Anti-inflammatory Effect of Evodia Officinalis $D_{ODE}$ in Mouse Macrophage and Human Vascular Endotherial Cells

마우스 대식세포 및 사람 혈관 내피세포에서 오수유(Evodia officinalis $D_{ODE}$) 메탄올 추출물의 항염증 효과

  • Yun, Hyun-Jeung (Cardiovascular Medical Research Center and Department of Prescriptionology, Department Prescriptionology Collage of Oriental Medicine, Dongguk University) ;
  • Heo, Sook-Kyoung (Cardiovascular Medical Research Center and Department of Prescriptionology, Department Prescriptionology Collage of Oriental Medicine, Dongguk University) ;
  • Lee, Young-Tae (Department Prescriptionology Collage of Oriental Medicine, Dongguk University) ;
  • Park, Won-Hwan (Cardiovascular Medical Research Center and Department of Prescriptionology) ;
  • Park, Sun-Dong (Cardiovascular Medical Research Center and Department of Prescriptionology, Department Prescriptionology Collage of Oriental Medicine, Dongguk University)
  • 윤현정 (심혈관계질환 천연물연구개발센터, 동국대학교 한의과대학 방제학교실) ;
  • 허숙경 (심혈관계질환 천연물연구개발센터, 동국대학교 한의과대학 방제학교실) ;
  • 이영태 (동국대학교 한의과대학 방제학교실) ;
  • 박원환 (심혈관계질환 천연물연구개발센터) ;
  • 박선동 (심혈관계질환 천연물연구개발센터, 동국대학교 한의과대학 방제학교실)
  • Published : 2008.03.30

Abstract

Objectives : Evodia officinalis DODE (EO), an herbal plant, has been widely used in traditional Korean medicine for the treatment of vascular diseases such as hypertension. The crude extract of EO contains phenolic compounds that are effective in protecting liver microsomes, hepatocytes, and erythrocytes against oxidative damage. But EO has been little found to have an anti-inflammatory activity. We investigated anti-inflammatory activity of EO in RAW 264.7 cells and human umbilical vein endothelial cells (HUVECs). Methods : Cytotoxic activity of EO on RAW 264.7 cells was investigated by using 5-(3-caroboxymeth-oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. The nitric oxide (NO) production was measured by Griess reagent system. And proinflammatory cytokines were measured by ELISA kit. The levels of intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression were measured by flow cytometer. Results : EO decreased LPS-induced NO production in RAW 264.7 cells. The inhibitory activity of EO on LPS-induced NO release is probably associated with suppressing TNF-${\alpha}$, IL-6 and MCP-1 formation. These results indicate that EO has potential as an anti-inflammatory agent. Moreover, EO decreased TNF-${\alpha}$-induced IL-8, IL-6 production, and ICAM-1 and VCAM-1 expression in HUVECs. Conclusions : EO inhibits TNF-${\alpha}$-induced inflammation via decreasing cytokines production and adhesion molecules expression. These results indicate that EO has potential as an anti-inflammation and anti-artherosclerosis agent.

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