Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inhibiting Effect of Injinoryung-san-Ga-Samchilgun on Liver Fibrosis in Rats

인진오령산가삼칠근이 흰쥐의 간섬유화 억제에 미치는 영향

  • Kim, Dong-Woo (Department of Internal Medicine, College of Oriental Medicine, Semyung University) ;
  • Kim, Young-Chul (Department of internal Medicine, College of Oriental Medicine, Kyunghee University) ;
  • Ko, Heung (Department of Internal Medicine, College of Oriental Medicine, Semyung University)
  • 김동우 (세명대학교 내과학교실) ;
  • 김영철 (경희대학교 내과학교실) ;
  • 고흥 (세명대학교 내과학교실)
  • Published : 2008.08.25
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to investigate that Injinoryung-san-Ga-Samchilgun(IJORS) has an inhibitory effect on the development of liver fibrosis in rats. The influence of IJORS on liver stellate cell viability in rat was measured by the MTT assay, and proliferation was measured by the BrdU assay. The mRNA expression of procollagen type $1{\alpha}2$, ${\alpha}-SMA$, TIMP1, and TIMP2 all of which are associated with liver fibrosis, were analyzed by RT-PCR. The inhibitory effect of IJORS on procollagen production in hepatic stellate cell was examined using by enzyme immuno assay(procollagen Type 1 C-Peptide EIA). And after IJORS was orally administered to experimental rats with thioacetamide(TAA)-induced liver fibrosis for 4 weeks, the body weight, liver function test, complete blood and the change of portal pressure were measured. IJORS prevented hepatic stellate cell viability and proliferation in a dose-dependent manner. IJORS reduced the mRNA expression of procollagen type $1{\alpha}2$, ${\alpha}-SMA$ and TIMP1 and the production of procollagen protein. IJORS inhibited the increase of AST, ALT, WBC and portal pressure in rats administered by TAA. IJORS is considered to prevent liver fibrosis by inhibiting the activation of stellate cell and production of procollagen and prevent the progress of liver fibrosis by inhibiting the inflammation of liver tissue complicated in many liver disease.

Keywords

References

  1. 이문호. 내과학(上). 서울, 학문사, p 1015, 1986
  2. Ramzi, S., Cotran Vinay, Kumar Stanley, L. Robbins. Pathologic Basis of Disease. 5th edition. Philadelphia: W.B. Saunders, pp 834-835, 1992
  3. 전국한의과대학 간계내과학교수. 간계내과학. 서울, 동양의학연구원, pp 323-355, 2001
  4. 대한가정의학회. 가정의학 임상편. 서울, 계축문화사, pp 993-999, 2002
  5. 禹弘楨. 茵蔯五苓散과 茵蔯增量한 構成方이 흰쥐 肝損傷에 미치는 影響. 대한한의학회지 113(1):234-241, 1992
  6. 안덕균 외. 原色 韓國本草圖鑑. 서울, (주)교학사, p 523, 2002
  7. Won-Hwan Park, Soo-Kyung Lee and Cheorl-Ho Kim. A Korean herbal medicine, Panax notoginseng, prevents liver fibrosis and hepatic microvascular dysfunction in rats. Life Sciences. 76(15):1675-1690, 2005 https://doi.org/10.1016/j.lfs.2004.07.030
  8. Liu Jie, Liu Yaping and Curtis D. Klaassen. The effect of Chinese hepatoprotective medicines on experimental liver injury in mice. Journal of Ethnopharmacology. 42(3):183-191, 1994 https://doi.org/10.1016/0378-8741(94)90084-1
  9. 통계청. 사망원인통계연보. 2006
  10. Iredale, J.P., Arthur, M.J. Hepatocyte-matrix interactions. Gut. 35: 729-732, 1994 https://doi.org/10.1136/gut.35.6.729
  11. Isao Sakaida, Yasuhiro Matsumura, Shinta Akiyama, Koji Hayashi, Atsushi Ishige and Kiwamu Okita. Herbal medicine Sho-saiko-to (TJ-9) prevent liver fibrosis and enzyme-altered lesions in rat liver cirrhosis induced by a choline-deficient -amino acid-defined diet. Journal of Hepatology 28(2):298-306, 1998 https://doi.org/10.1016/0168-8278(88)80017-5
  12. 김영철, 이장훈, 우홍정, 인진이 간성상세포의 섬유화 억제에 미치는 영향에 대한 연구. 대한한방내과학회지 26(4):853- 863, 2005
  13. 심재옥, 김영철, 이장훈, 우홍정. 茵蔯淸肝湯이 TGF-1 매개성간섬유화에 미치는 영향. 대한한의학회지 24(2):1-11, 2003
  14. 강중원, 최도영, 박동석, 이재동. 五苓散加減方이 3T3-L1 adipocyte의 leptin 및 leptin receptor 함량과 differentiaion에 미치는 영향. 대한한의학회지 26(2):241-251, 2005
  15. 김호현, 신홍묵, 김길훤. 茵蔯五苓散이 흰쥐의 腎毒性에 미치는 影響. 대한한의학회지 17(2):133-144, 1996
  16. Iredale, J.P., Benyon, R.C., Pickering, J., McCullen, M., Northrop, M., Pawley, S. et al. Mechanisms of spontaneous resoluyion of rat liver fibrosis. Hepatic stellate cell apoptosis and reduced hepatic expression of metalloproteinase inhibitors. J Clin Invest. 102: 538-549, 1998 https://doi.org/10.1172/JCI1018
  17. A. Castilla, J. Prieto and N. Fausto. Transforming growth factors $\beta$1 and $\alpha$ in chronic liver diseases. N Engl J Med. 324: 933-940, 1991 https://doi.org/10.1056/NEJM199104043241401
  18. Benyon, R.C., Hovell, C.J., Da Gaca, M., Jones, E.H., Iredale, J.P., Arthur, M.J. Progelatinase A is produced and activated by rat hepatic stellate cells and promotes their proliferation. Hepatology 30: 977-986, 1999 https://doi.org/10.1002/hep.510300431
  19. Muller, A., Machinik, F., Zimmermann, T., Schubert, H. Thioacetamide-induced cirrhosis-like liver lesions in rats usefulness and reliability of this animal model. Exp Pathol. 34: 229-236, 1988 https://doi.org/10.1016/S0232-1513(88)80155-5
  20. Rockey, D.C., Weisiger, R.A. Endothelium induced contractility of stellate cells from normal and cirrhotic rat liver: implication for regulation of portal pressure and resistance. Hepatology. 24: 233-240, 1996 https://doi.org/10.1002/hep.510240137
  21. Rockey, D.C., Housset, C.N., Friedman, S.L. Activation-dependent contractility of rat hepatic lipocytes in culture and in vivo. J Clin Invest. 92: 1795-1804, 1993 https://doi.org/10.1172/JCI116769
  22. Lee, J.S., Kang Decker N.K., Chatterjee, S., Yao, J., Friedman, S., Shah, V. Mechanisms of nitric oxide interplay with Rho GTPase family members in modulation of actin membrane dynamics in pericytes and fibroblast. AM J Pathol. 166: 1861-1870, 2005 https://doi.org/10.1016/S0002-9440(10)62495-9