생명과학회지 (Journal of Life Science)

  • 제18권9호
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  • Pages.1290-1298
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  • 2008
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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송이(Tricholoma matsutake)의 자실체에서 추출한 조다당류가 생쥐의 Sarcoma 180에 미치는 항암 및 면역증강 작용

Antitumor and Immuno-potentiating Activity against Mouse Sarcoma 180 by Crude Polysaccharides Extracted from Fruiting Body of Tricholoma matsutake

  • 허현 (동국대학교 생명과학과) ;
  • 최연일 (인천대학교 생물학과) ;
  • 이태수 (인천대학교 생물학과)
  • Hur, Hyun (Department of Life Science, Dongguk University) ;
  • Choi, Yon-Il (Department of Biology, University of Incheon) ;
  • Lee, Tae-Soo (Department of Biology, University of Incheon)
  • 발행 : 2008.09.30
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초록

송이는 담자균문, 주름버섯목, 송이과에 속하며 맛이 좋은 식용 버섯으로 예로부터 사람의 위궤양이나 위암의 치료효과는 물론 생쥐의 Sarcoma 180과 Ehrlich Sarcoma에도 효과가 있다고 알려져 있다. 본 연구에서는 송이의 자실체로부터 중성염용액, 열수 및 메탄올을 이용하여 조다당류를 추출하고 ICR mice에 주사하여 항암 및 면역증강 효과를 조사하였다. NIH3T3, Sarcoma 180, HepG2, HT-29 등의 암세포에 대한 독성을 조사한 결과 각각의 암세포는 2 mg/ml의 조다당류 농도에서는 세포독성을 나타내지 않았다. Sarcoma 180이 접종된 ICR mouse에 자실체에서 추출한 각각의 조다당류를 투여한 실험군은 대조군에 비해 평균수명이 각각 23.4$\sim$37.2% 연장되었다. 메탄올과 열수로 추출한 조다당류를 0.2$\sim$0.5 mg/ml의 농도로 투여한 실험군 생쥐의 B 임파구 alkaline phosphatase 활성은 대조군에 비해 각각 2.17$\sim$11.9배의 증가하였다. 중성염추출 조다당류를 50 mg/kg body weight의 농도로 투여한 생쥐의 총 복강 세포 수와 백혈구의 수는 대조군에 비하여 각각 6배와 1.5배 증가하였다. 따라서 송이의 자실체에서 추출한 조다당류는 생쥐의 Sarcoma 180에 대해 항암작용 및 면역증강 작용을 나타내는 것으로 확인되었다.

Tricholoma matsutake, one of edible and medicinal mushroom belonging to Tricholomaceae of Agaricales, has been known to contain some curing effect on gastric cancer and ulcer, and inhibitory effect on sarcoma 180 and Ehrlich sarcoma. Neutral salt soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180, HepG2, HT29 and NIH3T3 at the concentration of 2.0 mg/ml. Intraperitoneal injection with crude polysaccharides showed life prolongation effect of 23.4$\sim$37.2% in mice previously inoculated with Sarcoma 180. Fr. MeOH and Fr. HW exhibited the immuno-potentiating activity of B lymphocyte by increasing the alkaline phosphatase activity by 2.2$\sim$11.9 folds compared with control at the concentration of 0.2$\sim$0.5 mg/ml. In case of Fr. NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 6.0 and 1.5 folds at the concentration of 50 mg/kg, respectively. Therefore, it is concluded that crude polysaccharides extracted from fruiting body of Tricholoma matsutake showed antitumor and immuno-potentiating activity against Sarcoma 180 of mouse.

