1배)까지 증가하였다. 칼슘원의 농도에 따른 균사체의 Ca 함량은 칼슘원의 농도가 증가함에 따라 균사체의 Ca 함량도 증가하였다. 배양일수에 따른 균사체의 생육은 배양 8-12일 사이 균사체의 생육속도가 가장 높았으며 16일차까지도 비교적 높은 증가율을 보였다. 배양일수에 따른 균사체의 Ca 함량은 배양기간이 짧은 어린 균사체의 경우 배양기간이 긴 성숙된 균사체에 비해 단위 건물량 당 Ca 함량이 높았다. pH에 따라서는 pH 7.0의 MCM 액체배지에서 생육이 가장 활발하였으며 Ca 함량도 가장 높았다. 결론적으로 칼슘 함량이 높은 버들송이 균사체를 생산하기 위해서 체내 흡수율이 좋고 최근 식품의 칼슘첨가제로 많이 이용되는 Ca-lactate 1,000 mg/L를 첨가한 MCM 액체배지에서 pH 조정없이 배양하여 12-16일차의 균사체를 이용하는 것이 가장 효율적이라고 판단된다.

The present study examined the effects of different calcium (Ca) sources and concentrations on the growth and Ca absorption of Agrocybe cylindracea mycelia grown in submerged cultures. The dry weights of the mycelia were not significantly different (significance level of 5%) according to the type of Ca added, and increased with increasing Ca concentration until 500 mg/L, and then decreased at concentrations of 1000 mg/L or greater. The Ca contents of groups were significantly different according to the various concentrations of the Ca source, in which the Ca content of the control group cultured without added Ca was 198.3 mg/kg, and in the treatment groups, Ca content increased to a minimum of 273.7 mg/kg (1.4 times) and a maximum of 67246.0 mg/kg (339.1 times) the Ca contents of the groups generally increased with increasing Ca concentration. According to the number of culture days, growth rates were highest during days 8 through 12, and remained relatively high until day 16. In addition, Ca contents per unit dry weight were higher in young mycelia with a shorter culture period than in mature mycelia with a longer culture period. According to pH, the most active growth and highest Ca content occurred in MCM liquid medium at pH 7.0. In conclusion, in order to produce Agrocybe cylindracea mycelia with high Ca content, it is considered most efficient to culture them in MCM liquid medium without a pH adjustment and containing 1,000 mg/L of Ca-lactate, which is commonly used as a Ca additive in food, as well as to use mycelia between 12-16 days of culturing.

키워드

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