키워드

참고문헌

  1. Allison, D. C. and P. Ridolpho. 1980. Use of a trypan blue assay to measure the deoxyribonucleic acid content and radioactive labeling of viable celIs. J. Histochem. Cytochem. 28, 700-703. https://doi.org/10.1177/28.7.6156203
  2. Arthur, C. and M. D. Guyton. 1986. Textbook of medical physiology. 7th Eds. W. B. Saunder Company. pp. 51-59.
  3. Cho, S. M., J. H. Lee, S. B. Han, H. M. Kim, S. H. Yoo and J. D. Yoo. 1995a. Immuno- stimulating Polysaccharides from the Fruiting Bodies of Fomitella fraxinea (1)Characterization of polysaccharides extracted with neutral sodium chloride solution. The Korean Journal of Mycology 23, 332-339.
  4. Cho, S. M., J. H. Lee, S. B. Han, H. M. Kim, S. H. Yoo and I. D. Yoo. 1995b. Immuno- stimulating Polysaccharides from the Fruiting Bodies of Fomitella fraxinea (2)- Isolation and characterization of hot- water extracted polysaccharides . The Korean Journal of Mycology 23, 340-347.
  5. Choi, Y. I., K. W. Lee, H. Hur, W. Y. Lee and T. S. Lee. 2008. Immuno-potentiating and Antitumor Effects against Mouse Sarcoma 180 by Crude Polysaccharides Extracted from Fruiting Body of Russula rosacea. The Korean Journal of Mycology 36, 84-92. https://doi.org/10.4489/KJM.2008.36.1.084
  6. Denizot, F. and R. Lang. 1986. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89, 271-277. https://doi.org/10.1016/0022-1759(86)90368-6
  7. Fukuda, K., T. Uematsu, A. Hamada and S. Akiya. 1975. The polysaccharide from Lampteromyces Japonicus. Chem. pharm. Bull. 23, 1955-1961. https://doi.org/10.1248/cpb.23.1955
  8. Ji, J. H. and M. N. Kim. 2000. Antimutagenic and cytotoxicity effects of Phellinue linteus extracts. J. Kor. Soc. Food Sci. Nutr. 29, 322-330.
  9. Jin, M. L. 1996. A Study on the Activation of Immune Cell and Transcription Factor of Pine Nut Mushroom. Collected Papers of Seoul National University Graduate School. pp. 1-121.
  10. Kim, S. B., K. W. Lee, H. Y. Kim, M. J. Shim, H. S. Rho, H. S. Lee, M. W. Lee, W. Y. Lee and T. S. Lee. 2006. Articles: Inhibitive effect of mouse sarcoma 180 by crude polysaccharide extracted from fruiting Body of Aramillaria mellea. The Korean Journal of Mycology 34, 98-104. https://doi.org/10.4489/KJM.2006.34.2.098
  11. Kim S. B., K. W. Lee, W. Y. Lee and T. S. Lee. 2007. Articles: Studies on immuno-modulatory and antitumor effects of crude polysaccharides extracted from fruiting Body of Oudemansiella radicata, The Korean Journal of Mycology 35, 109-114. https://doi.org/10.4489/KJM.2007.35.2.109
  12. Lee, K. W., H. Y. Kim, W. H. Lee and T. S. Lee, 2007. Antitumor and immune-modulatory effect against mouse sarcoma 180 of crude polysaccharides extracted from fruiting Body of Armillaria tabescens. The Korean Journal of Mycology 35, 101-108. https://doi.org/10.4489/KJM.2007.35.2.101
  13. Lee, K. W., H. Y. Kim, H. Hur, M. W. Lee, M. J. Shim, W. Y. Lee and T. S. Lee. 2008. Antitumor and immuno-modulatory effect of crude polysaccharides from fruiting body of Tremella aurantialba against mouse sarcoma 180. The Korean Journal of Mycology 36, 66-74. https://doi.org/10.4489/KJM.2008.36.1.066
  14. Lee, K. Y., M. J. Han, S. Y. Park and D. H. Kim. 2000. In vitro and in vivo antitumor activity of the fruit body of Phellinus linteus. Kor. J. Food Sci. Technol. 32, 477-480.
  15. Mossman, B. T. 1983. In vitro approaches for determining mechanisms of toxicity and carcinogenicity by asbestos in the gastrointestinal and respiratory tracts. Environ. Health Perspect 53, 155-161. https://doi.org/10.2307/3429628
  16. Oh, Y. H., W. Y. Lee, M. W. Lee, M. J. Shim and T. S. Lee. 2004. Immuno-modulatory and antitumor effect of crude polysaccharides extracted from sclerotium of Grifola umbellata. The Korean Journal of Mycology 32, 23-30. https://doi.org/10.4489/KJM.2004.32.1.023
  17. Oh, Y. H., S. B. Kim, K. W. Lee, H. Y. Kim, M. J. Shim, H. S. Rho, H. S. Lee, M. W. Lee, W. Y. Lee and T. S. Lee. 2006. The immuno-modulatory and antitumor effects of crude polysaccharides extracted from Tremella fuciformis. The Korean Journal of Mycology 34, 105-111. https://doi.org/10.4489/KJM.2006.34.2.105
  18. Park, W. H. and H. D. Lee. 2002. Mushrooms in Korea. Kyo-Hak Publishing Co., Ltd., Seoul.
  19. Shim, S. M., K. H. Im, W. Y. Lee, J. W. Kim, M. J. Shim, M. W. Lee and T. S. Lee. 2003a. Studies on immune-modulatory and antitumor effects of crude polysaccharides extracted from Paecilomyces sincluirii. The Korean Journal of Mycology 31, 155-160. https://doi.org/10.4489/KJM.2003.31.3.155
  20. Shim, S. M., K. W. Im, J. W. Kim, W. Y. Lee, H. W. Kim, M. W. Lee and T. S. Lee. 2003b. The immuno-modulatory and antitumor effects of crude polysaccharides extracted from Daedaleopsis tricolor. The Korean Journal of Mycology 31, 161-167. https://doi.org/10.4489/KJM.2003.31.3.161
  21. Suzuki, M., T. Arika, T. Ameriya and M. Fujiwara. 1982. Cooperative role of T lymphocytes and macropages in antitumor activity of mice pretreated with schizophyllan. Jpn. J. Exp. Med. 50, 59-65.
  22. Ying, J. Z., X. L. Mao, Q. M. Ma, Y. C. Zong and H. A. Wen. 1987. Icons of medicinal fungi from China. Science Press, Beijing, China.

피인용 문헌

  1. Inhibitory Effects of Cultured Tricholoma matsutake Mycelia on Melanin Biosynthesis vol.43, pp.2, 2011, https://doi.org/10.9721/KJFST.2011.43.2.240
  2. Immuno-stimulating and Antitumor Effects on Mouse Sarcoma 180 by Crude Polysaccharides Extracted from Fruiting Body of Hericium erinaceus vol.20, pp.4, 2010, https://doi.org/10.5352/JLS.2010.20.4.623
  3. Determination of Antibacterial Activity from Tricholoma matsutake Extract and Its Application to Low Salted Jeot-gal vol.30, pp.5, 2015, https://doi.org/10.7841/ksbbj.2015.30.5.253